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Abstract

A novel halophilic actinomycete strain, designated TRM 46074, was isolated from Aiding salt lake in Turpan Basin, north-west China. Strain TRM 46074 was aerobic, Gram-stain-positive and grew optimally with 12 % (w/v) NaCl. The strain was observed to produce sparse aerial mycelium with long chains of spores that were non-motile and oval or spherical in shape with a rough or smooth surface; substrate mycelium was branched and well developed. The cell wall was determined to contain -diaminopimelic acid; the diagnostic whole-cell sugars were galactose, arabinose and ribose. The diagnostic polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol and phosphatidylinositol. The predominant menaquinones were MK-9(H), MK-10(H) and MK-10(H). The G+C content of the DNA was 70.9 mol%. The major fatty acids were anteiso-C, iso-C, iso-C and iso-C. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain TRM 46074 belongs to the genus . Strain TRM 46074 had 16S rRNA gene sequence similarity of 99.6 % with the closest described species, H53. DNA–DNA hybridization between strain TRM 46074 and H53 showed 58.6 % relatedness. On the basis of the evidence from a polyphasic study, strain TRM 46074 is considered to represent a novel species in the genus , for which the name sp. nov. is proposed. The type strain is TRM 46074 (=CCTCC AA 2012014=JCM 30185).

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2017-03-01
2020-01-22
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