1887

Abstract

A Gram-stain-negative, aerobic, non-motile and ovoid or rod-shaped bacterial strain, designated OITF-11, was isolated from a tidal flat in Oido, an island of South Korea, and subjected to a polyphasic taxonomic study. Strain OITF-11 grew optimally at 25 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. The phylogenetic trees based on 16S rRNA gene sequences showed that strain OITF-11 belonged to the genus Polaribacter . Strain OITF-11 exhibited 16S rRNA gene sequence similarity values of 97.4–98.1 % to Polaribacter haliotis RA4-7, Polaribacter atrinae KACC 17473, Polaribacter dokdonensis DSW-5 and Polaribacter marinaquae KCTC 42664, and of 94.1–96.9 % to the type strains of the other Polaribacter species. Strain OITF-11 contained menaquinone MK-6 as the predominant menaquinone and iso-C15 : 0, iso-C15 : 1 G, iso-C15 : 0 3-OH, iso-C17 : 0 3-OH and C15 : 1ω6c as the major fatty acids. The major polar lipids detected in strain OITF-11 were phosphatidylethanolamine, phosphatidylmonomethylethanolamine and one unidentified lipid. The DNA G+C content of strain OITF-11 was 32.2 mol% and its DNA–DNA relatedness values with the type strains of P. haliotis , P. atrinae , P. dokdonensis and P. marinaquae were 14–33 %. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain OITF-11 is separated from recognized species of the genus Polaribacter . On the basis of the data presented, strain OITF-11 is considered to represent a novel species of the genus Polaribacter , for which the name Polaribacter litorisediminis sp. nov. is proposed. The type strain is OITF-11 (=KCTC 52500=NBRC 112457).

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2017-06-30
2019-10-15
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