1887

Abstract

Four novel Gram-stain-positive, endospore-forming bacteria of the order were isolated from subsurface sediments sampled during International Ocean Discovery Program Expedition 347 to the Baltic Sea. One strain (59.4M) grew as an obligate heterotroph by aerobic respiration and anaerobically by fermentation. Optimum growth was observed with 0.5 % NaCl at 25 °C and pH 7.0–7.3. Analysis of 16S rRNA gene sequences of 59.4M revealed (92.3 % identity), Geosporobacter ferrireducens (92.2 %), (91.9 %) and (91.7 %) to be the closest relatives. On the basis of the results of phenotypic and genotypic analyses, we propose that strain 59.4M represents a novel species within a novel genus, gen. nov., sp. nov., with the type strain 59.4M (=DSM 102940=JCM 31103). Three other strains, 59.4F, 59.4B and 63.6F, were affiliated with the genus and grew as strictly anaerobic sulfate reducers. These strains additionally used thiosulfate, elemental sulfur, sulfite and DMSO as electron acceptors and hydrogen as an electron donor. Strains 59.4F and 59.4B had identical 16S rRNA gene sequences, which were most similar to those of (97.8 %), (97.3 %) and (97.3 %). Strain 63.6F was closely related to (97.7 %) (96.6 %) and (96.5 %). The similarity of 16S rRNA gene sequences of strains 59.4B and 63.6F was 96.6 %. We propose the new names sp. nov., incorporating strain 59.4F (=DSM 101562=JCM 31104) and the type strain 59.4B (=DSM 101608=JCM 31105), and sp. nov., with the type strain 63.6F (=DSM 101609=JCM 31106).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001883
2017-06-01
2024-12-05
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/6/1887.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001883&mimeType=html&fmt=ahah

References

  1. Biddle JF, Fitz-Gibbon S, Schuster SC, Brenchley JE, House CH. Metagenomic signatures of the Peru Margin subseafloor biosphere show a genetically distinct environment. Proc Natl Acad Sci USA 2008; 105:10583–10588 [View Article][PubMed]
    [Google Scholar]
  2. Jørgensen BB, Marshall IP. Slow microbial life in the seabed. Ann Rev Mar Sci 2016; 8:311–332 [View Article][PubMed]
    [Google Scholar]
  3. Klouche N, Fardeau ML, Lascourrèges JF, Cayol JL, Hacene H et al. Geosporobacter subterraneus gen. nov., sp. nov., a spore-forming bacterium isolated from a deep subsurface aquifer. Int J Syst Evol Microbiol 2007; 57:1757–1761 [View Article][PubMed]
    [Google Scholar]
  4. Zhilina TN, Zavarzina DG, Kolganova TV, Lysenko AM, Tourova TP. Alkaliphilus peptidofermentans sp. nov., a new alkaliphilic bacterial soda lake isolate capable of peptide fermentation and Fe(III) reduction. Microbiology 2009; 78:445–454 [View Article]
    [Google Scholar]
  5. Vandieken V, Knoblauch C, Jørgensen BB. Desulfotomaculum arcticum sp. nov., a novel spore-forming, moderately thermophilic, sulfate-reducing bacterium isolated from a permanently cold fjord sediment of Svalbard. Int J Syst Evol Microbiol 2006; 56:687–690 [View Article][PubMed]
    [Google Scholar]
  6. Hubert C, Loy A, Nickel M, Arnosti C, Baranyi C et al. A constant flux of diverse thermophilic bacteria into the cold Arctic seabed. Science 2009; 325:1541–1544 [View Article][PubMed]
    [Google Scholar]
  7. Müller AL, de Rezende JR, Hubert CR, Kjeldsen KU, Lagkouvardos I et al. Endospores of thermophilic bacteria as tracers of microbial dispersal by ocean currents. Isme J 2014; 8:1153–1165 [View Article][PubMed]
    [Google Scholar]
  8. Lomstein BA, Langerhuus AT, D'Hondt S, Jørgensen BB, Spivack AJ. Endospore abundance, microbial growth and necromass turnover in deep sub-seafloor sediment. Nature 2012; 484:101–104 [View Article][PubMed]
    [Google Scholar]
  9. Tschech A, Pfennig N. Growth yield increase linked to caffeate reduction in Acetobacterium woodii. Arch Microbiol 1984; 137:163–167 [View Article]
    [Google Scholar]
  10. Pfennig N. Rhodocyclus purpureus gen. nov. and sp. nov., a ring-shaped, vitamin B12-requiring member of the family Rhodospirillaceae. Int J Syst Bacteriol 1978; 28:283–288 [View Article]
    [Google Scholar]
  11. Widdel F, Bak F. Gram-negative mesophilic sulfate-reducing bacteria. In Balows A, Trüper HG, Dworkin M, Harder W, Schleifer KH. et al (editors) The Prokaryotes, 2nd ed. Berlin: Springer; 1992 pp. 3352–3378 [CrossRef]
    [Google Scholar]
  12. Kovacs N. Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 1956; 178:703 [View Article][PubMed]
    [Google Scholar]
  13. Grasshoff K, Kremling K, Ehrhardt M. Methods of Seawater Analysis Weinheim: Wiley-VCH Verlag; 1999 [CrossRef]
    [Google Scholar]
  14. Stookey LL. Ferrozine-a new spectrophotometric reagent for iron. Anal Chem 1970; 42:779–781 [View Article]
    [Google Scholar]
  15. Cord-Ruwisch R. A quick method for the determination of dissolved and precipitated sulfides in cultures of sulfate-reducing bacteria. J Microbiol Methods 1985; 4:33–36 [View Article]
    [Google Scholar]
  16. Blees J, Niemann H, Wenk CB, Zopfi J, Schubert CJ et al. Bacterial methanotrophs drive the formation of a seasonal anoxic benthic nepheloid layer in an alpine lake. Limnol Oceanogr 2014; 59:1410–1420 [View Article]
    [Google Scholar]
  17. Lane DJ. 16S/23S sequencing. In Stackebrandt E, Goodfellow M. (editors) Nucleic Acid Technique in Bacterial Systematics Chichester: Wiley; 1991 pp. 205–248
    [Google Scholar]
  18. Ludwig W, Strunk O, Westram R, Richter L, Meier H et al. ARB: a software environment for sequence data. Nucleic Acids Res 2004; 32:1363–1371 [View Article][PubMed]
    [Google Scholar]
  19. Sánchez-Andrea I, Stams AJ, Hedrich S, Ňancucheo I, Johnson DB. Desulfosporosinus acididurans sp. nov.: an acidophilic sulfate-reducing bacterium isolated from acidic sediments. Extremophiles 2015; 19:39–47 [View Article][PubMed]
    [Google Scholar]
  20. Takai K, Moser DP, Onstott TC, Spoelstra N, Pfiffner SM et al. Alkaliphilus transvaalensis gen. nov., sp. nov., an extremely alkaliphilic bacterium isolated from a deep south african gold mine. Int J Syst Evol Microbiol 2001; 51:1245–1256 [View Article][PubMed]
    [Google Scholar]
  21. Mayeux B, Fardeau ML, Bartoli-Joseph M, Casalot L, Vinsot A et al. Desulfosporosinus burensis sp. nov., a spore-forming, mesophilic, sulfate-reducing bacterium isolated from a deep clay environment. Int J Syst Evol Microbiol 2013; 63:593–598 [View Article][PubMed]
    [Google Scholar]
  22. Vatsurina A, Badrutdinova D, Schumann P, Spring S, Vainshtein M. Desulfosporosinus hippei sp. nov., a mesophilic sulfate-reducing bacterium isolated from permafrost. Int J Syst Evol Microbiol 2008; 58:1228–1232 [View Article][PubMed]
    [Google Scholar]
  23. Hong H, Kim SJ, Min UG, Lee YJ, Kim SG et al. Geosporobacter ferrireducens sp. nov., an anaerobic iron-reducing bacterium isolated from an oil-contaminated site. Antonie Van Leeuwenhoek 2015; 107:971–977 [View Article][PubMed]
    [Google Scholar]
  24. Ramamoorthy S, Sass H, Langner H, Schumann P, Kroppenstedt RM et al. Desulfosporosinus lacus sp. nov., a sulfate-reducing bacterium isolated from pristine freshwater lake sediments. Int J Syst Evol Microbiol 2006; 56:2729–2736 [View Article][PubMed]
    [Google Scholar]
  25. Robertson WJ, Bowman JP, Franzmann PD, Mee BJ. Desulfosporosinus meridiei sp. nov., a spore-forming sulfate-reducing bacterium isolated from gasolene-contaminated groundwater. Int J Syst Evol Microbiol 2001; 51:133–140 [View Article][PubMed]
    [Google Scholar]
/content/journal/ijsem/10.1099/ijsem.0.001883
Loading
/content/journal/ijsem/10.1099/ijsem.0.001883
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error