- Volume 67, Issue 6, 2017
Volume 67, Issue 6, 2017
- New Taxa
-
- Bacteroidetes
-
-
Emticicia aquatilis sp. nov., isolated from a freshwater sample
A Gram-stain-negative, facultatively anaerobic, non-motile, rod-shaped and yellow-pigmented bacterium, designated strain THG-DN6.14T, was isolated from a freshwater sample near Donghaksa temple in Daejeon, South Korea. On the basis of the results of 16S rRNA gene sequence comparisons, THG-DN6.14T was found to be most closely related to Emticicia sediminis JBR12T (99.1 % sequence similarity), Emticicia oligotrophica DSM 17448T (97.6 %), Emticicia aquatica HMF2925T (96.5 %), and Emticicia ginsengisoli Gsoil 085T (94.4 %). The DNA–DNA relatedness between THG-DN6.14T and its phylogenetically closest neighbours was below 65.0 %. The DNA G+C content was 43.3 mol%. The major polar lipids were found to be phosphatidylethanolamine, an unidentified glycolipid and an unidentified aminoglycolipid. The major fatty acids were identified as C16 : 0, iso-C15 : 0, iso-C17 : 0 3-OH, and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c). The respiratory quinone was menaquinone MK-7. These data supported the affiliation of THG-DN6.14T to the genus Emticicia . THG-DN6.14Tcould be distinguished from related species of the genus Emticicia by physiological and biochemical tests. Therefore, the novel isolate represents a novel species, for which the name Emticicia aquatilis sp. nov. is proposed, with THG-DN6.14T (=KACC 18540T=CGMCC 1.15958T) as the type strain.
-
-
-
Flaviaesturariibacter luteus sp. nov., isolated from an agricultural floodplain soil, and emended description of the genus Flaviaesturariibacter
More LessA novel slow-growing bacterial strain designated as AW305T was isolated from an agricultural floodplain soil located in Mashare, Kavango region, Namibia. Cells stained Gram-negative, were non-motile, non-spore-forming, coccoid to rod-shaped and did not form a capsule. Colonies were yellow-pigmented, but flexirubin-type pigments were not detected. AW305T had an aerobic chemo-organoheterotrophic metabolism, using a narrow spectrum of carbon sources for growth, with preference for complex protein substrates, organic acids and amino acids. AW305T was able to grow at 15–40 °C, pH 5.3–8.3 and in the presence of up to 0.25 % (w/v) NaCl. 16S rRNA gene sequence comparison showed that AW305T belonged to the genus Flaviaesturariibacter (family Chitinophagaceae ). Its closest relatives were Flaviaesturariibacter amylovorans GCR0105T (97.0 %), Flavisolibacter ginsengiterrae Gsoil 492T (93.6 %) and Flavisolibacter ginsengisoli Gsoil 643T (93.2 %). DNA–DNA hybridization experiments corroborated that AW305T represents an independent genomospecies. The genomic DNA G+C content was 57.6 mol%. Major fatty acids were iso-C15 : 1 G, iso-C15 : 0, C16 : 1ω5c, iso-C17 : 0 3-OH and summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH). The predominant respiratory quinone was MK-7, albeit minor amounts of MK-6 were also detected. The polar lipids comprised major amounts of phosphatidylethanolamine and minor amounts of two unidentified lipids, an unidentified phospholipid, an unidentified glycolipid and an unidentified aminoglycophospholipid. On the basis of the polyphasic characterization, strain AW305T represents a novel species of the genus Flaviaesturariibacter for which the name Flaviaesturariibacter luteus sp. nov. is proposed, with the type strain AW305T (=DSM 100282T=LMG 29416T).
-
-
-
Salibacter halophilus gen. nov., sp. nov., isolated from a saltern
More LessA Gram-stain-negative and facultatively anaerobic bacterium, JZ3C34T, was isolated from a saltern in Feicheng, China (36° 8′ 24.45″ E 116° 49′ 22.46″ N). Cells of strain JZ3C34T were 0.3–0.4 µm wide and 1.5–2.0 µm long, catalase-positive and oxidase-negative. Colonies on modified marine agar 2216 were orange, circular, convex, translucent and approximately 1 mm in diameter after incubation for 96 h at 33 °C. Growth occurred at 20–50 °C (optimally at 33 °C), at pH 6.5–8.5 (optimally at 7.0–8.0) and in the presence of 2–18 % (w/v) NaCl (optimally in 6 % NaCl). Phylogenetic analysis of the 16S rRNA gene indicated that strain JZ3C34T was a member of the family Cryomorphaceae within the order Flavobacteriales and the most closely related species was Owenweeksia hongkongensis DSM 17368T (89.2 % 16S rRNA gene sequence similarity). The major respiratory quinone of strain JZ3C34T was menaquinone MK-7, and the dominant fatty acids were iso-C15 : 0 and iso-C15 : 1 G. The major polar lipids were two unidentified lipids and phosphatidylethanolamine, and the genomic DNA G+C content was 39.6 mol%. Polyphasic taxonomy clearly places the new strain as a novel species within a new genus of the family Cryomorphaceae , for which the name Salibacter halophilus gen. nov., sp. nov. The type strain of Salibacter halophilus is JZ3C34T (=KCTC 52047T=MCCC 1K02288T).
-
-
-
Aquimarina rubra sp. nov., isolated from sediment of a sea cucumber culture pond
More LessA Gram-stain-negative, non-motile, rod-shaped, red-pigmented, facultatively anaerobic bacterium, designated SS2-9T, was isolated from sediment collected from a sea cucumber culture pond located in Rongcheng, Shandong province, China. Cells of strain SS2-9T were approximately 0.3–0.5 µm in width and 1.5–6.0 µm in length. The strain was able to grow at 10–37 °C, at pH 6.5–8.5 and in the presence of 0.5–6.0 % (w/v) NaCl. It grew optimally at 28 °C and in the presence of 2.0 % (w/v) NaCl. The DNA G+C content was 34.5 mol% and the sole respiratory quinone was menaquinone 6 (MK-6). The predominant cellular fatty acids were C15 : 0, iso-C15 : 1 G, iso-C15 : 0 and iso-C17 : 0 3-OH. The major polar lipids were phosphatidylethanolamine, an unidentified phospholipid, two unidentified aminolipids and four unidentified lipids. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain SS2-9T was phylogenetically related to members of the genus Aquimarina and was closely related to Aquimarina amphilecti 92VT (97.29 % similarity). On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain SS2-9T was considered to represent a novel species of the genus Aquimarina , for which the name Aquimarina rubra sp. nov. is proposed. The type strain is SS2-9T (=KCTC 52274T=MCCC 1H00142T).
-
-
-
Reclassification of Flexibacter tractuosus NBRC 15981T as Marivirga harenae sp. nov. in the family Flammeovirgaceae
More LessFlexibacter tractuosa [Lewin, 1969] was reclassified as Marivirga tractuosa . Flexibacter tractuosus NBRC 15981T was reclassified herein by using a polyphasic taxonomic approach. Cells of the strain were strictly aerobic, Gram-stain-negative, slender rods, which were motile by gliding. The major respiratory quinone was menaquinone-7 and the predominant (>5 %) cellular fatty acids were iso-C15 : 0, iso-G-C15 : 1, C16 : 1ω7c and iso-C17 : 0 3-OH. The polar lipid pattern indicated the presence of a phosphatidylethanolamine, several unidentified aminolipids, glycolipids and five unidentified polar lipids. The G+C content of the genomic DNA was 35.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain NBRC 15981T clustered with members of the genus Marivirga in the family Flammeovirgaceae of the phylum Bacteroidetes . Levels of DNA–DNA relatedness were less than 16 % between strain NBRC 15981T and the two closely related species, Marivirga sericea NBRC 15983T and Marivirga tractuosa NBRC 15989T. Strain NBRC 15981T could be differentiated from these type strains in the genus Marivirga based on the polar lipid pattern and the activity of α-chymotrypsin, as well as by α-glucosidase and β-glucosidase activity. On the basis of these results, NBRC 15981T is proposed as representing a novel species of the genus Marivirga , named Marivirga harenae sp. nov. The type strain is JK11T (=NBRC 15981T=NCIMB 1429T).
-
-
-
Sphingobacterium alkalisoli sp. nov., isolated from a saline-alkaline soil
More LessA Gram-staining-negative, non-motile, non-spore-forming bacterium designated Y3L14T was isolated from the saline-alkaline soil of a farmland, Inner Mongolia, northern China. Strain Y3L14T could grow at 10–40 °C (optimally at 30 °C), pH 6.0–10.0 (optimally at pH 8.0), and in the presence of 0–6.0 % (w/v) NaCl (optimally with 0–2.0 %). Phylogenetic analysis based on the 16S rRNA gene and DNA gyrase subunit B (gyrB) gene sequences revealed that strain Y3L14T clustered with strains belonging to the genus Sphingobacterium , sharing the highest 16S rRNA gene sequence similarity with Sphingobacterium lactis WCC 4512T (94.99 %). Its major cellular fatty acids contained iso-C15 : 0, C16 : 0, iso-C17 : 0 3-OH and summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1ω7c). Menaquinone-7 (MK-7) was the only isoprenoid quinone. Strain Y3L14T contained phosphatidylethanolamine, sphingophospholipid, two unknown phospholipids and three unknown lipids as the major polar lipids. The genomic DNA G+C content of strain Y3L14T was 36.0 mol%. Based on the phenotypic, phylogenetic and genotypic characteristics, strain Y3L14T represents a novel species within the genus Sphingobacterium , for which Sphingobacterium alkalisoli sp. nov. is proposed; the type strain is Y3L14T (=CGMCC 1.15782T=KCTC 52379T).
-
-
-
Red-pink pigmented Hymenobacter coccineus sp. nov., Hymenobacter lapidarius sp. nov. and Hymenobacter glacialis sp. nov., isolated from rocks in Antarctica
Four rod–shaped and Gram-stain-negative bacterial strains, CCM 8647, CCM 8649T, CCM 8643T and CCM 8648T, were isolated from rock samples collected on James Ross Island, Antarctica. Extensive biotyping, fatty acid profiling, chemotaxonomy, 16S rRNA gene sequencing and whole-genome sequencing was applied to isolates to clarify their taxonomic position. Phylogenetic analysis based on 16S rRNA gene sequencing indicated that all four isolates belonged to the genus Hymenobacter . Strains CCM 8649T and CCM 8647 were most closely related to Hymenobacter arizonensis OR362-8T (94.4 % 16S rRNA gene sequence similarity), strain CCM 8643T to Hymenobacter terrae DG7AT (96.3 %) and strain CCM 8648T to Hymenobacter glaciei VUG-A130T (96.3 %). The predominant fatty acids of CCM 8649T and CCM 8647 were summed feature 3 (C16 : 1ω7c/C16 : 1ω6c), C16 : 1ω5c and iso-C15 : 0, whereas those of CCM 8643T and CCM 8648T were summed feature 3 (C16 : 1ω7c/C16 : 1ω6c) and C16 : 1ω5c. The quinone systems contained exclusively menaquinone MK-7. The major polyamine was sym-homospermidine. All four strains contained the major polar lipid phosphatidylethanolamine. The G+C content of genomic DNA ranged from 60–63 mol%. Whole-genome sequencing data supported the finding that isolates represented distinct species of the genus Hymenobacter . On the basis of the results obtained, three novel species are proposed for which the names Hymenobacter coccineus sp. nov., Hymenobacter lapidarius sp. nov. and Hymenobacter glacialis sp. nov. are suggested, with the type strains CCM 8649T (=LMG 29441T=P5239T), CCM 8643T (=LMG 29435T=P3150T) and CCM 8648T (=LMG 29440T=P5086T), respectively.
-
-
-
Polaribacter litorisediminis sp. nov., isolated from a tidal flat
More LessA Gram-stain-negative, aerobic, non-motile and ovoid or rod-shaped bacterial strain, designated OITF-11T, was isolated from a tidal flat in Oido, an island of South Korea, and subjected to a polyphasic taxonomic study. Strain OITF-11T grew optimally at 25 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. The phylogenetic trees based on 16S rRNA gene sequences showed that strain OITF-11T belonged to the genus Polaribacter . Strain OITF-11T exhibited 16S rRNA gene sequence similarity values of 97.4–98.1 % to Polaribacter haliotis RA4-7T, Polaribacter atrinae KACC 17473T, Polaribacter dokdonensis DSW-5T and Polaribacter marinaquae KCTC 42664T, and of 94.1–96.9 % to the type strains of the other Polaribacter species. Strain OITF-11T contained menaquinone MK-6 as the predominant menaquinone and iso-C15 : 0, iso-C15 : 1 G, iso-C15 : 0 3-OH, iso-C17 : 0 3-OH and C15 : 1ω6c as the major fatty acids. The major polar lipids detected in strain OITF-11T were phosphatidylethanolamine, phosphatidylmonomethylethanolamine and one unidentified lipid. The DNA G+C content of strain OITF-11T was 32.2 mol% and its DNA–DNA relatedness values with the type strains of P. haliotis , P. atrinae , P. dokdonensis and P. marinaquae were 14–33 %. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain OITF-11T is separated from recognized species of the genus Polaribacter . On the basis of the data presented, strain OITF-11T is considered to represent a novel species of the genus Polaribacter , for which the name Polaribacter litorisediminis sp. nov. is proposed. The type strain is OITF-11T (=KCTC 52500T=NBRC 112457T).
-
- Firmicutes and Related Organisms
-
-
Marinisporobacter balticus gen. nov., sp. nov., Desulfosporosinus nitroreducens sp. nov. and Desulfosporosinus fructosivorans sp. nov., new spore-forming bacteria isolated from subsurface sediments of the Baltic Sea
More LessFour novel Gram-stain-positive, endospore-forming bacteria of the order Clostridiales were isolated from subsurface sediments sampled during International Ocean Discovery Program Expedition 347 to the Baltic Sea. One strain (59.4MT) grew as an obligate heterotroph by aerobic respiration and anaerobically by fermentation. Optimum growth was observed with 0.5 % NaCl at 25 °C and pH 7.0–7.3. Analysis of 16S rRNA gene sequences of 59.4MT revealed Alkaliphilus transvaalensis (92.3 % identity), Candidatus Geosporobacter ferrireducens (92.2 %), Geosporobacter subterraneus (91.9 %) and Alkaliphilus peptidifermentans (91.7 %) to be the closest relatives. On the basis of the results of phenotypic and genotypic analyses, we propose that strain 59.4MT represents a novel species within a novel genus, Marinisporobacter balticus gen. nov., sp. nov., with the type strain 59.4MT (=DSM 102940T=JCM 31103T). Three other strains, 59.4F, 59.4BT and 63.6FT, were affiliated with the genus Desulfosporosinus and grew as strictly anaerobic sulfate reducers. These strains additionally used thiosulfate, elemental sulfur, sulfite and DMSO as electron acceptors and hydrogen as an electron donor. Strains 59.4F and 59.4BT had identical 16S rRNA gene sequences, which were most similar to those of Desulfosporosinus lacus (97.8 %), Desulfosporosinus hippei (97.3 %) and Desulfosporosinus orientis (97.3 %). Strain 63.6FT was closely related to D. lacus (97.7 %), Desulfosporosinus meridiei (96.6 %) and D. hippei (96.5 %). The similarity of 16S rRNA gene sequences of strains 59.4BT and 63.6FT was 96.6 %. We propose the new names Desulfosporosinus nitroreducens sp. nov., incorporating strain 59.4F (=DSM 101562=JCM 31104) and the type strain 59.4BT (=DSM 101608T=JCM 31105T), and Desulfosporosinus fructosivorans sp. nov., with the type strain 63.6FT (=DSM 101609T=JCM 31106T).
-
-
-
Paenibacillus apis sp. nov. and Paenibacillus intestini sp. nov., isolated from the intestine of the honey bee Apis mellifera
More LessThree strains, CBM1T, CBH23 and LAH16T, belonging to the genus Paenibacillus were isolated from the midgut and hindgut of Apis mellifera. Based on 16S rRNA gene sequence analyses and phenotypic characteristics, the three strains represent two novel species. Strains CBM1T and CBH23 formed a group with Paenibacillus puldeungensis CAU 9324T, and strain LAH16T belonged to the Paenibacillus amylolyticus NRRL NRS-290T subgroup of the genus Paenibacillus . The DNA G+C contents of strains CBM1T, CBH23 and LAH16T were 47.7, 48.1 and 46.1 mol%, respectively. The three strains possessed diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine as polar lipids. The predominant quinone in the three strains was MK-7, but strains CBM1T and CBH23 contained an additional major quinone, MK-8(H2). While strain LAH16T and most species of the genus Paenibacillus possessed anteiso-C15 : 0, iso-C16 : 0 and C16 : 0 as major cellular fatty acids, strains CBM1T and CBH23 possessed C16 : 0, anteiso-C15 : 0, C18 : 1ω7c and C19 : 0cyclo ω8c. Based on phenotypic, chemotaxonomic and phylogenetic data, strains CBM1T and CBH23 and the strain LAH16T represent novel species in the genus Paenibacillus , for which the names Paenibacillus apis sp. nov. and Paenibacillus intestini sp. nov. are proposed, with CBM1T (=KCTC 33844T=JCM 31620T) and LAH16T (=KCTC 33832T=JCM 31621T) as the type strains, respectively.
-
-
-
Enterococcus crotali sp. nov., isolated from faecal material of a timber rattlesnake
A facultatively anaerobic, Gram-stain-positive bacterium, designated ETRF1T, was found in faecal material of a timber rattlesnake (Crotalus horridus). Based on a comparative 16S rRNA gene sequence analysis, the isolate was assigned to the genus Enterococcus . The 16S rRNA gene sequence of strain ETRF1T showed >97 % similarity to that of the type strains of Enterococcus rotai , E. caccae , E. silesiacus , E haemoperoxidus , E. ureasiticus , E. moraviensis , E. plantarum , E. quebecensis , E. ureilyticus , E. termitis , E. rivorum and E. faecalis . The organism could be distinguished from these 12 phylogenetically related enterococci using conventional biochemical testing, the Rapid ID32 Strep system, comparative pheS and rpoA gene sequence analysis, and comparative whole genome sequence analysis. The estimated in silico DNA–DNA hybridization values were <70 %, and average nucleotide identity values were <96 %, when compared to these 12 species, further validating that ETRF1T represents a unique species within the genus Enterococcus . On the basis of these analyses, strain ETRF1T (=CCUG 65857T=LMG 28312T) is proposed as the type strain of a novel species, Enterococcus crotali sp. nov.
-
-
-
Alkaliphilus namsaraevii sp. nov., an alkaliphilic iron- and sulfur-reducing bacterium isolated from a steppe soda lake
A novel alkaliphilic spore-forming bacterium was isolated from the benthic sediments of the highly mineralized steppe Lake Khilganta (Transbaikal Region, Russia). Cells of the strain, designated Х-07-2T, were straight to slightly curved rods, Gram-stain-positive and motile. Strain Х-07-2T grew in the pH range from 7.0 to 10.7 (optimum pH 9.6–10.3). Growth was observed at 25–47 °C (optimum 30 °C) and at an NaCl concentration from 5 to 150 g l−1 with an optimum at 40 g l−1. Strain Х-07-2T was a chemo-organoheterotroph able to reduce amorphous ferric hydroxide, Fe(III) citrate and elemental sulfur in the presence of yeast extract as the electron donor. It used tryptone, peptone and trypticase with Fe(III) citrate as the electron acceptor. The predominant fatty acids in cell walls were C16:1ω8, iso-C15:0, C14 : 0 3-OH and C16 : 0. The DNA G+C content was 32.6 mol%. 16S rRNA gene sequence analysis revealed that strain Х-07-2T was related most closely to members of the genus Alkaliphilus within the family Clostridiaceae . The closest relative was Alkaliphilus peptidifermentans Z-7036T (96.4 % similarity). On the basis of the genotypic, chemotaxonomic and phenotypic data, strain Х-07-2T represents a novel species in the genus Alkaliphilus , for which the name Alkaliphilus namsaraevii sp. nov. is proposed. The type strain is Х-07-2T (=VKM В-2746Т=DSM 26418Т).
-
- Other Bacteria
-
-
Desulfurobacterium indicum sp. nov., a thermophilic sulfur-reducing bacterium from the Indian Ocean
A novel sulfur-reducing bacterium, strain K6013T, was isolated from a sulfide sample collected at a depth of 2771 m from a high-temperature hydrothermal vent in the Indian Ocean. Cells were Gram-stain-negative, anaerobic, motile rods (0.9–2.2×0.4–0.6 µm). The strain grew at NaCl concentrations ranging from 1 to 4.5 % (w/v) (optimum 2.5 %), at pH 5 to 8 (optimum pH 6), and at temperatures between 40 and 75 °C (optimum 65 °C). K6013T was an obligate chemolithoautotroph, using thiosulfate, sulfur and nitrate as terminal electron acceptors in the presence of H2 but not sulfate, sulfite nor nitrite. The major cellular fatty acids were C16 : 0 (17.4 %), C18 : 1ω7c/C18 : 1ω6c (ummed feature 8, 37.91 %), C18 : 0 (18.29 %) and C14 : 0 3-OH/iso-C16: 1I (summed feature 2, 8.56 %). The DNA G+C content was 38.2 mol%. The results of phylogenetic 16S rRNA gene sequence analyses indicated that K6013T represents a member of the genus Desulfurobacterium within the class Aquificae , with highest sequence similarity of 96.93 % to Desulfurobacterium atlanticum SL22T. On the basis of genotypic and phenotypic data, K6013T is considered to represent a novel species of the genus Desulfurobacterium , for which the name Desulfurobacterium indicum sp. nov. is proposed, with the type strain K6013T (=DSM 101677T=MCCC 1A01868T).
-
- Proteobacteria
-
-
Salinovum rubellum gen. nov., sp. nov., isolated from sediment of a saltern
More LessAn aerobic, Gram-reaction-negative, oxidase-positive and catalase-positive strain, YCWB25T, was isolated from saltern farm sediment in Jimo-Daqiao of Qingdao, east coast of China. This strain had an absolute requirement for NaCl and was able to grow in the presence of 1–10 % (w/v) NaCl (optimal growth at 3–5 % NaCl). The strain grew at pH 5.0–9.0 (optimal growth at pH 8.0) and 4–42 °C (optimal growth at 30 °C). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YCWB25T belonged to the Roseobacter clade of the family Rhodobacteraceae , and the closest species were members of the genus Pseudooceanicola (similarity lower than 95.58 %). However, the novel strain formed a robust, distinct clade different from other members of the Roseobacter clade. The DNA G+C content of strain YCWB25T was 65.6 mol%. The predominant respiratory quinone was Q-10. The dominant cellular fatty acids were C18 : 1ω7c, C16 : 0 and C19 : 0 cyclo ω8c. The phenotypic, chemotaxonomic and phylogenetic data indicated that strain YCWB25T represents a novel species of a new genus, for which the name Salinovum rubellum gen. nov., sp. nov. is proposed. The type strain of Salinovum rubellum is YCWB25T (=MCCC 1K00272T=LMG 25394T).
-
-
-
Nitratireductor aestuarii sp. nov., a marine alphaproteobacterium isolated from an estuary
More LessTwo bacterial strains, 2-2-12-1T and 2-2-12-2, were isolated from the estuary of the Jiulong River, south-east China. Cells were Gram-stain-negative, non-motile, short rods without flagella. Growth occurred at 25–45 °C, at pH 5.0–9.0 and with 0.5–2 % (w/v) NaCl. The bacteria were unable to reduce nitrate. The predominant fatty acids were C19 : 0 cyclo ω9c and C18 : 1ω7c. Phylogenetic analysis based on 16S rRNA gene sequences showed that both strains belong to the genus Nitratireductor , family Phyllobacteriaceae , class Alphaproteobacteria . Their closest neighbours were ‘ Nitratireductor lucknowense ’ DSM 24322 (96.3 and 96.5 % similarity, respectively) and Nitratireductor pacificus MCCC 1A01024T (96.2 and 96.3 % similarity, respectively). The DNA G+C contents of the two strains were 56.7 mol%. DNA–DNA hybridization between strain 2-2-12-1T and the two most closely related type strains revealed 57.3 and 52.3 % relatedness, respectively. Evidence from genotypic, chemotaxonomic and phenotypic data indicated that strains 2-2-12-1T and 2-2-12-2 represent a novel species of the genus Nitratireductor , for which the name Nitratireductor aestuarii sp. nov. is proposed. The type strain is 2-2-12-1T (=LMG 29090T=CGMCC 1.15320T).
-
-
-
Campylobacter ornithocola sp. nov., a novel member of the Campylobacter lari group isolated from wild bird faecal samples
More LessDuring a study on the prevalence and diversity of campylobacteria in wild birds faecal samples from the city of Valdivia (southern Chile) 17 Gram-stain-negative, curved-rod-shaped isolates, were initially identified as Campylobacter lari by PCR–RFLP. Further identification by 16S rRNA sequence analysis revealed that they formed a distinct group in the genus Campylobacter . This unique position was confirmed by the results of analysis of rpoB, atpA and cpn60 gene sequences. The average nucleotide identity between the representative strain WBE38T and the type strain of the most closely related taxon C. lari subsp. concheus (LMG 11760) was 90.8 %. The oxidase and urease activity of the novel isolates enabled them to be phenotypically differentiated from species of the genus Campylobacter with validly published names. Therefore, on the basis of phenotypic, genetic and genomic characterizations, the results of this study clearly indicate that these strains represent a novel species within the genus Campylobacter , for which the name Campylobacter ornithocola sp. nov. is proposed, with the type strain WBE38T (=CECT 9147T=LMG 29815T).
-
-
-
Pararhizobium antarcticum sp. nov., isolated from Antarctic water samples
More LessTwo bacterial strains were isolated from sediments and microbial mats of Kingfisher Pond, Antarctica and characterized in a taxonomic study using a polyphasic approach. Cells were strictly aerobic, Gram-stain-negative, rod-shaped, motile (+50 flagellum-specific genes present in the genome sequence; motility observed under microscope) and formed creamy white, half-transparent colonies. Growth occurred at 4 to 28 °C with an optimum at 20 °C, with 0–5.0 % (w/v) NaCl (optimum at 0–1.0 %) and at pH 4.0–11.0 (optimum pH 7.0–9.0). The major fatty acid was C18 : 1ω7c. The respiratory quinone was ubiquinone 10 (Q-10). The DNA G+C content was 60.7 mol %. The polar lipids were phosphatidylglycerol, phosphatidylethanolamine and phosphatidylmethanolamine in addition to three unidentified lipids, one unknown glycolipid and five unidentified phospholipids. Comparative analysis of 16S rRNA gene sequences showed highest sequence similarity (98.1 %) to Pararhizobium giardinii H152T, Pararhizobium herbae CCBAU 83011T, and ‘Pararhizobium polonicum’ F5.1. In silico average nucleotide identity (ANI) and genome-to-genome distance calculator (GGDC) showed 81.1 % identity (ANI) and 22.6 % identity (GGDC) to the closest relative, ‘P. polonicum’ F5.1. On the basis of phenotypic, phylogenetic, genomic and chemotaxonomic data, the two strains represent a novel species of the genus Pararhizobium , for which the name Pararhizobium antarcticum sp. nov. is proposed. The type strain is NAQVI 59T(=DSM 103442T=LMG 29675T).
-
-
-
Pseudomonas lactis sp. nov. and Pseudomonas paralactis sp. nov., isolated from bovine raw milk
Five strains, designated WS 4672T, WS 4998, WS 4992T, WS 4997 and WS 5000, isolated from bovine raw milk formed two individual groups in a phylogenetic analysis. The most similar species on the basis of 16S rRNA gene sequences were Pseudomonas azotoformans IAM 1603T, Pseudomonas gessardii CIP 105469T and Pseudomonas libanensis CIP 105460T showing 99.7–99.6 % similarity. Using rpoD gene sequences Pseudomonas veronii LMG 17761T (93.3 %) was most closely related to strain WS 4672T and Pseudomonas libanensis CIP 105460T to strain WS 4992T (93.3 %). The five strains could be differentiated from their closest relatives and from each other by phenotypic and chemotaxonomic characterization and ANIb values calculated from draft genome assemblies. ANIb values of strains WS 4992T and WS4671T to the closest relatives are lower than 90 %. The major cellular polar lipids of both strains are phosphatidylethanolamine, phosphatidylglycerol, a phospholipid and diphosphatidylglycerol, and their major quinone is Q-9. The DNA G+C content of strains WS 4992T and WS 4672T were 60.0 and 59.7 mol%, respectively. Based on these genotypic and phenotypic traits two novel species of the genus Pseudomonas are proposed: Pseudomonas lactis sp. nov. [with type strain WS 4992T (=DSM 29167T=LMG 28435T) and the additional strains WS 4997 and WS 5000], and Pseudomonas paralactis sp. nov. [with type strain WS 4672T (=DSM 29164T=LMG 28439T) and additional strain WS 4998].
-
-
-
Whole genome sequences reveal Vibrio hemicentroti Kim et al. 2013 as a later heterotypic synonym of Vibrio splendidus (Beijerinck 1900) Baumann et al. 1981
More LessThe synonymy between Vibrio hemicentroti Kim et al. 2013 and Vibrio splendidus (Beijerinck 1900) Baumann et al. 1981 was suggested after a recent multilocus sequence analysis of the Splendidus clade, which included the type strains of both species. To clarify their status, we have determined genomic indexes from whole genome sequences of strains V. hemicentroti CECT 8714T and V. splendidus NCCB 53037T. Average Nucleotide Identities of 96.0–96.7 % and an in silico DNA–DNA hybridization value of 70.2 %, as well as similarity levels of selected housekeeping gene sequences support the consideration of V. hemicentroti as a later heterotypic synonym of V. splendidus .
-
-
-
Oleiagrimonas citrea sp. nov., a marine bacterium isolated from tidal flat sediment and emended description of the genus Oleiagrimonas Fang et al. 2015 and Oleiagrimonas soli
More LessA Gram-stain-negative, aerobic, rod-shaped (1.4–3.6×0.4–0.6 µm) and motile marine bacterium, designated as MEBiC09124T, was isolated from tidal flat sediment of Suncheon Bay, South Korea. 16S rRNA gene sequence analysis revealed that strain MEBiC09124T showed high similarity to Oleiagrimonas soli 3.5XT (96.7 %). Growth was observed at 18–38 °C (optimum 30 °C), at pH 4.0–8.5 (optimum pH 7.5) and with 0–6 % (w/v) (optimum 2.5 %) NaCl. The predominant cellular fatty acids were iso-C15 : 0, iso-C16 : 0, iso-C17 : 0 and summed feature 9 (comprising 10-methyl C16 : 0 and/or iso-C17 : 1ω9c). The DNA G+C content was 66.1 mol%. The major respiratory quinone was Q-8. Biochemical characteristics such as production of acetoin and enzyme activities of trypsin, α-chymotrypsin and N-acetyl-β-glucosaminidase differentiated strain MEBiC09124T from O. soli 3.5XT. On the basis of data from this polyphasic taxonomic study, strain MEBiC09124T (=KCCM 43131T=JCM 30904T) is classified as the type strain of a novel species in the genus Oleiagrimonas , for which the name Oleiagrimonas citrea sp. nov. is proposed. Emended descriptions of the genus Oleiagrimonas and Oleiagrimonas soli are also given.
-
Volumes and issues
-
Volume 74 (2024)
-
Volume 73 (2023)
-
Volume 72 (2022 - 2023)
-
Volume 71 (2020 - 2021)
-
Volume 70 (2020)
-
Volume 69 (2019)
-
Volume 68 (2018)
-
Volume 67 (2017)
-
Volume 66 (2016)
-
Volume 65 (2015)
-
Volume 64 (2014)
-
Volume 63 (2013)
-
Volume 62 (2012)
-
Volume 61 (2011)
-
Volume 60 (2010)
-
Volume 59 (2009)
-
Volume 58 (2008)
-
Volume 57 (2007)
-
Volume 56 (2006)
-
Volume 55 (2005)
-
Volume 54 (2004)
-
Volume 53 (2003)
-
Volume 52 (2002)
-
Volume 51 (2001)
-
Volume 50 (2000)
-
Volume 49 (1999)
-
Volume 48 (1998)
-
Volume 47 (1997)
-
Volume 46 (1996)
-
Volume 45 (1995)
-
Volume 44 (1994)
-
Volume 43 (1993)
-
Volume 42 (1992)
-
Volume 41 (1991)
-
Volume 40 (1990)
-
Volume 39 (1989)
-
Volume 38 (1988)
-
Volume 37 (1987)
-
Volume 36 (1986)
-
Volume 35 (1985)
-
Volume 34 (1984)
-
Volume 33 (1983)
-
Volume 32 (1982)
-
Volume 31 (1981)
-
Volume 30 (1980)
-
Volume 29 (1979)
-
Volume 28 (1978)
-
Volume 27 (1977)
-
Volume 26 (1976)
-
Volume 25 (1975)
-
Volume 24 (1974)
-
Volume 23 (1973)
-
Volume 22 (1972)
-
Volume 21 (1971)
-
Volume 20 (1970)
-
Volume 19 (1969)
-
Volume 18 (1968)
-
Volume 17 (1967)
-
Volume 16 (1966)
-
Volume 15 (1965)
-
Volume 14 (1964)
-
Volume 13 (1963)
-
Volume 12 (1962)
-
Volume 11 (1961)
-
Volume 10 (1960)
-
Volume 9 (1959)
-
Volume 8 (1958)
-
Volume 7 (1957)
-
Volume 6 (1956)
-
Volume 5 (1955)
-
Volume 4 (1954)
-
Volume 3 (1953)
-
Volume 2 (1952)
-
Volume 1 (1951)