A yellow-pigmented, Gram-negative, strictly aerobic, rod-shaped bacterium (TDMA-5) was isolated from a freshwater sample collected at Misasa (Tottori, Japan). The DNA G+C content was 38.6 mol%. Major fatty acids were iso-C, iso-C 3-OH and summed feature 4 (iso-C 2-OH and/or C 7). MK-7 was the predominant respiratory quinone. Zeaxanthin was the major carotenoid pigment produced; flexirubin-type pigments were not produced. TDMA-5 was sensitive to gamma-irradiation. The strain degraded gelatin, casein, starch, Tween 80 and DNA. Phylogenetic analysis based on the 16S rRNA gene sequence placed TDMA-5 in a distinct lineage in the family , sharing 89.4–93.4 % sequence similarity with members of the nearest genus . Strain TDMA-5 could be distinguished from the other members of the family by a number of chemotaxonomic and phenotypic characteristics. Based on its unique phenotypic, genotypic and phylogenetic features, strain TDMA-5 represents a novel genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain of is TDMA-5 (=NBRC 102579 =CCUG 54348).


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Phylogenetic trees based on the 16S rRNA gene sequences of strain TDMA-5 and representative members of related genera in the family . [PDF](21 KB)


Scanning electron micrograph of strain TDMA-5 grown on LB agar at 30 °C. Cells were long rods after 3 days cultivation. Bar, 1 µm.


Whole-cell fatty acid profile of strain TDMA-5 . [PDF](21 KB)

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