1887

Abstract

A novel sulfur-reducing bacterium, strain K6013, was isolated from a sulfide sample collected at a depth of 2771 m from a high-temperature hydrothermal vent in the Indian Ocean. Cells were Gram-stain-negative, anaerobic, motile rods (0.9–2.2×0.4–0.6 µm). The strain grew at NaCl concentrations ranging from 1 to 4.5 % (w/v) (optimum 2.5 %), at pH 5 to 8 (optimum pH 6), and at temperatures between 40 and 75 °C (optimum 65 °C). K6013 was an obligate chemolithoautotroph, using thiosulfate, sulfur and nitrate as terminal electron acceptors in the presence of H but not sulfate, sulfite nor nitrite. The major cellular fatty acids were C (17.4 %), Cω7/Cω6 (ummed feature 8, 37.91 %), C (18.29 %) and C 3-OH/iso-CI (summed feature 2, 8.56 %). The DNA G+C content was 38.2 mol%. The results of phylogenetic 16S rRNA gene sequence analyses indicated that K6013 represents a member of the genus within the class , with highest sequence similarity of 96.93 % to SL22. On the basis of genotypic and phenotypic data, K6013 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed, with the type strain K6013 (=DSM 101677=MCCC 1A01868).

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2017-06-01
2020-01-17
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