1887

Abstract

A novel alkaliphilic spore-forming bacterium was isolated from the benthic sediments of the highly mineralized steppe Lake Khilganta (Transbaikal Region, Russia). Cells of the strain, designated Х-07-2, were straight to slightly curved rods, Gram-stain-positive and motile. Strain Х-07-2 grew in the pH range from 7.0 to 10.7 (optimum pH 9.6–10.3). Growth was observed at 25–47 °C (optimum 30 °C) and at an NaCl concentration from 5 to 150 g l with an optimum at 40 g l. Strain Х-07-2 was a chemo-organoheterotroph able to reduce amorphous ferric hydroxide, Fe(III) citrate and elemental sulfur in the presence of yeast extract as the electron donor. It used tryptone, peptone and trypticase with Fe(III) citrate as the electron acceptor. The predominant fatty acids in cell walls were C16:1ω8, iso-C15:0, C14 : 0 3-OH and C16 : 0. The DNA G+C content was 32.6 mol%. 16S rRNA gene sequence analysis revealed that strain Х-07-2 was related most closely to members of the genus Alkaliphilus within the family Clostridiaceae . The closest relative was Alkaliphilus peptidifermentans Z-7036 (96.4 % similarity). On the basis of the genotypic, chemotaxonomic and phenotypic data, strain Х-07-2 represents a novel species in the genus Alkaliphilus , for which the name Alkaliphilus namsaraevii sp. nov. is proposed. The type strain is Х-07-2 (=VKM В-2746=DSM 26418).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001904
2017-06-20
2019-12-06
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/6/1990.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001904&mimeType=html&fmt=ahah

References

  1. Zavarzin GA, Zhilina TN, Kevbrin VV. The alkaliphilic microbial community and its functional diversity. Microbiologiya 1999;68:503–521
    [Google Scholar]
  2. Namsaraev Z, Gorlenko V, Buryukhaev S, Barkhutova D, Dambaev V et al. Water regime and changes of hydrochemical parameters of alkaline saline Lake Khilganta (South-Eastern Transbaikalia). Water Resources 2010;37:477–483[CrossRef]
    [Google Scholar]
  3. Takai K, Moser DP, Onstott TC, Spoelstra N, Pfiffner SM et al. Alkaliphilus transvaalensis gen. nov., sp. nov., an extremely alkaliphilic bacterium isolated from a deep South African gold mine. Int J Syst Evol Microbiol 2001;51:1245–1256 [CrossRef][PubMed]
    [Google Scholar]
  4. Cao X, Liu X, Dong X. Alkaliphilus crotonatoxidans sp. nov., a strictly anaerobic, crotonate-dismutating bacterium isolated from a methanogenic environment. Int J Syst Evol Microbiol 2003;53:971–975 [CrossRef][PubMed]
    [Google Scholar]
  5. Fisher E, Dawson AM, Polshyna G, Lisak J, Crable B et al. Transformation of inorganic and organic arsenic by Alkaliphilus oremlandii sp. nov. strain OhILAs. Ann N Y Acad Sci 2008;1125:230–241 [CrossRef][PubMed]
    [Google Scholar]
  6. Zhilina TN, Zavarzina DG, Kolganova TV, Lysenko AM, Tourova TP. Alkaliphilus peptidofermentans sp. nov., a new alkaliphilic bacterial soda lake isolate capable of peptide fermentation and Fe(III) reduction. Microbiology 2009;78:445–454 [CrossRef]
    [Google Scholar]
  7. Wu XY, Shi KL, Xu XW, Wu M, Oren A et al. Alkaliphilus halophilus sp. nov., a strictly anaerobic and halophilic bacterium isolated from a saline lake, and emended description of the genus Alkaliphilus. Int J Syst Evol Microbiol 2010;60:2898–2902 [CrossRef][PubMed]
    [Google Scholar]
  8. Ben Aissa F, Postec A, Erauso G, Payri C, Pelletier B et al. Characterization of Alkaliphilus hydrothermalis sp. nov., a novel alkaliphilic anaerobic bacterium, isolated from a carbonaceous chimney of the Prony hydrothermal field, New Caledonia. Extremophiles 2015;19:183–188 [CrossRef][PubMed]
    [Google Scholar]
  9. Ye Q, Roh Y, Carroll SL, Blair B, Zhou J et al. Alkaline anaerobic respiration: isolation and characterization of a novel alkaliphilic and metal-reducing bacterium. Appl Environ Microbiol 2004;70:5595–5602 [CrossRef][PubMed]
    [Google Scholar]
  10. Hwang C, Copeland A, Lucas S, Lapidus A, Barry K et al. Complete genome sequence of Alkaliphilus metalliredigens strain QYMF, an alkaliphilic and metal-reducing bacterium isolated from borax-contaminated leachate ponds. Genome Announc 2016;4:e01226-16 [CrossRef][PubMed]
    [Google Scholar]
  11. Roh Y, Chon CM, Moon JW. Metal reduction and biomineralization by an alkaliphilic metal-reducing bacterium, Alkaliphilus metalliredigens (QYMF). Geosciences J 2007;11:415–423 [CrossRef]
    [Google Scholar]
  12. Hungate RE. A roll tube method for cultivation of strict anaerobes. In Norris R, Ribbons RW. (editors) Methods in Microbiolologyvol. 13 New York: Academic Press; 1969; pp.117–132
    [Google Scholar]
  13. Reynolds ES. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol 1963;17:208–212 [CrossRef][PubMed]
    [Google Scholar]
  14. Smibert R, Krieg N. Phenotypic characterization. In Gerhardt P, Murray R, Wood W, Krieg N. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994; pp.607–654
    [Google Scholar]
  15. Cline JD. Spectrophotometric determination of hydrogen sulfide in natural waters. Limnol Oceanogr 1969;14:454–458 [CrossRef]
    [Google Scholar]
  16. Lovley DR, Phillips EJ. Availability of ferric iron for microbial reduction in bottom sediments of the freshwater tidal Potomac River. Appl Environ Microbiol 1986;52:751–757[PubMed]
    [Google Scholar]
  17. Lovley DR, Coates JD, Blunt-Harris EL, Phillips EJP, Woodward JC. Humic substances as electron acceptors for microbial respiration. Nature 1996;382:445–448 [CrossRef]
    [Google Scholar]
  18. Slobodkina GB, Panteleeva AN, Kostrikina NA, Kopitsyn DS, Bonch-Osmolovskaya EA et al. Tepidibacillus fermentans gen. nov., sp. nov.: a moderately thermophilic anaerobic and microaerophilic bacterium from an underground gas storage. Extremophiles 2013;17:833–839 [CrossRef][PubMed]
    [Google Scholar]
  19. Marmur J. A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 1961;3:208–218 [CrossRef]
    [Google Scholar]
  20. Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 1997;25:3389–3402 [CrossRef][PubMed]
    [Google Scholar]
  21. Benson DA, Boguski MS, Lipman DJ, Ostell J, Ouellette BF. GenBank. Nucleic Acids Res 1998;26:1–7 [CrossRef][PubMed]
    [Google Scholar]
  22. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013;30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  23. Marmur J, Doty P. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 1962;5:109–118 [CrossRef][PubMed]
    [Google Scholar]
  24. De Ley J, Cattoir H, Reynaerts A. The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 1970;12:133–142 [CrossRef][PubMed]
    [Google Scholar]
  25. Huss VA, Festl H, Schleifer KH. Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 1983;4:184–192 [CrossRef][PubMed]
    [Google Scholar]
  26. Sallam A, Steinbüchel A. Anaerobic and aerobic degradation of cyanophycin by the denitrifying bacterium Pseudomonas alcaligenes strain DIP1 and role of three other coisolates in a mixed bacterial consortium. Appl Environ Microbiol 2008;74:3434–3443 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001904
Loading
/content/journal/ijsem/10.1099/ijsem.0.001904
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error