1887

Abstract

A novel marine bacterium, designated strain MKT133, was isolated from the foot epidermis of a nudibranch, (Mollusca), collected in seawater off the coast of Japan at a depth of 4 m. This bacterium was Gram-negative, motile, mesophilic and strictly aerobic, with small rod-shaped cells. Colonies of the strain after 4–5 days incubation on marine agar 2216 at 30 °C were less than 1 mm in diameter. The strain required salt for growth and contained Q-10 as the predominant respiratory quinone, C 7, C and C as major cellular fatty acids and C 3-OH as a hydroxy fatty acid. 16S rRNA gene sequence analysis showed that the isolate had highest similarity to , with 97.2 % sequence similarity to the type strain. Our phylogenetic analysis also revealed that this clade represents a distinct lineage and forms a deep branch with less than 90 % 16S rRNA gene sequence similarity to the members of the eight known orders within the . Sufficient differences exist to distinguish this strain from . The name sp. nov. is proposed, with the type strain MKT133 (=IAM 15419 =KCTC 12842). The novel order ord. nov. and family fam. nov. are proposed for the distinct phyletic line represented by the genus .

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2008-03-01
2020-01-23
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