1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 58, Issue 3

sp. nov., isolated from freshwater sediment Free

Abstract

A Gram-negative, motile and rod-shaped bacterium, designated HU2-65W, was isolated from freshwater sediment. The strain possessed ubiquinone 8 as the predominant isoprenoid quinone and contained major amounts of 7--octadecenoic acid and hexadecanoic acid in its cell envelope, which are properties shared by members of the genus . On the basis of 16S rRNA gene sequence similarity, strain HU2-65W was most closely related to the type strain of (98.4 %). The DNA G+C content of strain HU2-65W was 61.2 mol%, and DNA–DNA relatedness values to type strains of closely related species were found to be much lower than 70 %, indicating that the strain represents a separate genomic species within the genus . Strain HU2-65W was also differentiated from other species of the genus by physiological and biochemical characteristics. Consequently, strain HU2-65W is considered to represent a single, novel species of the genus , for which the name sp. nov. is proposed, with the type strain HU2-65W (=KCTC 22086 =LMG 24238).

  • Published Online:
Keyword(s): PHA, polyhydroxyalkanoate
© SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65502-0
2008-03-01
2023-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/3/565.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65502-0&mimeType=html&fmt=ahah

References

  1. Andrews, J. M.(2001). Determination of minimum inhibitory concentrations. J Antimicrob Chemother 48, 5–16.[CrossRef] [Google Scholar]
  2. Buck, J. D.(1982). Nonstaining (KOH) method for determination of Gram reactions of marine bacteria. Appl Environ Microbiol 44, 992–993. [Google Scholar]
  3. Button, D. K., Schut, F., Quang, P., Martin, R. & Robertson, B. R.(1993). Viability and isolation of marine bacteria by dilution culture: theory, procedures, and initial results. Appl Environ Microbiol 59, 881–891. [Google Scholar]
  4. Coenye, T. & Vandamme, P.(2003). Diversity and significance of Burkholderia species occupying diverse ecological niches. Environ Microbiol 5, 719–729.[CrossRef] [Google Scholar]
  5. Coenye, T., Laevens, S., Willems, A., Ohlén, M., Hannant, W., Govan, J. R., Gillis, M., Falsen, E. & Vandamme, P.(2001).Burkholderia fungorum sp. nov. and Burkholderia caledonica sp. nov., two new species isolated from the environment, animals and human clinical samples. Int J Syst Evol Microbiol 51, 1099–1107.[CrossRef] [Google Scholar]
  6. Coenye, T., Henry, D., Speert, D. P. & Vandamme, P.(2004).Burkholderia phenoliruptrix sp. nov., to accommodate the 2,4,5-trichlorophenoxyacetic acid and halophenol-degrading strain AC1100. Syst Appl Microbiol 27, 623–627.[CrossRef] [Google Scholar]
  7. Cowan, S. T. & Steel, K. J.(1965).Manual for the Identification of Medical Bacteria. London: Cambridge University Press.
  8. Ezaki, T., Hashimoto, Y. & Yabuuchi, E.(1989). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef] [Google Scholar]
  9. Fain, M. G. & Haddock, J. D.(2001). Phenotypic and phylogenetic characterization of Burkholderia (Pseudomonas) sp. strain LB400. Curr Microbiol 42, 269–275. [Google Scholar]
  10. Gillis, M., Van Van, T., Bardin, R., Goor, M., Hebbar, P., Willems, A., Segers, P., Kersters, K., Heulin, T. & other authors(1995). Polyphasic taxonomy in the genus Burkholderia leading to an emended description of the genus and proposition of Burkholderia vietnamiensis sp. nov. for N2-fixing isolates from rice in Vietnam. Int J Syst Bacteriol 45, 274–289.[CrossRef] [Google Scholar]
  11. Goris, J., Dejonghe, W., Falsen, E., De Clerck, E., Geeraerts, B., Willems, A., Top, E. M., Vandamme, P. & De Vos, P.(2002). Diversity of transconjugants that acquired plasmid pJP4 or pEMT1 after inoculation of a donor strain in the A- and B-horizon of an agricultural soil and description of Burkholderia hospita sp. nov. and Burkholderia terricola sp. nov. Syst Appl Microbiol 25, 340–352.[CrossRef] [Google Scholar]
  12. Goris, J., De Vos, P., Caballero-Mellado, J., Park, J., Falsen, E., Quensen, J. F., III, Tiedje, J. M. & Vandamme, P.(2004). Classification of the biphenyl- and polychlorinated biphenyl-degrading strain LB400T and relatives as Burkholderia xenovorans sp. nov. Int J Syst Evol Microbiol 54, 1677–1681.[CrossRef] [Google Scholar]
  13. Hall, T. A.(1999). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41, 95–98. [Google Scholar]
  14. Hugh, R. & Leifson, E.(1953). The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various Gram negative bacteria. J Bacteriol 66, 24–26. [Google Scholar]
  15. Kim, H.-B., Park, M.-J., Yang, H.-C., An, D.-S., Jin, H.-Z. & Yang, D.-C.(2006).Burkholderia ginsengisoli sp. nov., a β-glucosidase-producing bacterium isolated from soil of a ginseng field. Int J Syst Evol Microbiol 56, 2529–2533.[CrossRef] [Google Scholar]
  16. Komagata, K. & Suzuki, K.(1987). Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 19, 161–207. [Google Scholar]
  17. Lane, D. J.(1991). 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by E. Stackebrandt & M. Goodfellow. Chichester: Wiley.
  18. Lane, D. J., Pace, B., Olsen, G. J., Stahl, D. A., Sogin, M. L. & Pace, N. R.(1985). Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses. Proc Natl Acad Sci U S A 82, 6955–6959.[CrossRef] [Google Scholar]
  19. Lim, Y. W., Baik, K. S., Han, S. K., Kim, S. B. & Bae, K. S.(2003).Burkholderia sordidicola sp. nov., isolated from the white-rot fungus Phanerochaete sordida. Int J Syst Evol Microbiol 53, 1631–1636.[CrossRef] [Google Scholar]
  20. Martens-Habbena, W. & Sass, H.(2006). Sensitive determination of microbial growth by nucleic acid staining in aqueous suspension. Appl Environ Microbiol 72, 87–95.[CrossRef] [Google Scholar]
  21. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  22. Muyzer, G., de Waal, E. C. & Uitterlinden, A. G.(1993). Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl Environ Microbiol 59, 695–700. [Google Scholar]
  23. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  24. Solaiman, D. K., Ashby, R. D. & Foglia, T. A.(2000). Rapid and specific identification of medium-chain-length polyhydroxyalkanoate synthase gene by polymerase chain reaction. Appl Microbiol Biotechnol 53, 690–694.[CrossRef] [Google Scholar]
  25. Spiekermann, P., Rehm, B. H. A., Kalscheuer, R., Baumeister, D. & Steinbüchel, A.(1999). A sensitive, viable-colony staining method using Nile red for direct screening of bacteria that accumulate polyhydroxyalkanoic acids and other lipid storage compounds. Arch Microbiol 171, 73–80.[CrossRef] [Google Scholar]
  26. Stackebrandt, E. & Liesack, W.(1993). Nucleic acids and classification. In Handbook of New Bacterial Systematics, pp. 152–189. Edited by M. Goodfellow & A. G. O'Donnell. London: Academic Press.
  27. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  28. Vandamme, P., Opelt, K., Knöchel, N., Berg, C., Schönmann, S., De Brandt, E., Eberl, L., Falsen, E. & Berg, G.(2007).Burkholderia bryophila sp. nov. and Burkholderia megapolitana sp. nov., moss-associated species with antifungal and plant-growth-promoting properties. Int J Syst Evol Microbiol 57, 2228–2235.[CrossRef] [Google Scholar]
  29. Weisburg, W. G., Barns, S. M., Pelletier, D. A. & Lane, D. J.(1991). 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173, 697–703. [Google Scholar]
  30. Wieczorek, R., Steinbüchel, A. & Schmidt, B.(1996). Occurrence of polyhydroxyalkanoic acid granule-associated proteins related to the Alcaligenes eutrophus H16 GA24 protein in other bacteria. FEMS Microbiol Lett 135, 23–30.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65502-0
Loading
Burkholderia sediminicola sp. nov., isolated from freshwater sediment
Int J Syst Evol Microbiol 58, 565 (2008); https://doi.org/10.1099/ijs.0.65502-0
/content/journal/ijsem/10.1099/ijs.0.65502-0
/content/journal/ijsem/10.1099/ijs.0.65502-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error