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Abstract

A non-motile, yellow-pigmented bacterium, designated strain CW9, was isolated from a water-cooling system in Gwangyang, Republic of Korea. The cells were Gram-negative, catalase- and oxidase-positive, short rods. The major fatty acids were iso-C (45.5 %), iso-C 9 (14.4 %), iso-C 3-OH (13.0 %) and summed feature 3 (comprising iso-C 2-OH and/or C 7; 8.8 %). The DNA G+C content was 35.6 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain CW9 formed a lineage within the genus and was closely related to ATCC 35910 (98.4 % sequence similarity) and ATCC 29897 (97.8 % sequence similarity). Phenotypic characteristics and DNA–DNA relatedness data served to distinguish strain CW9 from these two species. On the basis of the evidence presented in this study, strain CW9 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CW9 (=KCTC 12894=JCM 14756).

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2008-03-01
2020-01-20
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References

  1. Baik, K. S., Park, Y.-D., Seong, C. N., Kim, E. M., Bae, K. S. & Chun, J. ( 2006; ). Glaciecola nitratireducens sp. nov. isolated from seawater. Int J Syst Evol Microbiol 56, 2185–2188.[CrossRef]
    [Google Scholar]
  2. Barrow, G. I. & Feltham, R. K. A. ( 1993; ). Cowan and Steel's Manual for the Identification of Medical Bacteria, 3rd edn. Cambridge: Cambridge University Press.
  3. Bernardet, J.-F., Nakagawa, Y. & Holmes, B. ( 2002; ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52, 1049–1070.[CrossRef]
    [Google Scholar]
  4. Campbell, L. L. & Williams, O. B. ( 1951; ). A study of chitin-decomposing micro-organisms of marine origin. J Gen Microbiol 5, 894–905.[CrossRef]
    [Google Scholar]
  5. Chun, J. & Goodfellow, M. ( 1995; ). A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 45, 240–245.[CrossRef]
    [Google Scholar]
  6. Chun, J., Bae, K. S., Moon, E. Y., Jung, S. O., Lee, H. K. & Kim, S. J. ( 2000; ). Nocardiopsis kunsanensis sp. nov., a moderately halophilic actinomycete isolated from a saltern. Int J Syst Evol Microbiol 50, 1909–1913.
    [Google Scholar]
  7. de Beer, H., Hugo, C. J., Jooste, P. J., Willems, A., Vancanneyt, M., Coenye, T. & Vandamme, P. ( 2005; ). Chryseobacterium vrystaatense sp. nov., isolated from raw chicken in a chicken processing plant. Int J Syst Evol Microbiol 55, 2149–2153.[CrossRef]
    [Google Scholar]
  8. de Beer, H., Hugo, C. J., Jooste, P. J., Vancanneyt, M., Coenye, T. & Vandamme, P. ( 2006; ). Chryseobacterium piscium sp. nov., isolated from fish of the South Atlantic Ocean off South Africa. Int J Syst Evol Microbiol 56, 1317–1322.[CrossRef]
    [Google Scholar]
  9. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  10. Fitch, W. M. ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  11. Fitch, W. M. & Margoliash, E. ( 1967; ). Construction of phylogenetic trees. Science 155, 279–284.[CrossRef]
    [Google Scholar]
  12. Gallego, V., Teresa, M., Garcia, M. T. & Ventosa, A. ( 2006; ). Chryseobacterium hispanicum sp. nov., isolated from the drinking water distribution system of Sevilla, Spain. Int J Syst Evol Microbiol 56, 1589–1592.[CrossRef]
    [Google Scholar]
  13. Gosink, J. J., Woese, C. R. & Staley, J. T. ( 1998; ). Polaribacter gen. nov., with three new species, P. irgensii sp. nov., P. franzmannii sp. nov. and P. filamentus sp. nov., gas vacuolated polar marine bacteria of the Cytophaga–Flavobacterium–Bacteroides group and reclassification of ‘Flectobacillus glomeratus’ as Polaribacter glomeratus comb. nov. Int J Syst Bacteriol 48, 223–235.[CrossRef]
    [Google Scholar]
  14. Holmes, B., Owen, R. J., Steigerwalt, A. G. & Brenner, D. J. ( 1984; ). Flavobacterium gleum, a new species found in human clinical specimens. Int J Syst Bacteriol 34, 21–25.[CrossRef]
    [Google Scholar]
  15. Hugo, C. J., Segers, P., Hoste, B., Vancanneyt, M. & Kersters, K. ( 2003; ). Chryseobacterium joostei sp. nov., isolated from the dairy environment. Int J Syst Evol Microbiol 53, 771–777.[CrossRef]
    [Google Scholar]
  16. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  17. Kämpfer, P., Dreyer, U., Neef, A., Dott, W. & Busse, H.-J. ( 2003; ). Chryseobacterium defluvii sp. nov., isolated from wastewater. Int J Syst Evol Microbiol 53, 93–97.[CrossRef]
    [Google Scholar]
  18. Kim, K. K., Bae, H. S., Schumann, P. & Lee, S. T. ( 2005; ). Chryseobacterium daecheongense sp. nov., isolated from freshwater lake sediment. Int J Syst Evol Microbiol 55, 133–138.[CrossRef]
    [Google Scholar]
  19. Kovács, N. ( 1956; ). Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 178, 703
    [Google Scholar]
  20. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  21. MIDI ( 1999; ). Sherlock Microbial Identification System Operating Manual, version 3.0. Newark, DE: MIDI, Inc.
  22. Mudarris, M., Austin, B., Segers, P., Vancanneyt, M., Hoste, B. & Bernardet, J.-F. ( 1994; ). Flavobacterium scophthalmum sp. nov., a pathogen of turbot (Scophthalmus maximus L.). Int J Syst Bacteriol 44, 447–453.[CrossRef]
    [Google Scholar]
  23. NCCLS ( 2003; ). Performance Standards for Antimicrobial Disk Susceptibility Tests, 8th edn. Approved Standard, M2–A8. Wayne, PA: National Committee for Clinical Laboratory Standards.
  24. Park, M. S., Jung, S. R., Lee, K. H., Lee, M.-S., Do, J. O., Kim, S. B. & Bae, K. S. ( 2006; ). Chryseobacterium soldanellicola sp. nov. and Chryseobacterium taeanense sp. nov., isolated from roots of sand-dune plants. Int J Syst Evol Microbiol 56, 433–438.[CrossRef]
    [Google Scholar]
  25. Quan, Z.-X., Kim, K. K., Kim, M.-K., Jin, L. & Lee, S.-T. ( 2007; ). Chryseobacterium caeni sp. nov., isolated from bioreactor sludge. Int J Syst Evol Microbiol 57, 141–145.[CrossRef]
    [Google Scholar]
  26. Richard, C. & Kiredjian, M. ( 1995; ). Laboratory Methods for the Identification of Strictly Aerobic Gram-negative Bacilli. Paris: Institut Pasteur.
  27. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  28. Shen, F. T., Kämpfer, P., Young, C. C., Lai, W. A. & Arun, A. B. ( 2005; ). Chryseobacterium taichungense sp. nov., isolated from contaminated soil. Int J Syst Evol Microbiol 55, 1301–1304.[CrossRef]
    [Google Scholar]
  29. Shimomura, K., Kaji, S. & Hiraishi, A. ( 2005; ). Chryseobacterium shigense sp. nov., a yellow-pigmented, aerobic bacterium isolated from a lactic acid beverage. Int J Syst Evol Microbiol 55, 1903–1906.[CrossRef]
    [Google Scholar]
  30. Skerman, V. B. D. ( 1967; ). A Guide to the Identification of the Genera of Bacteria, 2nd edn. Baltimore: Williams & Wilkins.
  31. Swofford, D. L. ( 1998; ). paup: phylogenetic analysis using parsimony, version 4. Sunderland, MA: Sinauer Associates.
  32. Tai, C.-J., Kuo, H.-P., Lee, F.-L., Che, H.-K., Yokota, A. & Lo, C.-C. ( 2006; ). Chryseobacterium taiwanense sp. nov., isolated from soil in Taiwan. Int J Syst Evol Microbiol 56, 1771–1776.[CrossRef]
    [Google Scholar]
  33. Vandamme, P., Bernardet, J.-F., Segers, P., Kersters, K. & Holmes, B. ( 1994; ). New perspectives in the classification of the flavobacteria: description of Chryseobacterium gen. nov., Bergeyella gen. nov., and Empedobacter nom. rev. Int J Syst Bacteriol 44, 827–831.[CrossRef]
    [Google Scholar]
  34. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  35. Weeks, O. B. ( 1981; ). Preliminary studies of the pigments of Flavobacterium breve NCTC 11099 and Flavobacterium odoratum NCTC 11036. In The Flavobacterium–Cytophaga Group, pp. 109–114. Edited by H. Reichenbach & O. B. Weeks. Weinheim: Gesellschaft für Biotechnologische Forschung.
  36. Weon, H. Y., Kim, B.-Y., Yoo, S.-H., Kwon, S.-W., Cho, Y.-H., Go, S. J. & Stackebrandt, E. ( 2006; ). Chryseobacterium wanjuense sp. nov., isolated from greenhouse soil in Korea. Int J Syst Evol Microbiol 56, 1501–1504.[CrossRef]
    [Google Scholar]
  37. Yabuuchi, E., Kaneko, T., Yano, I., Moss, C. W. & Miyoshi, N. ( 1983; ). Sphingobacterium gen. nov., Sphingobacterium spiritivorum comb. nov., Sphingobacterium multivorum comb. nov., Sphingobacterium mizutae sp. nov., and Flavobacterium indologenes sp. nov.: glucose nonfermenting Gram-negative rods in CDC groups IIK-2 and IIb. Int J Syst Bacteriol 33, 580–598.[CrossRef]
    [Google Scholar]
  38. Yamaguchi, S. & Yokoe, M. ( 2000; ). A novel protein-deamidating enzyme from Chryseobacterium proteolyticum sp. nov., a newly isolated bacterium from soil. Appl Environ Microbiol 66, 3337–3343.[CrossRef]
    [Google Scholar]
  39. Yoon, J.-H., Kang, S.-J. & Oh, T.-K. ( 2007; ). Chryseobacterium daeguense sp. nov., isolated from wastewater of a textile dye works. Int J Syst Evol Microbiol 57, 1355–1359.[CrossRef]
    [Google Scholar]
  40. Young, C. C., Kämpfer, P., Shen, F. T., Lai, W. A. & Arun, A. B. ( 2005; ). Chryseobacterium formosense sp. nov., isolated from the rhizosphere of Lactuca sativa L. (garden lettuce). Int J Syst Evol Microbiol 55, 423–426.[CrossRef]
    [Google Scholar]
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vol. , part 3, pp. 607 - 611

Results of the GN2 MicroPlate (Biolog) test for strain CW9 after 24 h incubation at 30°C. [ PDF] 38 KB



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