1887

Abstract

A previously unknown Gram-positive, obligately aerobic actinomycete, YIM 28A4, was isolated from a sample of saline soil collected from the Qaidam Basin in Qinghai Province, north-west China, and was investigated using a polyphasic taxonomic approach. The strain grew well on most of the media tested, producing white to pale-yellow substrate mycelium, white aerial mycelium and straight to flexuous hyphae. The substrate mycelium was well developed and fragmented with age; the aerial mycelium produced long, straight spore chains. The spore chains were composed of non-motile, smooth-surfaced, rod-shaped spores. No diffusible pigments were produced on any of the media tested. The strain grew in the presence of 0–10 % (w/v) NaCl and at pH 6.0–8.0, with optimum growth occurring at 3 % (w/v) NaCl and pH 7.0. It grew at 10–37 °C, the optimum growth temperature being 28 °C. Whole-cell hydrolysates of strain YIM 28A4 contained -diaminopimelic acid and no diagnostic sugars. The predominant phospholipids were phosphatidylcholine, phosphatidylglycerol and diphosphatidylglycerol. The predominant menaquinones were MK-10, MK-10(H), MK-11 and MK-11(H). The major cellular fatty acids were iso-C, anteiso-C and anteiso-C. The DNA G+C content was 67.1 mol%. The morphological and chemotaxonomic characteristics of the isolate matched those described for s species. A phylogenetic analysis based on 16S rRNA gene sequence comparisons confirmed that strain YIM 28A4 was a member of the genus and most closely related to the type strains DSM 44442 and DSM 44410, showing 98.1 and 97.8 % 16S rRNA gene sequence similarity, respectively. Strain YIM 28A4 can be differentiated from these type strains by using phenotypic, phylogenetic and DNA–DNA hybridization data. On the basis of the polyphasic evidence, strain YIM 28A4 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YIM 28A4 (=DSM 44739 =CGMCC 4.3494).

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2008-03-01
2019-10-14
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