- Volume 58, Issue 3, 2008

The purpose of this announcement is to effect the valid publication of the following effectively published new names and new combinations under the procedure described in the Bacteriological Code (1990 Revision). Authors and other individuals wishing to have new names and/or combinations included in future lists should send three copies of the pertinent reprint or photocopies thereof, or an electronic copy of the published paper, to the IJSEM Editorial Office for confirmation that all of the other requirements for valid publication have been met. It is also a requirement of IJSEM and the ICSP that authors of new species, new subspecies and new combinations provide evidence that types are deposited in two recognized culture collections in two different countries (i.e. documents certifying deposition and availability of type strains). It should be noted that the date of valid publication of these new names and combinations is the date of publication of this list, not the date of the original publication of the names and combinations. The authors of the new names and combinations are as given below, and these authors' names will be included in the author index of the present issue and in the volume author index. Inclusion of a name on these lists validates the publication of the name and thereby makes it available in bacteriological nomenclature. The inclusion of a name on this list is not to be construed as taxonomic acceptance of the taxon to which the name is applied. Indeed, some of these names may, in time, be shown to be synonyms, or the organisms may be transferred to another genus, thus necessitating the creation of a new combination.

This listing of names published in a previous issue of the IJSEM is provided as a service to bacteriology to assist in the recognition of new names and new combinations. This procedure was proposed by the Judicial Commission [Minute 11(ii), Int J Syst Bacteriol 41 (1991), p. 185]. The names given herein are listed according to the Rules of priority (i.e. page number and order of valid publication of names in the original articles). Taxonomic opinions included in this List (i.e. the creation of synonyms or the emendation of circumscriptions) cannot be considered as validly published nor, in any other way, approved by the International Committee on Systematics of Prokaryotes and its Judicial Commission.

A Gram-positive, non-spore-forming, rod-shaped, motile bacterium, designated strain DCY22T, was isolated from soil of a ginseng field in South Korea and characterized in order to determine its taxonomic position. 16S rRNA gene sequence analysis revealed that strain DCY22T belonged within the family Sanguibacteraceae, and highest levels of sequence similarity were found with Sanguibacter marinus 1-19T (96.8 %), Sanguibacter suarezii ST-26T (96.0 %), Sanguibacter inulinus ST-50T (95.9 %), Sanguibacter keddieii ST-74T (95.5 %), Terrabacter terrae PPLBT (94.0 %) and Terrabacter tumescens DSM 20308T (93.8 %). Chemotaxonomic investigations revealed that strain DCY22T possessed menaquinone MK-9, a common feature of members of the genus Sanguibacter. Predominant fatty acids were unknown ECL 13.961 (45.81 %), 17 : 0 anteiso (23.46 %), 18 : 0 iso (15.42 %) and unknown ECL 14.966 (8.70 %). The results of physiological and biochemical tests clearly demonstrated that strain DCY22T represents a novel species of the genus Sanguibacter, for which the name Sanguibacter soli sp. nov. is proposed. The type strain is DCY22T (=KCTC 13155T=JCM 14841T).

A Gram-positive, moderately halophilic actinomycete strain, designated YIM 90502T, was isolated from a sample of muddy soil collected from Gulbarga, Karnataka Province, India, and subjected to a polyphasic taxonomical analysis. The isolate grew optimally at 28 °C and in the presence of 10 % (w/v) NaCl. The isolate was characterized chemotaxonomically as having meso-diaminopimelic acid in the cell-wall peptidoglycan and galactose and arabinose as whole-cell sugars. The predominant menaquinone was MK-9(H4), while MK-8(H4) was found in smaller amounts. The phospholipids were phosphatidylinositol, phosphatidylglycerol, diphosphatidylglycerol and phosphatidylethanolamine. The major fatty acids were iso-C16 : 0 (49.2 %) and C17 : 1 ω6c (9.1 %). The DNA G+C content of the isolate was 71.8 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that the isolate fell within the evolutionary radiation encompassed by the genus Saccharomonospora. 16S rRNA gene sequence similarity between strain YIM 90502T and the type strains of Saccharomonospora species ranged from 92.42 % (with Saccharomonospora xinjiangensis CCTCC AA 97021T) to 97.45 % (with Saccharomonospora azurea KCTC 9693T). Levels of DNA–DNA relatedness between strain YIM 90502T and S. azurea KCTC 9693T, Saccharomonospora halophila DSM 44411T and Saccharomonospora paurometabolica DSM 44619T were 46.0, 41.0 and 42.5 %, respectively. On the basis of phenotypic, phylogenetic and genotypic data, strain YIM 90502T was classified in the genus Saccharomonospora as a member of a novel species, for which the name Saccharomonospora saliphila sp. nov. is proposed, with YIM 90502T (=KCTC 19234T =DSM 45087T) as the type strain.

A novel Gram-positive, rod-shaped actinobacterium, designated strain YIM 90718T, was isolated from a saline soil in Xinjiang province, north-west China, and subjected to polyphasic taxonomy. The peptidoglycan type was A1γ and the cell-wall sugars contained galactose. Phospholipids were phosphatidylglycerol and diphosphatidylglycerol. The predominant menaquinone was MK-8(H2). The major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C15 : 0. All of these chemotaxonomic data assigned the new isolate YIM 90718T consistently to the genus Brevibacterium. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YIM 90718T formed a distinct phyletic lineage in the genus Brevibacterium and showed the highest sequence similarity (96.2 %) to Brevibacterium samyangense SST-8T and low similarity (<95.5 %) to other species of the genus Brevibacterium. On the based of the polyphasic evidence, a novel species, Brevibacterium album sp. nov., is proposed, with the type strain YIM 90718T (=DSM 18261T =KCTC 19173T =CCTCC AB 206112T).

A Gram-positive, non-motile, coccoid-shaped bacterium, designated strain DS-8T, was isolated from soil from Dokdo, Korea, and its taxonomic position was investigated by using a polyphasic study. Strain DS-8T grew optimally at 30 °C and pH 6.5–7.5 in the presence of 0.5–1.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain DS-8T joined the type strain of Phycicoccus jejuensis, with a bootstrap resampling value of 92.5 %, and shared 96.5 % 16S rRNA gene sequence similarity with P. jejuensis. The cell-wall peptidoglycan type was based on meso-diaminopimelic acid and the acyl type of the muramic acid was acetyl. The predominant menaquinone was MK-8(H4). The major fatty acids were iso-C15 : 0 and iso-C16 : 0. Major polar lipids detected in strain DS-8T were phosphatidylinositol, phosphatidylglycerol, diphosphatidylglycerol and two unidentified phospholipids. The DNA G+C content was 70.7 mol%. DNA–DNA relatedness data and differential phenotypic properties, together with the phylogenetic distinctiveness, revealed that strain DS-8T differs from P. jejuensis. On the basis of the data obtained, strain DS-8T is considered to represent a novel species of the genus Phycicoccus, for which the name Phycicoccus dokdonensis sp. nov. is proposed. The type strain is DS-8T (=KCTC 19248T=CCUG 54521T).

The taxonomic status of a marine actinomycete isolated from the sponge Haliclona sp. collected from Cebu, Philippines, was established using both phenotypic and genotypic data. Strain SPE 10-1T exhibited chemotaxonomic and morphological characteristics that were consistent with those of members of the genus Saccharopolyspora. It showed a strict requirement for salt and is the first obligate marine bacterium of the genus Saccharopolyspora to be isolated. The principal isoprenoid quinone detected was MK-9(H4). The fatty acid pattern consisted mainly of terminally branched iso and anteiso fatty acids. The DNA G+C content was 72.6 mol%. Analysis of the 16S rRNA gene sequence supported affiliation of the strain with the genus Saccharopolyspora; the type strain of Saccharopolyspora gregorii was the closest phylogenetic relative (96 % sequence similarity). Sequence similarities of strain SPE 10-1T to other type strains of this genus were 93–95 %. It is proposed that strain SPE 10-1T should be classified in the genus Saccharopolyspora as a representative of Saccharopolyspora cebuensis sp. nov. The type strain of Saccharopolyspora cebuensis is SPE 10-1T (=DSM 45019T=CIP 109355T).

A previously undescribed, slowly growing, non-chromogenic mycobacterium, isolated from a Korean patient with a symptomatic pulmonary infection, is described as representing a novel species. Its 16S rRNA gene sequence was unique and phylogenetic analysis based on 16S rRNA gene sequences showed that this organism belonged to the Mycobacterium terrae subclade. Phenotypically, the strain was generally similar to M. terrae and Mycobacterium nonchromogenicum, but its growth rate was slower than those of other M. terrae complex strains. A unique mycolic acid profile and phylogenetic analysis based on two different alternative chronometer molecules, hsp65 and rpoB, confirm the taxonomic status of this strain as a representative of a novel species. The name Mycobacterium senuense sp. nov. is proposed, with the type strain 05-832T (=DSM 44999T =KCTC 19147T).

An aerobic, rod-shaped, Gram-positive, oxidase-negative, catalase-positive bacterial isolate, strain FCC-01T, originating as a contaminant of hairspray was characterized using phenotypic and molecular taxonomic methods. A 16S rRNA gene sequence analysis revealed that the isolate belonged to the genus Microbacterium and represented an evolutionary lineage that was distinct from recognized Microbacterium species. Cell-wall hydrolysate from the isolate contained ornithine and the cell-wall sugars consisted of rhamnose and galactose. The main respiratory quinones were MK-12 (38 %) and MK-11 (35 %). The major cellular fatty acids were anteiso-C15 : 0 (48 %), anteiso-C17 : 0 (35 %) and iso-C16 : 0 (11 %). The DNA G+C content was 69 mol%. The isolate showed <98 % 16S rRNA gene sequence similarity with respect to all of the Microbacterium species with validly published names. On the basis of the morphological, physiological and chemotaxonomic data and the results of the comparative 16S rRNA gene sequence analysis, this isolate represents a novel species of the genus Microbacterium, for which the name Microbacterium hatanonis sp. nov. is proposed. The type strain is FCC-01T (=JCM 14558T =DSM 19179T).

Phylogenetic analysis of ‘Streptomyces tenebrarius’ NBRC 16177 on the basis of the 16S rRNA gene sequence revealed that this organism was related to Streptoalloteichus hindustanus. It possessed glutamic acid, alanine and meso-diaminopimelic acid as cell-wall amino acids, galactose, mannose and glucose as whole-cell sugars and the menaquinones MK-10(H6), MK-10(H4), MK-9(H6) and MK-9(H4). The chemotaxonomic characteristics of this strain were consistent with those of the genus Streptoalloteichus. DNA–DNA hybridization, physiological, biochemical and chemotaxonomic data revealed that ‘Streptomyces tenebrarius’ can be easily differentiated from the other member of the genus Streptoalloteichus and that it merits separate species status. The phenotypic and genetic evidence reveals that ‘Streptomyces tenebrarius’ represents a novel species of the genus Streptoalloteichus; the name Streptoalloteichus tenebrarius (ex Higgins and Kastner) nom. rev., comb. nov. is proposed for this species. The type strain is NBRC 16177T (=ATCC 17920T =DSM 40477T =JCM 4838T =NRRL B-12390T =ISP 5477T).

A previously unknown Gram-positive, obligately aerobic actinomycete, YIM 28A4T, was isolated from a sample of saline soil collected from the Qaidam Basin in Qinghai Province, north-west China, and was investigated using a polyphasic taxonomic approach. The strain grew well on most of the media tested, producing white to pale-yellow substrate mycelium, white aerial mycelium and straight to flexuous hyphae. The substrate mycelium was well developed and fragmented with age; the aerial mycelium produced long, straight spore chains. The spore chains were composed of non-motile, smooth-surfaced, rod-shaped spores. No diffusible pigments were produced on any of the media tested. The strain grew in the presence of 0–10 % (w/v) NaCl and at pH 6.0–8.0, with optimum growth occurring at 3 % (w/v) NaCl and pH 7.0. It grew at 10–37 °C, the optimum growth temperature being 28 °C. Whole-cell hydrolysates of strain YIM 28A4T contained meso-diaminopimelic acid and no diagnostic sugars. The predominant phospholipids were phosphatidylcholine, phosphatidylglycerol and diphosphatidylglycerol. The predominant menaquinones were MK-10, MK-10(H2), MK-11 and MK-11(H2). The major cellular fatty acids were iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0. The DNA G+C content was 67.1 mol%. The morphological and chemotaxonomic characteristics of the isolate matched those described for Nocardiopsis species. A phylogenetic analysis based on 16S rRNA gene sequence comparisons confirmed that strain YIM 28A4T was a member of the genus Nocardiopsis and most closely related to the type strains Nocardiopsis aegyptia DSM 44442T and Nocardiopsis halotolerans DSM 44410T, showing 98.1 and 97.8 % 16S rRNA gene sequence similarity, respectively. Strain YIM 28A4T can be differentiated from these type strains by using phenotypic, phylogenetic and DNA–DNA hybridization data. On the basis of the polyphasic evidence, strain YIM 28A4T represents a novel species of the genus Nocardiopsis, for which the name Nocardiopsis quinghaiensis sp. nov. is proposed. The type strain is YIM 28A4T (=DSM 44739T =CGMCC 4.3494T).

Strains 10-46T and 10-106 were isolated from a water reservoir in Korea. They were Gram-negative, non-motile, non-spore-forming rods, produced yellow-pigmented colonies on nutrient agar, contained MK-6 as the predominant menaquinone and 15 : 0 iso and 17 : 0 iso 3-OH as the major fatty acids and had polar lipid profiles consisting of phosphatidylethanolamine and several unknown aminolipids. Phylogenetic analysis, based on 16S rRNA gene sequencing, showed that the strains were closely related to Chryseobacterium indoltheticum ATCC 27950T, Chryseobacterium scophthalmum LMG 13028T and Chryseobacterium soldanellicola KCTC 12382T (96.9, 96.8 and 96.7 % sequence similarity, respectively) and formed a separate lineage within the genus Chryseobacterium. The combined genotypic and phenotypic data supported the conclusion that the strains represent a novel species of the genus Chryseobacterium, for which the name Chryseobacterium aquaticum sp. nov. is proposed. The type strain is 10-46T (=KCTC 12483T =CECT 7302T).

A rod-shaped, translucent yellow-pigmented, Gram-negative bacterium, strain B390T, was isolated from the bryozoan Schizobrachiella sanguinea collected in the Adriatic Sea, near Rovinj, Croatia. 16S rRNA gene sequence analysis indicated affiliation to the genus Tenacibaculum, with sequence similarity levels of 94.8–97.3 % to type strains of species with validly published names. It grew at 5–34 °C, with optimal growth at 18–26 °C, and only in the presence of NaCl or sea salts. In contrast to other type strains of the genus, strain B390T was able to hydrolyse aesculin. The predominant menaquinone was MK-6 and major fatty acids were iso-C15 : 0, iso-C15 : 0 3-OH and iso-C15 : 1. The DNA G+C content was 31.6 mol%. DNA–DNA hybridization and comparative physiological tests were performed with type strains Tenacibaculum aestuarii JCM 13491T and Tenacibaculum lutimaris DSM 16505T, since they exhibit 16S rRNA gene sequence similarities above 97 %. These data, as well as phylogenetic analyses, suggest that strain B390T (=DSM 18961T =JCM 14633T) should be classified as the type strain of a novel species within the genus Tenacibaculum, for which the name Tenacibaculum adriaticum sp. nov. is proposed.

A yellow-pigmented, Gram-negative, strictly aerobic, rod-shaped bacterium (TDMA-5T) was isolated from a freshwater sample collected at Misasa (Tottori, Japan). The DNA G+C content was 38.6 mol%. Major fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH and summed feature 4 (iso-C15 : 0 2-OH and/or C16 : 1 ω7c). MK-7 was the predominant respiratory quinone. Zeaxanthin was the major carotenoid pigment produced; flexirubin-type pigments were not produced. TDMA-5T was sensitive to gamma-irradiation. The strain degraded gelatin, casein, starch, Tween 80 and DNA. Phylogenetic analysis based on the 16S rRNA gene sequence placed TDMA-5T in a distinct lineage in the family Sphingobacteriaceae, sharing 89.4–93.4 % sequence similarity with members of the nearest genus Pedobacter. Strain TDMA-5T could be distinguished from the other members of the family Sphingobacteriaceae by a number of chemotaxonomic and phenotypic characteristics. Based on its unique phenotypic, genotypic and phylogenetic features, strain TDMA-5T represents a novel genus and species, for which the name Nubsella zeaxanthinifaciens gen. nov., sp. nov. is proposed. The type strain of Nubsella zeaxanthinifaciens is TDMA-5T (=NBRC 102579T =CCUG 54348T).

A non-motile, yellow-pigmented bacterium, designated strain CW9T, was isolated from a water-cooling system in Gwangyang, Republic of Korea. The cells were Gram-negative, catalase- and oxidase-positive, short rods. The major fatty acids were iso-C15 : 0 (45.5 %), iso-C17 : 1 ω9c (14.4 %), iso-C17 : 0 3-OH (13.0 %) and summed feature 3 (comprising iso-C15 : 0 2-OH and/or C16 : 1 ω7c; 8.8 %). The DNA G+C content was 35.6 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain CW9T formed a lineage within the genus Chryseobacterium and was closely related to Chryseobacterium gleum ATCC 35910T (98.4 % sequence similarity) and Chryseobacterium indologenes ATCC 29897T (97.8 % sequence similarity). Phenotypic characteristics and DNA–DNA relatedness data served to distinguish strain CW9T from these two species. On the basis of the evidence presented in this study, strain CW9T represents a novel species of the genus Chryseobacterium, for which the name Chryseobacterium aquifrigidense sp. nov. is proposed. The type strain is CW9T (=KCTC 12894T=JCM 14756T).

A novel thermophilic, sulfur-reducing chemolithoautotroph, strain ABI70S6T, was isolated from a deep-sea hydrothermal field at the Yonaguni Knoll IV, Southern Okinawa Trough. Cells of strain ABI70S6T were motile rods, 0.9–2.0 μm in length and 0.4–0.8 μm in width. Strain ABI70S6T was an obligately anaerobic chemolithotroph, exhibiting hydrogen oxidation coupled with sulfur reduction. Growth was observed at 55–78 °C (optimum, 70 °C), pH 5.0–7.5 (optimum, pH 5.5–6.0) and 0.5–4.5 % NaCl (optimum, 3.0 % NaCl). H2 and elemental sulfur were utilized as electron donor and acceptor, respectively. The major fatty acids were C16 : 0 (40.0 %) and C20 : 1 (60.0 %). The G+C content of genomic DNA was 44.2 mol%. The physiological attributes of strain ABI70S6T are similar to those of species of genera within the family Desulfurobacteriaceae, most of which are thermophilic and chemolithoautotrophic sulfur reducers. However, 16S rRNA gene sequence similarities between the novel isolate and type strains of all species within the family Desulfurobacteriaceae were <87 %, which is close to the similarities found between the novel isolate and members of the family Thermodesulfobacteriaceae (<85 %). Based on physiological and phylogenetic features of the novel isolate, it is proposed that it represents a novel species in a novel genus, Thermosulfidibacter takaii gen. nov., sp. nov., within the phylum Aquificae. The type strain of T. takaii is ABI70S6T (=JCM 13301T=DSM 17441T).

A novel extremely thermophilic sulfur-oxidizing bacterium, strain LS12-2T, was isolated from a deep-sea hydrothermal field at the Yonaguni Knoll IV, Southern Okinawa Trough. Cells of strain LS12-2T were motile rods, 1.5–4.0 μm in length and 0.4–0.5 μm in width. Strain LS12-2T was an obligate chemolithoautotroph that could utilize elemental sulfur or thiosulfate as an electron donor and nitrate or oxygen as an electron acceptor. Growth was observed at 65–85 °C (optimum 70–75 °C), pH 5.8–8.3 (optimum pH 6.9–7.5), 1.0–4.0 % (w/v) NaCl (optimum 2.5 %) and 1.0–7.0 % O2 in the gas phase (optimum 3.0 %). Fatty acids detected were C16 : 0 (8.0 %), C18 : 0 (9.0 %), C18 : 1 (62.5 %) and C20 : 1 (20.5 %). The genomic DNA G+C content was 51.3 mol%. 16S rRNA gene sequence analysis indicated that strain LS12-2T belonged to the genus Hydrogenivirga. Based on physiological and phylogenetic characteristics of the isolate, it is proposed that this strain represents a novel species in the genus Hydrogenivirga, Hydrogenivirga okinawensis sp. nov. The type strain of Hydrogenivirga okinawensis is LS12-2T (=JCM 13302T=DSM 17378T).

A novel marine bacterium, designated strain MKT133T, was isolated from the foot epidermis of a nudibranch, Glossodoris cincta (Mollusca), collected in seawater off the coast of Japan at a depth of 4 m. This bacterium was Gram-negative, motile, mesophilic and strictly aerobic, with small rod-shaped cells. Colonies of the strain after 4–5 days incubation on marine agar 2216 at 30 °C were less than 1 mm in diameter. The strain required salt for growth and contained Q-10 as the predominant respiratory quinone, C18 : 1 ω7c, C16 : 0 and C17 : 1 as major cellular fatty acids and C14 : 0 3-OH as a hydroxy fatty acid. 16S rRNA gene sequence analysis showed that the isolate had highest similarity to Sneathiella chinensis, with 97.2 % sequence similarity to the type strain. Our phylogenetic analysis also revealed that this clade represents a distinct lineage and forms a deep branch with less than 90 % 16S rRNA gene sequence similarity to the members of the eight known orders within the Alphaproteobacteria. Sufficient differences exist to distinguish this strain from Sneathiella chinensis. The name Sneathiella glossodoripedis sp. nov. is proposed, with the type strain MKT133T (=IAM 15419T =KCTC 12842T). The novel order Sneathiellales ord. nov. and family Sneathiellaceae fam. nov. are proposed for the distinct phyletic line represented by the genus Sneathiella.

A Gram-negative, motile and rod-shaped bacterium, designated HU2-65WT, was isolated from freshwater sediment. The strain possessed ubiquinone 8 as the predominant isoprenoid quinone and contained major amounts of ω7-cis-octadecenoic acid and hexadecanoic acid in its cell envelope, which are properties shared by members of the genus Burkholderia. On the basis of 16S rRNA gene sequence similarity, strain HU2-65WT was most closely related to the type strain of Burkholderia xenovorans (98.4 %). The DNA G+C content of strain HU2-65WT was 61.2 mol%, and DNA–DNA relatedness values to type strains of closely related species were found to be much lower than 70 %, indicating that the strain represents a separate genomic species within the genus Burkholderia. Strain HU2-65WT was also differentiated from other species of the genus by physiological and biochemical characteristics. Consequently, strain HU2-65WT is considered to represent a single, novel species of the genus Burkholderia, for which the name Burkholderia sediminicola sp. nov. is proposed, with the type strain HU2-65WT (=KCTC 22086T =LMG 24238T).

A Gram-negative bacterium, designated strain GR15-1T, was isolated from a field cultivated with Korean ginseng. Cells were strictly aerobic, motile with multipolar flagella and rod-shaped. The strain grew optimally at 25–28 °C and pH 6.0–7.0. The predominant fatty acids of strain GR15-1T were C18 : 1 ω7c, C16 : 0 and summed feature 2 (C14 : 0 3-OH and/or iso-C16 : 1 I). The major isoprenoid quinone was ubiquinone 8. The G+C content of the genomic DNA was 63.9 mol%. Phylogenetic analysis showed that strain GR15-1T formed a phyletic cluster with Nevskia ramosa Soe1T, with a 16S rRNA gene sequence similarity of 96.8 %. Based on phenotypic, chemotaxonomic and phylogenetic features, strain GR15-1T represents a novel species within the genus Nevskia, for which the name Nevskia soli sp. nov. is proposed. The type strain is GR15-1T (=KACC 11703T =DSM 19509T).