1887

Abstract

A novel Gram-positive, rod-shaped actinobacterium, designated strain YIM 90718, was isolated from a saline soil in Xinjiang province, north-west China, and subjected to polyphasic taxonomy. The peptidoglycan type was A1 and the cell-wall sugars contained galactose. Phospholipids were phosphatidylglycerol and diphosphatidylglycerol. The predominant menaquinone was MK-8(H). The major fatty acids were anteiso-C, anteiso-C and iso-C. All of these chemotaxonomic data assigned the new isolate YIM 90718 consistently to the genus . Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YIM 90718 formed a distinct phyletic lineage in the genus and showed the highest sequence similarity (96.2 %) to SST-8 and low similarity (<95.5 %) to other species of the genus . On the based of the polyphasic evidence, a novel species, sp. nov., is proposed, with the type strain YIM 90718 (=DSM 18261 =KCTC 19173 =CCTCC AB 206112).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65183-0
2008-03-01
2020-01-26
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/3/574.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65183-0&mimeType=html&fmt=ahah

References

  1. Breed, R. S. ( 1953; ). The families developed from Bacteriaceae Cohn with a description of the family Brevibacteriaceae. Rias Commun VI Int Congr Microbiol Roma 1, 10–15.
    [Google Scholar]
  2. Cerny, G. ( 1978; ). Studies on aminopeptidase for the distinction of Gram-negative from Gram-positive bacteria. Eur J Appl Microbiol Biotechnol 5, 113–122.[CrossRef]
    [Google Scholar]
  3. Collins, M. D., Jones, D., Keddie, R. M. & Sneath, P. H. A. ( 1980; ). Reclassification of Chromobacterium iodinum (Davis) in a redefined genus Brevibacterium (Breed) as Brevibacterium iodinum nom. rev.; comb. nov. J Gen Microbiol 120, 1–10.
    [Google Scholar]
  4. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  5. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  6. Fitch, W. M. ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  7. Hasegawa, T., Takizawa, M. & Tanida, S. ( 1983; ). A rapid analysis for chemical grouping of aerobic Actinomycetes. J Gen Appl Microbiol 29, 319–322.[CrossRef]
    [Google Scholar]
  8. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  9. Kroppenstedt, R. M. ( 1982; ). Separation of bacterial menaquinones by HPLC using reverse phase (RP 18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr 5, 2359–2367.[CrossRef]
    [Google Scholar]
  10. Kumar, S., Tamura, K., Jakobsen, I.-B. & Nei, M. ( 2001; ). mega2: molecular evolutionary genetics analysis software. Bioinformatics 17, 1244–1245.[CrossRef]
    [Google Scholar]
  11. Lee, S. D. ( 2006; ). Brevibacterium samyangense sp. nov., an actinomycete isolated from a beach sediment. Int J Syst Evol Microbiol 56, 1889–1892.[CrossRef]
    [Google Scholar]
  12. Leifson, E. ( 1960; ). Atlas of Bacterial Flagellation. London: Academic Press.
  13. Li, W.-J., Xu, P., Schumann, P., Zhang, Y.-Q., Pukall, R., Xu, L.-H., Stackebrandt, E. & Jiang, C.-L. ( 2007; ). Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia. Int J Syst Evol Microbiol 57, 1424–1428.[CrossRef]
    [Google Scholar]
  14. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  15. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  16. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H. ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef]
    [Google Scholar]
  17. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  18. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20, 16
    [Google Scholar]
  19. Schleifer, K. H. & Kandler, O. ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477.
    [Google Scholar]
  20. Shirling, E. B. & Gottlieb, D. ( 1966; ). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef]
    [Google Scholar]
  21. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65183-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65183-0
Loading

Data & Media loading...

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error