1887

Abstract

[Lewin, 1969] was reclassified as NBRC 15981 was reclassified herein by using a polyphasic taxonomic approach. Cells of the strain were strictly aerobic, Gram-stain-negative, slender rods, which were motile by gliding. The major respiratory quinone was menaquinone-7 and the predominant (>5 %) cellular fatty acids were iso-C, iso-G-C, Cω7 and iso-C 3-OH. The polar lipid pattern indicated the presence of a phosphatidylethanolamine, several unidentified aminolipids, glycolipids and five unidentified polar lipids. The G+C content of the genomic DNA was 35.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain NBRC 15981 clustered with members of the genus in the family of the phylum . Levels of DNA–DNA relatedness were less than 16 % between strain NBRC 15981 and the two closely related species, NBRC 15983 and NBRC 15989. Strain NBRC 15981 could be differentiated from these type strains in the genus based on the polar lipid pattern and the activity of α-chymotrypsin, as well as by α-glucosidase and β-glucosidase activity. On the basis of these results, NBRC 15981 is proposed as representing a novel species of the genus , named sp. nov. The type strain is JK11 (=NBRC 15981=NCIMB 1429).

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2017-06-01
2020-01-22
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