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Abstract

A bacterial strain, designated 5GHs33-3, was isolated from greenhouse soil collected from Yongin region, Gyeonggi province, South Korea. The strain was an aerobic, Gram-stain-positive, flagellated, rod-shaped bacterium. Strain 5GHs33-3 grew at 4–37 °C (optimally at 28–30 °C), pH 6.0–10.0 (optimally at pH 7.0) and with 0–7 % (w/v) NaCl (optimally with 0 %). The 16S rRNA gene sequence analysis revealed that strain 5GHs33-3 had high sequence similarity with EGI 60002 (98.4 %), CF5-4 (96.4 %) and DSM 3133 (96.2 %), and less than 95.5 % sequence similarity against all the other species with validly published names. The phylogenetic tree revealed that strain 5GHs33-3 formed a robust independent monophyletic line with EGI 60002. The predominant fatty acids of strain 5GHs33-3 were anteiso-C and iso-C. The only quinone was MK-8(H). Polar lipids were diphosphatidylglycerol, phosphatidylinositol, an unknown phosphoglycolipid and unknown lipids. The peptidoglycan type was A4, with ornithine as the diagnostic diamino acid and an interpeptide bridge comprising -Glu. The DNA G+C content is 69.0 mol%. Based on phylogenetic evidence and the results of phenotypic, genotypic and chemotaxonomic analyses, strain 5GHs33-3 represents a novel species of a new genus of the family , for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is 5GHs33-3 (=KACC 16542=NBRC 111006). We also propose the reclassification of as comb. nov. (type strain EGI 60002=JCM 19624=KACC 17839=KCTC 29256).

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2017-03-01
2020-01-27
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References

  1. Yi H, Schumann P, Chun J. Demequina aestuarii gen. nov., sp. nov., a novel actinomycete of the suborder Micrococcineae, and reclassification of Cellulomonas fermentans Bagnara et al. 1985 as Actinotalea fermentans gen. nov., comb. nov. Int J Syst Evol Microbiol 2007;57:151–156 [CrossRef][PubMed]
    [Google Scholar]
  2. Li Y, Chen F, Dong K, Wang G. Actinotalea ferrariae sp. nov., isolated from an iron mine, and emended description of the genus Actinotalea. Int J Syst Evol Microbiol 2013;63:3398–3403 [CrossRef][PubMed]
    [Google Scholar]
  3. Zhao S, Li L, Li SH, Wang HF, Hozzein WN et al. Actinotalea suaedae sp. nov., isolated from the halophyte Suaeda physophora in Xinjiang, Northwest China. Antonie van Leeuwenhoek 2015;107:1–7 [CrossRef][PubMed]
    [Google Scholar]
  4. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG et al. (editors) Current Protocols in Molecular Biology New York, NY: Greene/Wiley Interscience; 1987
    [Google Scholar]
  5. Felske A, Rheims H, Wolterink A, Stackebrandt E, Akkermans AD. Ribosome analysis reveals prominent activity of an uncultured member of the class Actinobacteria in grassland soils. Microbiology 1997;143:2983–2989 [CrossRef][PubMed]
    [Google Scholar]
  6. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012;62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  7. Pruesse E, Peplies J, Glöckner FO. SINA: accurate high-throughput multiple sequence alignment of ribosomal RNA genes. Bioinformatics 2012;28:1823–1829 [CrossRef][PubMed]
    [Google Scholar]
  8. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013;30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  9. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406–425[PubMed]
    [Google Scholar]
  10. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981;17:368–376[PubMed][CrossRef]
    [Google Scholar]
  11. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971;20:406–416[CrossRef]
    [Google Scholar]
  12. Tamura K, Nei M. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol 1993;10:512–526[PubMed]
    [Google Scholar]
  13. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: Microbial ID; 1990
    [Google Scholar]
  14. Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984;2:233–241 [CrossRef]
    [Google Scholar]
  15. Hamada M, Yamamura H, Komukai C, Tamura T, Suzuki K et al. Luteimicrobium album sp. nov., a novel actinobacterium isolated from a lichen collected in Japan, and emended description of the genus Luteimicrobium. J Antibiot 2012;65:427–431 [CrossRef][PubMed]
    [Google Scholar]
  16. Gonzalez JM, Saiz-Jimenez C. A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 2002;4:770–773[PubMed][CrossRef]
    [Google Scholar]
  17. Seldin L, Dubnau D. Deoxyribonucleic acid homology among Bacillus polymyxa, Bacillus macerans, Bacillus azotofixans, and other nitrogen-fixing Bacillus strains. Int J Syst Bacteriol 1985;35:151–154 [CrossRef]
    [Google Scholar]
  18. Stackebrandt E, Goebel BM. Taxonomic note: a place for DNA-DNA reassociation and 16s rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 1994;44:846–849 [CrossRef]
    [Google Scholar]
  19. Bagnara C, Toci R, Gaudin C, Belaich JP. Isolation and characterization of a cellulolytic microorganism, Cellulomonas fermentans sp. nov. Int J Syst Bacteriol 1985;35:502–507[CrossRef]
    [Google Scholar]
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