A previously undescribed, slowly growing, non-chromogenic mycobacterium, isolated from a Korean patient with a symptomatic pulmonary infection, is described as representing a novel species. Its 16S rRNA gene sequence was unique and phylogenetic analysis based on 16S rRNA gene sequences showed that this organism belonged to the subclade. Phenotypically, the strain was generally similar to and , but its growth rate was slower than those of other complex strains. A unique mycolic acid profile and phylogenetic analysis based on two different alternative chronometer molecules, and , confirm the taxonomic status of this strain as a representative of a novel species. The name sp. nov. is proposed, with the type strain 05-832 (=DSM 44999 =KCTC 19147).


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  1. Butler, W. R., Thibert, L. & Kilburn, J. O.(1992). Identification of Mycobacterium avium complex strains and some similar species by high-performance liquid chromatography. J Clin Microbiol 30, 2698–2704. [Google Scholar]
  2. Cloud, J. L., Meyer, J. J., Pounder, J. I., Jost, K. C., Jr, Sweeney, A., Carroll, K. C. & Woods, G. L.(2006).Mycobacterium arupense sp. nov., a non-chromogenic bacterium isolated from clinical specimens. Int J Syst Evol Microbiol 56, 1413–1418.[CrossRef] [Google Scholar]
  3. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  4. Fitch, W. M.(1971). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef] [Google Scholar]
  5. Jukes, T. H. & Cantor, C. R.(1969). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  6. Kent, P. T. & Kubica, G. P.(1985).Public Health Mycobacteriology. Guide for the Level III Laboratory. US Department of Health and Human Services publication. Atlanta: Centers for Disease Control and Prevention.
  7. Kim, B.-J., Lee, S.-H., Lyu, M.-A., Kim, S.-J., Bai, G.-H., Kim, S.-J., Chae, G.-T., Kim, E.-C. & other authors(1999). Identification of mycobacterial species by comparative sequence analysis of the RNA polymerase gene (rpoB). J Clin Microbiol 37, 1714–1720. [Google Scholar]
  8. Kim, B.-J., Lee, K.-H., Park, B.-N., Kim, S.-J., Bai, G.-H., Kim, S.-J. & Kook, Y.-H.(2001). Differentiation of mycobacterial species by PCR-restriction analysis of DNA (342 base pairs) of the RNA polymerase gene (rpoB). J Clin Microbiol 39, 2102–2109.[CrossRef] [Google Scholar]
  9. Kim, H., Kim, S.-H., Shim, T.-S., Kim, M.-n., Bai, G.-H., Park, Y.-G., Lee, S.-H., Chae, G.-T., Cha, C.-Y. & other authors(2005). Differentiation of Mycobacterium species by analysis of the heat-shock protein 65 gene (hsp65). Int J Syst Evol Microbiol 55, 1649–1656.[CrossRef] [Google Scholar]
  10. Kim, H.-J., Mun, H.-S., Kim, H., Oh, E.-J., Ha, Y., Bai, G.-H., Park, Y.-G., Cha, C.-Y., Kook, Y.-H. & Kim, B.-J.(2006). Differentiation of mycobacterial species by hsp65 duplex PCR followed by duplex-PCR-based restriction analysis and direct sequencing. J Clin Microbiol 44, 3855–3862.[CrossRef] [Google Scholar]
  11. Kumar, S., Tamura, K., Jakobsen, I.-B. & Nei, M.(2001).mega2: molecular evolutionary genetics analysis software. Tempe, AZ: Arizona State University.
  12. Minnikin, D. E., Minnikin, S. M., Parlett, J. H., Goodfellow, M. & Magnusson, M.(1984). Mycolic acid patterns of some species of Mycobacterium. Arch Microbiol 139, 225–231. [Google Scholar]
  13. Mun, H.-S., Kim, H.-J., Oh, E.-J., Kim, H., Bai, G.-H., Yu, H.-K., Park, Y.-G., Cha, C.-Y., Kook, Y.-H. & Kim, B.-J.(2007).Mycobacterium seoulense sp. nov., a slowly growing scotochromogenic species. Int J Syst Evol Microbiol 57, 594–599.[CrossRef] [Google Scholar]
  14. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  15. Springer, B., Stockman, L., Teschner, K., Roberts, G. D. & Böttger, E. C.(1996). Two-laboratory collaborative study on identification of mycobacteria: molecular versus phenotypic methods. J Clin Microbiol 34, 296–303. [Google Scholar]
  16. Stahl, D. A. & Urbance, J. W.(1990). The division between fast- and slow-growing species corresponds to natural relationships among the mycobacteria. J Bacteriol 172, 116–124. [Google Scholar]
  17. Stone, B. B., Nietupski, R. M., Breton, G. L. & Weisburg, W. G.(1995). Comparison of Mycobacterium 23S rRNA sequences by high-temperature reverse transcription and PCR. Int J Syst Bacteriol 45, 811–819.[CrossRef] [Google Scholar]
  18. Turenne, C. Y., Cook, V. J., Burdz, T. V., Pauls, R. J., Thibert, L., Wolfe, J. N. & Kabani, A.(2004).Mycobacterium parascrofulaceum sp. nov., novel slowly growing, scotochromogenic clinical isolates related to Mycobacterium simiae. Int J Syst Evol Microbiol 54, 1543–1551.[CrossRef] [Google Scholar]

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