A novel thermophilic, sulfur-reducing chemolithoautotroph, strain ABI70S6, was isolated from a deep-sea hydrothermal field at the Yonaguni Knoll IV, Southern Okinawa Trough. Cells of strain ABI70S6 were motile rods, 0.9–2.0 μm in length and 0.4–0.8 μm in width. Strain ABI70S6 was an obligately anaerobic chemolithotroph, exhibiting hydrogen oxidation coupled with sulfur reduction. Growth was observed at 55–78 °C (optimum, 70 °C), pH 5.0–7.5 (optimum, pH 5.5–6.0) and 0.5–4.5 % NaCl (optimum, 3.0 % NaCl). H and elemental sulfur were utilized as electron donor and acceptor, respectively. The major fatty acids were C (40.0 %) and C (60.0 %). The G+C content of genomic DNA was 44.2 mol%. The physiological attributes of strain ABI70S6 are similar to those of species of genera within the family , most of which are thermophilic and chemolithoautotrophic sulfur reducers. However, 16S rRNA gene sequence similarities between the novel isolate and type strains of all species within the family were <87 %, which is close to the similarities found between the novel isolate and members of the family (<85 %). Based on physiological and phylogenetic features of the novel isolate, it is proposed that it represents a novel species in a novel genus, gen. nov., sp. nov., within the phylum . The type strain of is ABI70S6 (=JCM 13301=DSM 17441).


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vol. , part 3, pp. 659 – 665

Effects of temperature, pH and NaCl concentration on the growth of strain ABI70S6

Phylogenetic tree of representative deeply branching members of the including environmental phylotypes based on 16S rRNA gene sequences

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