1887

Abstract

A Gram-stain-negative, non-spore-forming, rod-shaped, non-motile bacterium, named wh15, was isolated from the waste water of a municipal wastewater treatment plant in Wuhu, PR China. The taxonomic status of the strain was characterized using a polyphasic taxonomic approach. Growth occurred at 15–45 °C (optimum, 25–40 °C), at pH 5.0–8.0 (optimum, pH 7.0) and with 0–4 % NaCl (optimum, 1–2 %). Phylogenetic analyses based on 16S rRNA gene sequences revealed that wh15 represented a member of genus Flavobacterium and shared the highest similarity with Flavobacterium ummariense DS-12 (93.93 %), Flavobacterium ceti 454–2 (93.71 %) and Flavobacterium marinum SW105 (93.63 %). The major respiratory quinone of wh15 was MK-6 and major fatty acids were iso-C15 : 0, iso-C15 : 0 3-OH, iso-C17 : 0 3-OH, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and summed feature 9 (iso-C17 : 1ω9c and/or C16 : 0 10-methyl). The DNA G+C content of wh15 was 32.3 mol%. Phenotypic, phylogenetic and chemotaxonomic evidence indicated that wh15 represents a novel species of the genus Flavobacterium , the name Flavobacterium cloacae is proposed. The type strain is wh15 (=CCTCC AB 2015420=JCM 31173).

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2017-04-03
2019-12-11
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References

  1. Bergey D, Harrison F, Breed R, Hammer B, Huntoon FM et al. Bergey's Manual of Determinative Bacteriology Baltimore: Williams & Wilkins Co; 1923
    [Google Scholar]
  2. Bernardet JF, Segers P, Vancanneyt M, Berthe F, Kersters K et al. Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Evol Microbiol 1996;46:128–148 [CrossRef]
    [Google Scholar]
  3. Yoon JH, Kang SJ, Oh TK. Flavobacterium soli sp. nov., isolated from soil. Int J Syst Evol Microbiol 2006;56:997–1000 [CrossRef][PubMed]
    [Google Scholar]
  4. Chen WM, Sheu SY, Chen YL. Flavobacterium brevivitae sp. nov., isolated from river water. Int J Syst Evol Microbiol 2016;66:1705–1712 [CrossRef]
    [Google Scholar]
  5. Song L, Liu H, Huang Y, Dai X, Zhou Y. Flavobacterium marinum sp. nov., isolated from seawater. Int J Syst Evol Microbiol 2013;63:3551–3555 [CrossRef][PubMed]
    [Google Scholar]
  6. Fujii D, Nagai F, Watanabe Y, Shirasawa Y. Flavobacterium longum sp. nov. and Flavobacterium urocaniciphilum sp. nov., isolated from a wastewater treatment plant, and emended descriptions of Flavobacterium caeni and Flavobacterium terrigena. Int J Syst Evol Microbiol 2014;64:1488–1494 [CrossRef][PubMed]
    [Google Scholar]
  7. Sun JQ, Xu L, Liu M, Wang XY, Wu XL. Flavobacterium suaedae sp. nov., an endophyte isolated from the root of Suaeda corniculata. Int J Syst Evol Microbiol 2016;66:1943–1949 [CrossRef][PubMed]
    [Google Scholar]
  8. Feng Q, Han L, Nogi Y, Hong Q, Lv J. Flavobacterium lutivivi sp. nov., isolated from activated sludge. Int J Syst Evol Microbiol 2016;65:370–374
    [Google Scholar]
  9. Kim JH, Choi BH, Jo M, Kim SC, Lee PC. Flavobacterium faecale sp. nov., an agarase-producing species isolated from stools of Antarctic penguins. Int J Syst Evol Microbiol 2014;64:2884–2890 [CrossRef][PubMed]
    [Google Scholar]
  10. Nogi Y, Soda K, Oikawa T. Flavobacterium frigidimaris sp. nov., isolated from Antarctic seawater. Syst Appl Microbiol 2005;28:310–315 [CrossRef][PubMed]
    [Google Scholar]
  11. Bernardet JF, Bowman JP. The genus Flavobacterium. In Dworkin M, Falkow S, Rosenberg E, Schleifer KH, Stackebrandt E. et al (editors) The Prokaryotes New York: Springer; 2006; pp.481–531[CrossRef]
    [Google Scholar]
  12. Gerhardt P, Murray R, Wood WA, Krieg NR. Phenotypic characterization. In Gerhardt P. editor Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology.; 1994; pp.607–654
    [Google Scholar]
  13. Dong X, Cai M. Determinative Manual for Routine Bacteriology Beijing: Scientific Press; 2001
    [Google Scholar]
  14. Bernardet JF, Nakagawa Y, Holmes B. Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002;52:1049–1070 [CrossRef][PubMed]
    [Google Scholar]
  15. Gupta SK, Kumari R, Prakash O, Lal R. Pseudomonas panipatensis sp. nov., isolated from an oil-contaminated site. Int J Syst Evol Microbiol 2008;58:1339–1345 [CrossRef][PubMed]
    [Google Scholar]
  16. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012;62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  17. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucl Acids Res 1997;25:4876–4882 [CrossRef][PubMed]
    [Google Scholar]
  18. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013;30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  19. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985;39:783–791 [CrossRef]
    [Google Scholar]
  20. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980;16:111–120 [CrossRef][PubMed]
    [Google Scholar]
  21. Lata P, Lal D, Lal R. Flavobacterium ummariense sp. nov., isolated from hexachlorocyclohexane-contaminated soil, and emended description of Flavobacterium ceti Vela et al. 2007. Int J Syst Evol Microbiol 2012;62:2674–2679 [CrossRef][PubMed]
    [Google Scholar]
  22. Vela AI, Fernandez A, Sánchez-Porro C, Sierra E, Mendez M et al. Flavobacterium ceti sp. nov., isolated from beaked whales (Ziphius cavirostris). Int J Syst Evol Microbiol 2007;57:2604–2608 [CrossRef][PubMed]
    [Google Scholar]
  23. Kacagan M, Inan K, Belduz AO, Canakci S. Flavobacterium anatoliense sp. nov., isolated from fresh water, and emended description of Flavobacterium ceti. Int J Syst Evol Microbiol 2013;63:2075–2081 [CrossRef][PubMed]
    [Google Scholar]
  24. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  25. Kates M. Radioisotopic techniques in lipidology. In Techniques of Lipidology, 2. rev. ed. 1986
    [Google Scholar]
  26. Collins MD, Pirouz T, Goodfellow M, Minnikin DE. Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 1977;100:221–230 [CrossRef][PubMed]
    [Google Scholar]
  27. Tamaoka J, Katayama-Fujimura Y, Kuraishi H. Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. J Appl Microbiol 1983;54:31–36 [CrossRef]
    [Google Scholar]
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