- Volume 67, Issue 3, 2017

‘ Streptomyces caelicus ’ DSM 40835 was first reported as the producer of the antibiotic griselimycin by some coworkers of Rhone Poulenc in 1971. The project on isolation of the antibiotic compound was stopped because of the bad solubility and selectivity of the compound towards Mycobacteria. At Sanofi-Aventis, Germany, the project was re-evaluated in 2007 and the gene cluster of griselimycin could be identified, characterized and was patented in 2013. At this time, ‘ S. caelicus ’ was an invalid name. During the strain characterization work, it was found that ' S. caelicus ' belongs to the group of species of the genus Streptomyces which show an unusual heterogeneity of the 16S rRNA gene sequences. However, high 16S rRNA gene sequence similarities to Streptomyces muensis JCM 17576T and Streptomyces canchipurensis JCM 17575T were obvious. Here, we present a comparative description of ' Streptomyces caelicus ’ DS 9461 (=DSM 40835=NCCB 100592) with S. muensis and S. canchipurensis by use of a polyphasic taxonomy approach and additional comparison of some housekeeping genes by multilocus sequence analysis (MLSA). An emended description of Streptomyces muensis is provided as a result of this work.

An aerobic, mildly acidophilic actinobacterium was isolated from the Ochre Beds bog in Kootenay National Park, Canada. Cells of isolate OB1T were Gram-stain-positive, non-motile, pink- to purple-pigmented filaments. The pH range for growth was pH 3.5–6.5 (optimum pH 5.5), and the temperature range was 13–30°C. The major cellular fatty acids were i-C16 : 0 (28.5 %), i-C15 : 0 (14.6 %) and ai-C15 : 0 (14.3 %), and the major polar lipid was phosphohexose. The major quinone was menaquinone-11 (MK-11), and the peptidoglycan type was A1γ. The DNA G+C content was 70.2 %. Along with growth on complex media including yeast extract, proteose peptone, casamino acids and tryptic soy broth, growth occured on mono- and disaccharides (glucose, sucrose, galactose and xylose) and polysaccharides (starch, gellan, pectin, xylan and alginate). Anaerobic growth was not observed. The cells did not fix atmospheric nitrogen. On the basis of comparative 16S rRNA gene sequence analysis, this isolate belonged to the family Actinospicaceae , in the suborder Catenulisporineae of the order Actinomycetales . The most closely related species was Actinospica robiniae . However, the 16S rRNA gene sequence identity to this bacterium was low (92.8 %) and there were several chemotaxonomic differences from this species. We therefore propose a novel genus and species, Actinocrinis puniceicyclus gen. nov., sp. nov., with strain OB1T (=DSM 45618T=ATCC BAA-2771T) as the type strain.

A novel actinobacterial strain, designated YIM A1136T, was isolated from a soil sample collected from a karst cave in Xingyi county, Guizhou province, south-western China. The taxonomic position of the strain was investigated using a polyphasic approach. Cells of the strain were aerobic, Gram-staining-positive and rod-shaped. Colonies of the strain were circular, convex, opaque and yellowish-white in colour. On the basis of 16S rRNA gene sequence analysis, strain YIM A1136T was most closely related to the type strains Nocardioides ganghwensis JC2055T (98.3 % sequence similarity), Nocardioides exalbidus RC825T (98.2 %), Nocardioides alpinus Cr7-14T (98.2 %), Nocardioides hwasunensis HFW-21T (98.0 %), Nocardioides oleivorans DSM 16090T (97.9 %) and Nocardioides furvisabuli SBS-26T (97.8 %) and is therefore considered to represent a member of the genus Nocardioides . DNA–DNA hybridization values between strain YIM A1136T and related type strains of the genus Nocardioides were less than 70 %. ll-Diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan. The whole-cell sugars were galactose, glucose, mannose, rhamnose and ribose. The major isoprenoid quinone was MK-8(H4), while the major fatty acids (>10 %) were C16 : 0, summed feature 3 and summed feature 8. The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and two unidentified phospholipids. The genomic DNA G+C content was 71.4 mol%. On the basis of phenotypic, genotypic and phylogenetic data, strain YIM A1136T merits representation of a novel species of the genus Nocardioides , for which the name Nocardioides cavernae sp. nov. is proposed. The type strain is YIM A1136T (=KCTC 39551T=DSM 29950T).

Two Gram-stain-variable, non-motile, catalase-positive and cytochrome c oxidase-negative bacteria, designated AK20-18T and AM20-54, were isolated from forest soil samples collected in the Italian Alps. Growth occurred at a temperature range of 5–30 °C, at pH 6–9 and in the presence of 0–5 % (w/v) NaCl. The 16S rRNA gene sequence similarity between strains AK20-18T and AM20-54 was 100 %. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain AK20-18T had highest 16S rRNA gene sequence similarity with the type strain of Arthrobacter psychrochitiniphilus (96.9 %). The cell-wall peptidoglycan structure of strain AK20-18T was of the type A3alpha l-Lys–l-Thr–l-Ala2 (A11.27). The whole-cell sugars were galactose, ribose and lesser amounts of mannose. The major respiratory quinone of the two strains was menaquinone 9(H2) [MK-9(H2)], whereas MK-10(H2) was a minor component. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and unknown glycolipids. The major cellular fatty acids were anteiso-C15 : 0, iso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0. The genomic DNA G+C content was 59.9 mol%. Combined data of phylogenetic, phenotypic and chemotaxonomic analyses demonstrated that strains AK20-18T and AM20-54 represent a novel genus and species, for which the name Psychromicrobium silvestre gen. nov., sp. nov. is proposed. The type strain of Psychromicrobium silvestregen. nov., sp. nov. is AK20-18T (=DSM 102047T=LMG 29369T).

A novel halophilic actinomycete strain, designated TRM 46074T, was isolated from Aiding salt lake in Turpan Basin, north-west China. Strain TRM 46074T was aerobic, Gram-stain-positive and grew optimally with 12 % (w/v) NaCl. The strain was observed to produce sparse aerial mycelium with long chains of spores that were non-motile and oval or spherical in shape with a rough or smooth surface; substrate mycelium was branched and well developed. The cell wall was determined to contain meso-diaminopimelic acid; the diagnostic whole-cell sugars were galactose, arabinose and ribose. The diagnostic polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol and phosphatidylinositol. The predominant menaquinones were MK-9(H4), MK-10(H8) and MK-10(H4). The G+C content of the DNA was 70.9 mol%. The major fatty acids were anteiso-C17 : 0, iso-C15 : 0, iso-C16 : 0 and iso-C17 : 0. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain TRM 46074T belongs to the genus Saccharopolyspora . Strain TRM 46074T had 16S rRNA gene sequence similarity of 99.6 % with the closest described species, Saccharopolyspora ghardaiensis H53T. DNA–DNA hybridization between strain TRM 46074T and Saccharopolyspora ghardaiensis H53T showed 58.6 % relatedness. On the basis of the evidence from a polyphasic study, strain TRM 46074T is considered to represent a novel species in the genus Saccharopolyspora , for which the name Saccharopolyspora aidingensis sp. nov. is proposed. The type strain is TRM 46074T (=CCTCC AA 2012014T=JCM 30185T).

A Gram-stain-positive, aerobic bacterial strain, designated SL15-1T, was isolated from desert soil which was sampled from the rhizosphere of Ammopiptanthus mongolicus, Hangjin Banner, Ordos, Inner Mongolia, northern China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain SL15-1T was clustered with Kribbella strains, sharing the highest similarity of 16S rRNA gene sequence (96.97 %) with Kribbella sandramycini DSM 15626T. Strain SL15-1T contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, an unknown phospholipid, an unknown lipid and two unknown aminophospholipids as the major polar lipids. MK-9(H4) was the predominant menaquinone, while anteiso-C15 : 0, iso-C16 : 0, C17 : 1ω8c and iso-C14 : 0 were the major cellular fatty acids. Its genomic DNA G+C content was 65.3 mol%. The results of physiological and biochemical tests allowed the discrimination of strain SL15-1T from its phylogenetic relatives. Kribbella deserti sp. nov. is therefore proposed with strain SL15-1T (=CGMCC 1.15906T=KCTC 39825T) as the type strain.

A bacterial strain, designated 5GHs33-3T, was isolated from greenhouse soil collected from Yongin region, Gyeonggi province, South Korea. The strain was an aerobic, Gram-stain-positive, flagellated, rod-shaped bacterium. Strain 5GHs33-3T grew at 4–37 °C (optimally at 28–30 °C), pH 6.0–10.0 (optimally at pH 7.0) and with 0–7 % (w/v) NaCl (optimally with 0 %). The 16S rRNA gene sequence analysis revealed that strain 5GHs33-3T had high sequence similarity with Actinotalea suaedae EGI 60002T (98.4 %), Actinotalea ferrariae CF5-4T (96.4 %) and Actinotalea fermentans DSM 3133T (96.2 %), and less than 95.5 % sequence similarity against all the other species with validly published names. The phylogenetic tree revealed that strain 5GHs33-3T formed a robust independent monophyletic line with Actinotalea suaedae EGI 60002T. The predominant fatty acids of strain 5GHs33-3T were anteiso-C15 : 0 and iso-C14 : 0. The only quinone was MK-8(H4). Polar lipids were diphosphatidylglycerol, phosphatidylinositol, an unknown phosphoglycolipid and unknown lipids. The peptidoglycan type was A4β, with ornithine as the diagnostic diamino acid and an interpeptide bridge comprising l-Glu. The DNA G+C content is 69.0 mol%. Based on phylogenetic evidence and the results of phenotypic, genotypic and chemotaxonomic analyses, strain 5GHs33-3T represents a novel species of a new genus of the family Cellulomonadaceae , for which the name Pseudactinotalea terrae gen. nov., sp. nov. is proposed. The type strain of the type species is 5GHs33-3T (=KACC 16542T=NBRC 111006T). We also propose the reclassification of Actinotalea suaedae as Pseudactinotalea suaedae comb. nov. (type strain EGI 60002T=JCM 19624T=KACC 17839T=KCTC 29256T).

Strain JBM2-3T, a pale-yellow-coloured, aerobic, catalase-negative, oxidase-positive and Gram-stain-negative bacterium, was isolated from wet soil. The isolate grew aerobically at 25–30 °C (optimum 25 °C), pH 6.0–8.0 (optimum pH 7.0) and in the presence of 0–0.5 % (w/v) NaCl (optimum 0 % NaCl). Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain JBM2-3T belonged to the genus Spirosoma , with a sequence similarity of 96.2 % with Spirosoma panaciterrae Gsoil 1519T. The strain showed the typical chemotaxonomic characteristics of the genus Spirosoma , with the presence of menaquinone 7 as the respiratory quinone; the major fatty acids were summed feature 3 (composed of C16: 1ω6c /ω7c), C16: 1ω5c and iso-C15: 0. The DNA G+C content of strain JBM2-3T was 47.4 mol%. The polar lipid profile contained major amounts of phosphatidylethanolamine and aminophospholipids. On the basis of its phenotypic and genotypic properties, and phylogenetic distinctiveness, strain JBM2-3T should be classified as a representative of a novel species in the genus Spirosoma , for which the name Spirosoma swuense sp. nov. is proposed. The type strain is JBM2-3T (=KCTC 52176T=JCM 31298T).

A Gram-stain-negative, smooth, bright-yellow-pigmented, rod-shaped bacterial strain, slightly motile by gliding, catalase- and oxidase-positive and aerobic, but growing weakly under anaerobic conditions, was isolated from the rhizosphere of the flower mugunghwa (Hibiscus syriacus L.) located in Kyung Hee University, Yongin, Gyeonggi, South Korea. The strain named THG-HG1.4T grew at 15–35 °C, pH 6.5–9.0 and in the presence of 0–2.5 % (w/v) NaCl. The phylogenetic analysis based on 16S rRNA gene sequence showed that strain THG-HG1.4T was most closely related to Flavobacterium gyeonganense HME7524T (98.83 %) and Flavobacterium arsenitoxidans S2-3HT (97.28 %). The DNA G+C content of strain THG-HG1.4T was 41.2 mol%. In DNA–DNA hybridization, the DNA–DNA relatedness between strain THG-HG1.4T and its closest phylogenetic neighbour was below 64.1 %. The predominant isoprenoid quinone detected in strain THG-HG1.4T was menaquinone-6 (MK-6). The major polar lipids were found to be phosphatidylethanolamine, three unidentified lipids, two unidentified glycolipids and an unidentified aminolipid. The major fatty acids were identified as iso-C15 : 0, iso-C15 : 0 3-OH, C16 : 0, iso-C17 : 0 3-OH and summed feature 3. Thus, based on the report of the phenotypic, genotypic and phylogenetic characterization of strain THG-HG1.4T, it has been concluded that the novel isolate represents a novel species of the genus Flavobacterium. Flavobacterium hibisci sp. nov. is proposed, with THG-HG1.4T (=KACC 18852T=CCTCC AB 2016178T) as the type strain.

A Gram-stain-negative, aerobic, yellow-pigmented, flexirubin-producing, rod-shaped and endophytic bacterium, designated strain CC-YTH209T, was isolated from a maize leaf and subjected to a taxonomic study. Strain CC-YTH209T was found to grow at 15–40 °C (optimal 30 °C), at pH 6.0–8.0 (optimal pH 7.0) and in the presence of 0–2 % (optimal 1 %) (w/v) NaCl. On the basis of 16S rRNA gene sequence analysis, strain CC-YTH209T appeared to belong to the genus Chryseobacterium within the class Flavobacteriia , and was closely related to Chryseobacterium rigui CJ16T (97.5 % similarity) and Chryseobacterium taeanense PHA3-4T (96.9 %). The level of DNA–DNA relatedness between strain CC-YTH209T and Chryseobacterium rigui CJ16T was 14.4 % (reciprocal, 13.0 %). Phylogenetic analyses based on 16S rRNA genes revealed a distinct taxonomic position attained by strain CC-YTH209T within the clade that accommodated Chryseobacterium species. The DNA G+C content was 37.2 mol%. Strain CC-YTH209T contained menaquinone MK-6 as the predominant respiratory quinone and sym-homospermidine as the major polyamine. The major cellular fatty acids of the isolate were iso-C15 : 0, iso-C17 : 0 3-OH and C16 : 1 ω6c/C16  : 1 ω7c. The polar lipid profile comprised phosphatidylethanolamine and five aminolipids, three lipids, one glycolipid, one aminophospholipid and one phospholipid, which are presently uncharacterized. On the basis of evidence presented in this study, strain CC-YTH209T is considered to represent a novel species of the genus Chryseobacterium , for which the name Chryseobacterium endophyticum sp. nov. is proposed. The type strain is CC-YTH209T (=BCRC 80938T=JCM 31226T).

A Gram-stain-negative, non-motile, aerobic and rod-shaped bacterium, designated OITF-20T, was isolated from the tidal flat in Oido, an island of South Korea. The novel strain grew optimally at 25 °C, at pH 7.0–8.0 and in the presence of 1.0–2.0 % (w/v) NaCl. In the neighbour-joining phylogenetic tree based on 16S rRNA gene sequences, strain OITF-20T fell within the clade comprising the type strains of species of the genus Lutibacter . Strain OITF-20T exhibited 16S rRNA gene sequence similarity values of 97.8, 97.6 and 97.6 % to the type strains of Lutibacter crassostreae , Lutibacter flavus and Lutibacter litoralis , respectively, of 94.9–96.8 % to the type strains of the other species of the genus Lutibacter and of less than 93.4 % to the type strains of the other recognized species. Strain OITF-20T contained MK-6 as the predominant menaquinone and iso-C15 : 0 3-OH, C15 : 1ω6c and iso-C15 : 0 as the major fatty acids. The major polar lipids of strain OITF-20T were phosphatidylethanolamine and one unidentified lipid. The DNA G+C content of strain OITF-20T was 31.1 mol% and its mean DNA–DNA relatedness values with the type strains of L. crassostreae , L. flavus and L. litoralis were 17–27 %. The differential phenotypic properties, together with phylogenetic and genetic distinctiveness, revealed that strain OITF-20T is separated from other recognized species of the genus Lutibacter . On the basis of the data presented, strain OITF-20T is considered to represent a novel species of the genus Lutibacter , for which the name Lutibacter litorisediminis sp. nov. is proposed. The type strain is OITF-20T (=KCTC 52501T=NBRC 112458T).

A Gram-stain-negative, aerobic, rod-shaped, non-motile and pink-pigmented bacterial strain, designated 16F7GT, was isolated from river water. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 16F7GT belongs to the genus Hymenobacter . The 16S rRNA gene sequence similarity to members of the genus Hymenobacter ranged from 90.5 to 97.4 %, and the most closely related strains were Hymenobacter rigui WPCB131T (97.4 %) and Hymenobacter xinjiangensis X2-1gT (97.3 %). Strain 16F7GT had <70 % DNA–DNA relatedness with H. rigui (32.8±7.8 %) and H. xinjiangensis (30.2±6.2 %), indicating that it represents a novel genospecies. Cells were catalase- and oxidase-positive. The genomic DNA G+C content was 56.6 mol%. The major fatty acids were summed feature 4 (C17 : 1iso I/C17 : 1 anteiso B; 19.8 %), summed feature 3 (C16 : 1 ω7c/C16  : 1 ω6c; 18.4 %), C15 : 0 iso (17.0 %), C16 : 1 ω5c (11.8 %) and C15 : 0 anteiso (9.8 %). The major polar lipid was phosphatidylethanolamine and the predominant respiratory quinone was menaquinone 7 (MK-7). Based on the phylogenetic, phenotypic, genotypic and chemotaxonomic analyses, it is concluded that strain 16F7GT represents a novel species within the genus Hymenobacter , for which the name Hymenobacter seoulensis sp. nov. is proposed. The type strain is 16F7GT (=KCTC 52197T=JCM 31655T).

The Gram-stain-negative, yellow-pigmented, rod-shaped bacterial strain GSE06T, isolated from the surface-sterilized root of a cucumber plant grown in a field in Gunsan, Korea, was characterized by not only cultural and morphological features but also physiological, biochemical and molecular analyses. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain GSE06T was most closely related to species of the genus Chryseobacterium . Furthermore, strain GSE06T exhibited the highest sequence similarities with the type strains Chryseobacterium indologenes ATCC 29897T (98.9 %), Chryseobacterium gleum ATCC 35910T (98.8 %), Chryseobacterium arthrosphaerae CC-VM-7T (98.7 %), Chryseobacterium contaminans C26T (98.5 %), Chryseobacterium artocarpi UTM-3T (98.3 %), and Chryseobacterium gallinarum 100T (97.9 %). Average nucleotide identity values between genome sequences of strain GSE06T and the above-mentioned reference strains ranged from 81.2 to 86.9 %, which were lower than the threshold of 95 % (corresponding to a DNA–DNA reassociation value of 70 %). The DNA G+C content of strain GSE06T was 36.1 mol%; the predominant respiratory quinone of the strain was MK-6. The major fatty acids were iso-C15 : 0, summed feature 9 (iso-C17 : 1ω9c), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and iso-C17 : 0 3-OH. The major polar lipids were phosphatidylethanolamine, three aminolipids, one aminophospholipid, four glycolipids and one unidentified lipid. These results of phenotypic and genotypic characteristics could differentiate strain GSE06T from closely related type strains belonging to the genus Chryseobacterium . Thus, strain GSE06T is proposed as a representative of a novel species in the genus Chryseobacterium, Chryseobacterium cucumeris sp. nov. The type strain is GSE06T (=KACC 18798T=JCM 31422T).

During the study of hydrocarbon-degrading bacteria in the oil-contaminated soil of Biratnagar, Morang, Nepal, a yellow-coloured, Gram-staining-negative, aerobic, non-motile, and rod-shaped bacterium, designated strain C-5-3T, was isolated. This strain was characterized taxonomically by a polyphasic approach. Based on the 16S rRNA gene sequence analysis, strain C-5-3T belonged to the genus Chryseobacterium and was closely related to Chryseobacterium profundimaris DY46T (98.19 % sequence similarity), Chryseobacterium takakiae AG1-2T (98.15 % sequence similarity), Chryseobacterium taiwanense BCRC 17412T (98.14 % sequence similarity), Chryseobacterium camelliae THG C4-1T (97.73 % sequence similarity) and Chryseobacterium hispalense DSM 25574T (97.60 % sequence similarity). The predominant respiratory quinone was menaquinone-6, and phosphatidylethanolamine was the major polar lipid. The predominant fatty acids of strain C-5-3T were iso-C15 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C17 : 0 3-OH and summed feature 9 (iso- C17 : 1ω9c and/or C16 : 0 10-methyl). The genomic DNA G+C content of this novel strain was 38.6 mol%. The DNA–DNA relatedness between strain C-5-3T and Chryseobacterium profundimaris JCM 19801T, C. takakiae DSM 26898T, C. taiwanense KACC 13400T, C. camelliae KACC 16985T and C. hispalense DSM 25574T was 53.3, 42.7, 47.3, 33.0 and 28.0 %, respectively. The morphological, physiological, chemotaxonomic and phylogenetic analyses clearly distinguished this strain from its closest phylogenetic neighbours. Thus, strain C-5-3T represents a novel species of the genus Chryseobacterium , for which the name Chryseobacterium nepalense sp. nov. is proposed. The type strain is C-5-3T (=KEMB 9005-411T=KACC 18907T=JCM 31469T).

A Gram-stain-negative, non-spore-forming, rod-shaped, non-motile bacterium, named wh15T, was isolated from the waste water of a municipal wastewater treatment plant in Wuhu, PR China. The taxonomic status of the strain was characterized using a polyphasic taxonomic approach. Growth occurred at 15–45 °C (optimum, 25–40 °C), at pH 5.0–8.0 (optimum, pH 7.0) and with 0–4 % NaCl (optimum, 1–2 %). Phylogenetic analyses based on 16S rRNA gene sequences revealed that wh15T represented a member of genus Flavobacterium and shared the highest similarity with Flavobacterium ummariense DS-12T (93.93 %), Flavobacterium ceti 454–2T (93.71 %) and Flavobacterium marinum SW105T (93.63 %). The major respiratory quinone of wh15T was MK-6 and major fatty acids were iso-C15 : 0, iso-C15 : 0 3-OH, iso-C17 : 0 3-OH, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and summed feature 9 (iso-C17 : 1ω9c and/or C16 : 0 10-methyl). The DNA G+C content of wh15T was 32.3 mol%. Phenotypic, phylogenetic and chemotaxonomic evidence indicated that wh15T represents a novel species of the genus Flavobacterium , the name Flavobacterium cloacae is proposed. The type strain is wh15T (=CCTCC AB 2015420T=JCM 31173T).

A Gram-staining-negative, aerobic, non-motile and yellow-pigmented bacterium, designated strain SM1504T, was isolated from Arctic seawater. It hydrolysed aesculin and gelatin but did not reduce nitrate to nitrite. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain SM1504T constituted a distinct phylogenetic line within the family Cytophagaceae and was closely related to species of the genera Lacihabitans , Emticicia , Fluviimonas and Leadbetterella , with respect to which low sequence similarities between 88.9 and 91.6 % were observed. The major fatty acids of strain SM1504T were summed feature 3 (comprising C16 : 1ω7c and/or iso-C15 : 0 2-OH) and iso-C15 : 0. The predominant polar lipids of strain SM1504T were phosphatidylethanolamine and one unidentified lipid. The only respiratory quinone detected in strain SM1504T was MK7. The DNA G+C content of strain SM1504T was 40.8 mol%. On the basis of the phylogenetic, chemotaxonomic and phenotypic characterization in this study, strain SM1504T is considered to represent a novel species in a new genus of the family Cytophagaceae , for which the name Arcticibacterium luteifluviistationis gen. nov., sp. nov. is proposed. The type strain is SM1504T (=KCTC 42716T=CCTCC AB 2015348T).

A yellowish-orange-pigmented marine bacterium, designated MEBiC08714T was isolated from a sea urchin Strongylocentrotus intermedius collected at the west edge of the East Sea of Korea. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that MEBiC08714T was affiliated with the genus Echinicola and that the strain was most closely related to Echinicola vietnamensis KCTC 12713T (96.9 %), followed by ‘ Echinicola shivajiensis ’ JCM 17847 (96.3 %), Echinicola jeungdonensis KCTC 23122T (96.1 %), and Echinicola pacifica KCTC 12368T (95.0 %). Cells were strictly aerobic, Gram-stain-negative, rod shaped, flexirubin-type pigments-negative and motile by gliding. Growth was observed at 20–35 °C (optimum 25 °C), at pH 6–11 (optimum pH 7.0), and with 0–13 % NaCl (optimum 2 %). This strain was able to hydrolyze agar and starch. The polar lipids of MEBiC08714T contained phosphatidylethanolamine, an unidentified phospholipid, and four unidentified lipids. The major fatty acids were iso-C15 : 0 (27.5 %), iso-C17 : 0 3-OH (11.5 %), anteiso-C15 : 0 (5.2 %), summed feature 3 (C16:1ω7c and/or C16:1ω6c, 20.3 %) and summed feature 9 (iso-C17 : 1ω9c and/or10-methyl C16 : 06, 6.3 %). The DNA G+C content was 44.2 mol% and the major respiratory quinone was MK-7. On the basis of these polyphasic taxonomic data, MEBiC08714T represents a novel species of the genus Echinicola , for which the name Echinicola strongylocentroti sp. nov. is proposed. The type strain is MEBiC08714T (=KCTC 52052T=JCM 31307T).