1887

Abstract

A Gram-positive, moderately halophilic actinomycete strain, designated YIM 90502, was isolated from a sample of muddy soil collected from Gulbarga, Karnataka Province, India, and subjected to a polyphasic taxonomical analysis. The isolate grew optimally at 28 °C and in the presence of 10 % (w/v) NaCl. The isolate was characterized chemotaxonomically as having -diaminopimelic acid in the cell-wall peptidoglycan and galactose and arabinose as whole-cell sugars. The predominant menaquinone was MK-9(H), while MK-8(H) was found in smaller amounts. The phospholipids were phosphatidylinositol, phosphatidylglycerol, diphosphatidylglycerol and phosphatidylethanolamine. The major fatty acids were iso-C (49.2 %) and C 6 (9.1 %). The DNA G+C content of the isolate was 71.8 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that the isolate fell within the evolutionary radiation encompassed by the genus . 16S rRNA gene sequence similarity between strain YIM 90502 and the type strains of species ranged from 92.42 % (with CCTCC AA 97021) to 97.45 % (with KCTC 9693). Levels of DNA–DNA relatedness between strain YIM 90502 and KCTC 9693, DSM 44411 and DSM 44619 were 46.0, 41.0 and 42.5 %, respectively. On the basis of phenotypic, phylogenetic and genotypic data, strain YIM 90502 was classified in the genus as a member of a novel species, for which the name sp. nov. is proposed, with YIM 90502 (=KCTC 19234 =DSM 45087) as the type strain.

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2008-03-01
2024-12-06
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Supplements

vol. , part 3, pp. 570 - 573

Scanning electron micrograph of spores of sp. nov. YIM 90502 .

Neighbour-joining tree showing the phylogenetic relationships of strain sp. nov. YIM 90502 within the radiation of the family .

[PDF file of Supplementary Figs S1 and S2](130 KB)



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