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Abstract

A Gram-stain-positive, aerobic bacterial strain, designated SL15-1, was isolated from desert soil which was sampled from the rhizosphere of , Hangjin Banner, Ordos, Inner Mongolia, northern China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain SL15-1 was clustered with strains, sharing the highest similarity of 16S rRNA gene sequence (96.97 %) with DSM 15626. Strain SL15-1 contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, an unknown phospholipid, an unknown lipid and two unknown aminophospholipids as the major polar lipids. MK-9(H) was the predominant menaquinone, while anteiso-C, iso-C, Cω8 and iso-C were the major cellular fatty acids. Its genomic DNA GC content was 65.3 mol%. The results of physiological and biochemical tests allowed the discrimination of strain SL15-1 from its phylogenetic relatives. sp. nov. is therefore proposed with strain SL15-1 (=CGMCC 1.15906=KCTC 39825) as the type strain.

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2017-03-01
2020-01-24
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References

  1. Park YH, Yoon JH, Shin YK, Suzuki K, Kudo T et al. Classification of 'Nocardioides fulvus' IFO 14399 and Nocardioides sp. ATCC 39419 in Kribbella gen. nov., as Kribbella flavida sp. nov. and Kribbella sandramycini sp. nov. Int J Syst Bacteriol 1999;49:743–752 [CrossRef][PubMed]
    [Google Scholar]
  2. Everest GJ, Curtis SM, de Leo F, Urzì C, Meyers PR. Kribbella albertanoniae sp. nov., isolated from a Roman catacomb, and emended description of the genus Kribbella. Int J Syst Evol Microbiol 2013;63:3591–3596 [CrossRef][PubMed]
    [Google Scholar]
  3. Sohn K, Hong SG, Bae KS, Chun J. Transfer of Hongia koreensis Lee, et al. 2000 to the genus Kribbella Park, et al. 1999 as Kribbella koreensis comb. nov. Int J Syst Evol Microbiol 2003;53:1005–1007 [CrossRef][PubMed]
    [Google Scholar]
  4. Song J, Kim BY, Hong SB, Cho HS, Sohn K et al. Kribbella solani sp. nov. and Kribbella jejuensis sp. nov., isolated from potato tuber and soil in Jeju, Korea. Int J Syst Evol Microbiol 2004;54:1345–1348 [CrossRef][PubMed]
    [Google Scholar]
  5. Cui YS, Lee JS, Lee ST, Im WT. Kribbella ginsengisoli sp. nov., isolated from soil of a ginseng field. Int J Syst Evol Microbiol 2010;60:364–368 [CrossRef][PubMed]
    [Google Scholar]
  6. Li WJ, Wang D, Zhang YQ, Schumann P, Stackebrandt E et al. Kribbella antibiotica sp. nov., a novel nocardioform actinomycete strain isolated from soil in Yunnan, China. Syst Appl Microbiol 2004;27:160–165 [CrossRef][PubMed]
    [Google Scholar]
  7. Li WJ, Wang D, Zhang YQ, Xu LH, Jiang CL. Kribbella yunnanensis sp. nov., Kribbella alba sp. nov., two novel species of genus Kribbella isolated from soils in Yunnan, China. Syst Appl Microbiol 2006;29:29–35 [CrossRef][PubMed]
    [Google Scholar]
  8. Xu Z, Xu Q, Zheng Z, Huang Y. Kribbella amoyensis sp. nov., isolated from rhizosphere soil of a pharmaceutical plant, Typhonium giganteum Engl. Int J Syst Evol Microbiol 2012;62:1081–1085 [CrossRef][PubMed]
    [Google Scholar]
  9. Kirby BM, Le Roes M, Meyers PR. Kribbella karoonensis sp. nov. and Kribbella swartbergensis sp. nov., isolated from soil from the Western Cape, South Africa. Int J Syst Evol Microbiol 2006;56:1097–1101 [CrossRef][PubMed]
    [Google Scholar]
  10. Everest GJ, Meyers PR. Kribbella hippodromi sp. nov., isolated from soil from a racecourse in South Africa. Int J Syst Evol Microbiol 2008;58:443–446 [CrossRef][PubMed]
    [Google Scholar]
  11. Urzì C, de Leo F, Schumann P. Kribbella catacumbae sp. nov. and Kribbella sancticallisti sp. nov., isolated from whitish-grey patinas in the catacombs of St Callistus in Rome, Italy. Int J Syst Evol Microbiol 2008;58:2090–2097 [CrossRef][PubMed]
    [Google Scholar]
  12. Carlsohn MR, Groth I, Spröer C, Schütze B, Saluz HP et al. Kribbella aluminosa sp. nov., isolated from a medieval alum slate mine. Int J Syst Evol Microbiol 2007;57:1943–1947 [CrossRef][PubMed]
    [Google Scholar]
  13. Trujillo ME, Kroppenstedt RM, Schumann P, Martínez-Molina E. Kribbella lupini sp. nov., isolated from the roots of Lupinus angustifolius. Int J Syst Evol Microbiol 2006;56:407–411 [CrossRef][PubMed]
    [Google Scholar]
  14. Wang YN, Cai H, Yu SL, Wang ZY, Liu J et al. Halomonas gudaonensis sp. nov., isolated from a saline soil contaminated by crude oil. Int J Syst Evol Microbiol 2007;57:911–915 [CrossRef][PubMed]
    [Google Scholar]
  15. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013;30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  16. Thompson JD, Gibson TJ, Plewniak F, Jeanpougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997;24:4876–4882[CrossRef]
    [Google Scholar]
  17. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406–425[PubMed]
    [Google Scholar]
  18. Felsenstein J. PHYLIP (Phylogeny Inference Package) Version 3.5.1 Seattle, USA: Department of Genetics, University of Washington; 1993
    [Google Scholar]
  19. Rzhetsky A, Nei M. A simple method for estimating and testing minimum-evolution trees. Mol Biol Evol 1992;9:945–967
    [Google Scholar]
  20. Rzhetsky A, Nei M. Theoretical foundation of the minimum-evolution method of phylogenetic inference. Mol Biol Evol 1993;10:1073–1095[PubMed]
    [Google Scholar]
  21. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985;39:783–791 [CrossRef]
    [Google Scholar]
  22. Yoon S-H, Ha S-M, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA and whole genome assemblies. Int J Syst Evol Microbiol doi:10.1099/ijsem.0.001755 [Epub ahead of print]
    [Google Scholar]
  23. Stackebrandt E, Goebel BM. Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Evol Microbiol 1994;44:846–849 [CrossRef]
    [Google Scholar]
  24. Mandel M, Marmur J. Use of ultraviolet absorbance temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol 1968;12:195–206[CrossRef]
    [Google Scholar]
  25. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101 Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  26. Kates M. Techniques of Lipidology, 2nd ed. Amsterdam: Elsevier; 1986
    [Google Scholar]
  27. Komagata K, Suzuki K. Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 1987;19:161–207[CrossRef]
    [Google Scholar]
  28. Smibert RM, Krieg NR. Phenotypic Characterization. In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994; pp.607–654
    [Google Scholar]
  29. Fraser SL, Jorgensen JH. Reappraisal of the antimicrobial susceptibilities of Chryseobacterium and Flavobacterium species and methods for reliable susceptibility testing. Antimicrob Agents Chemother 1997;41:2738–2741[PubMed]
    [Google Scholar]
  30. Dong XZ, Cai MY. Determinative Manual for Routine Bacteriology Beijing: Scientific Press; 2001
    [Google Scholar]
  31. Kaewkla O, Franco CM. Kribbella endophytica sp. nov., an endophytic actinobacterium isolated from the surface-sterilized leaf of a native apricot tree. Int J Syst Evol Microbiol 2013;63:1249–1253 [CrossRef][PubMed]
    [Google Scholar]
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