1887

Abstract

Two bacterial strains, 2-2-12-1 and 2-2-12-2, were isolated from the estuary of the Jiulong River, south-east China. Cells were Gram-stain-negative, non-motile, short rods without flagella. Growth occurred at 25–45 °C, at pH 5.0–9.0 and with 0.5–2 % (w/v) NaCl. The bacteria were unable to reduce nitrate. The predominant fatty acids were C19 : 0 cyclo ω9c and C18 : 1ω7c. Phylogenetic analysis based on 16S rRNA gene sequences showed that both strains belong to the genus Nitratireductor , family Phyllobacteriaceae , class Alphaproteobacteria . Their closest neighbours were ‘ Nitratireductor lucknowense ’ DSM 24322 (96.3 and 96.5 % similarity, respectively) and Nitratireductor pacificus MCCC 1A01024 (96.2 and 96.3 % similarity, respectively). The DNA G+C contents of the two strains were 56.7 mol%. DNA–DNA hybridization between strain 2-2-12-1 and the two most closely related type strains revealed 57.3 and 52.3 % relatedness, respectively. Evidence from genotypic, chemotaxonomic and phenotypic data indicated that strains 2-2-12-1 and 2-2-12-2 represent a novel species of the genus Nitratireductor , for which the name Nitratireductor aestuarii sp. nov. is proposed. The type strain is 2-2-12-1 (=LMG 29090=CGMCC 1.15320).

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2017-06-22
2019-10-23
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References

  1. Labbé N, Parent S, Villemur R. Nitratireductor aquibiodomus gen. nov., sp. nov., a novel α-proteobacterium from the marine denitrification system of the Montreal Biodome (Canada). Int J Syst Evol Microbiol 2004;54:269–273 [CrossRef][PubMed]
    [Google Scholar]
  2. Kang HS, Yang HL, Lee SD. Nitratireductor kimnyeongensis sp. nov., isolated from seaweed. Int J Syst Evol Microbiol 2009;59:1036–1039 [CrossRef][PubMed]
    [Google Scholar]
  3. Kim KH, Roh SW, Chang HW, Nam YD, Yoon JH et al. Nitratireductor basaltis sp. nov., isolated from black beach sand. Int J Syst Evol Microbiol 2009;59:135–138 [CrossRef][PubMed]
    [Google Scholar]
  4. Lai Q, Yu Z, Yuan J, Sun F, Shao Z. Nitratireductor indicus sp. nov., isolated from deep-sea water. Int J Syst Evol Microbiol 2011;61:295–298 [CrossRef][PubMed]
    [Google Scholar]
  5. Lai Q, Yu Z, Wang J, Zhong H, Sun F et al. Nitratireductor pacificus sp. nov., isolated from a pyrene-degrading consortium. Int J Syst Evol Microbiol 2011;61:1386–1391 [CrossRef][PubMed]
    [Google Scholar]
  6. Jang GI, Hwang CY, Cho BC. Nitratireductor aquimarinus sp. nov., isolated from a culture of the diatom Skeletonema costatum, and emended description of the genus Nitratireductor. Int J Syst Evol Microbiol 2011;61:2676–2681 [CrossRef][PubMed]
    [Google Scholar]
  7. Manickam N, Pareek S, Kaur I, Singh NK, Mayilraj S. Nitratireductor lucknowense sp. nov., a novel bacterium isolated from a pesticide contaminated soil. Antonie van Leeuwenhoek 2012;101:125–131 [CrossRef]
    [Google Scholar]
  8. Pan XC, Geng S, Mei R, Wang YN, Cai H et al. Nitratireductor shengliensis sp. nov., isolated from an oil-polluted saline soil. Curr Microbiol 2014;69:561–566 [CrossRef][PubMed]
    [Google Scholar]
  9. Ou D, Chen B, Bai R, Song P, Lin H. Contamination of sulfonamide antibiotics and sulfamethazine-resistant Bacteria in the downstream and estuarine areas of Jiulong River in Southeast China. Environ Sci Pol Res 2015;22:12104–12113 [CrossRef]
    [Google Scholar]
  10. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994; pp.607–654
    [Google Scholar]
  11. Embley TM. The linear PCR reaction: a simple and robust method for sequencing amplified rRNA genes. Lett Appl Microbiol 1991;13:171–174 [CrossRef][PubMed]
    [Google Scholar]
  12. Frank JA, Reich CI, Sharma S, Weisbaum JS, Wilson BA et al. Critical evaluation of two primers commonly used for amplification of bacterial 16S rRNA genes. Appl Environ Microbiol 2008;74:2461–2470 [CrossRef][PubMed]
    [Google Scholar]
  13. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012;62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  14. Kumar S, Tamura K, Nei M. MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 2004;5:150–163 [CrossRef][PubMed]
    [Google Scholar]
  15. Dong X, Cai M. Systematic Identification Manual of Common Bacteria Beijing: Science Press; 2001
    [Google Scholar]
  16. Bauer AW, Kirby WMM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Amer J Clin Pathol 1966;45:493–496
    [Google Scholar]
  17. Fraser SL, Jorgensen JH. Reappraisal of the antimicrobial susceptibilities of Chryseobacterium and Flavobacterium species and methods for reliable susceptibility testing. Antimicrob Agents Chemother 1997;41:2738–2741[PubMed]
    [Google Scholar]
  18. Andrews JM. BSAC standardized disc susceptibility testing method (version 7). JAntimicrob Chemoth 2008;62:256–278 [CrossRef]
    [Google Scholar]
  19. Collins MD. Analysis of isoprenoid quinones. In Gottschalk G. (editor) Methods in Microbiology London: Academic Press; 1985; pp.329–363
    [Google Scholar]
  20. Komagata K, Suzuki K I. Lipid and cell-wall analysis in bacterial systematics. In Colwell RR, Grigorova R. (editors) Methods in Microbiology London: Academic Press; 1987; pp.161–208
    [Google Scholar]
  21. Kates M. Lipid extraction procedures. In Burdun RH, van Knippenberg PH. (editors) Laboratory Techniques in Biochemistry and Molecular Biology Amsterdam: Elsevier; 1986; pp.350–351
    [Google Scholar]
  22. Marmur J. A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 1961;3:208–218 [CrossRef]
    [Google Scholar]
  23. Johnson JL. Similarity analysis of DNAs. In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994; pp.655–682
    [Google Scholar]
  24. Mandel M, Igambi L, Bergendahl J, Dodson ML, Scheltgen E. Correlation of melting temperature and cesium chloride buoyant density of bacterial deoxyribonucleic acid. J Bacteriol 1970;101:333–338[PubMed]
    [Google Scholar]
  25. Coram NJ, Rawlings DE. Molecular relationship between two groups of the genus Leptospirillum and the finding that Leptospirillum ferriphilum sp. nov. dominates South African commercial biooxidation tanks that operate at 40 °C. Appl Environ Microbiol 2002;68:838–845 [CrossRef][PubMed]
    [Google Scholar]
  26. Tønjum T, Welty DB, Jantzen E, Small PL. Differentiation of Mycobacterium ulcerans, M. marinum, and M. Haemophilum: mapping of their relationships to M. tuberculosis by fatty acid profile analysis, DNA-DNA hybridization, and 16S rRNA gene sequence analysis. J Clin Microbiol 1998;36:918–925[PubMed]
    [Google Scholar]
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