1887

Abstract

Four rod–shaped and Gram-stain-negative bacterial strains, CCM 8647, CCM 8649, CCM 8643 and CCM 8648, were isolated from rock samples collected on James Ross Island, Antarctica. Extensive biotyping, fatty acid profiling, chemotaxonomy, 16S rRNA gene sequencing and whole-genome sequencing was applied to isolates to clarify their taxonomic position. Phylogenetic analysis based on 16S rRNA gene sequencing indicated that all four isolates belonged to the genus . Strains CCM 8649 and CCM 8647 were most closely related to OR362-8 (94.4 % 16S rRNA gene sequence similarity), strain CCM 8643 to DG7A (96.3 %) and strain CCM 8648 to VUG-A130 (96.3 %). The predominant fatty acids of CCM 8649 and CCM 8647 were summed feature 3 (Cω7/C ω6), Cω5 and iso-C, whereas those of CCM 8643 and CCM 8648 were summed feature 3 (Cω7/C ω6) and Cω5. The quinone systems contained exclusively menaquinone MK-7. The major polyamine was -homospermidine. All four strains contained the major polar lipid phosphatidylethanolamine. The G+C content of genomic DNA ranged from 60–63 mol%. Whole-genome sequencing data supported the finding that isolates represented distinct species of the genus . On the basis of the results obtained, three novel species are proposed for which the names sp. nov., sp. nov. and sp. nov. are suggested, with the type strains CCM 8649 (=LMG 29441=P5239), CCM 8643 (=LMG 29435=P3150) and CCM 8648 (=LMG 29440=P5086), respectively.

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2017-06-01
2020-11-24
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