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A novel extremely thermophilic sulfur-oxidizing bacterium, strain LS12-2T, was isolated from a deep-sea hydrothermal field at the Yonaguni Knoll IV, Southern Okinawa Trough. Cells of strain LS12-2T were motile rods, 1.5–4.0 μm in length and 0.4–0.5 μm in width. Strain LS12-2T was an obligate chemolithoautotroph that could utilize elemental sulfur or thiosulfate as an electron donor and nitrate or oxygen as an electron acceptor. Growth was observed at 65–85 °C (optimum 70–75 °C), pH 5.8–8.3 (optimum pH 6.9–7.5), 1.0–4.0 % (w/v) NaCl (optimum 2.5 %) and 1.0–7.0 % O2 in the gas phase (optimum 3.0 %). Fatty acids detected were C16 : 0 (8.0 %), C18 : 0 (9.0 %), C18 : 1 (62.5 %) and C20 : 1 (20.5 %). The genomic DNA G+C content was 51.3 mol%. 16S rRNA gene sequence analysis indicated that strain LS12-2T belonged to the genus Hydrogenivirga. Based on physiological and phylogenetic characteristics of the isolate, it is proposed that this strain represents a novel species in the genus Hydrogenivirga, Hydrogenivirga okinawensis sp. nov. The type strain of Hydrogenivirga okinawensis is LS12-2T (=JCM 13302T=DSM 17378T).
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International Journal of Systematic and Evolutionary Microbiology vol. 58 , part 3, pp. 676 - 681
Supplementary Fig. S1. Electron micrograph of negatively stained cell of strain LS12-2 T.
Supplementary Fig. S2. The effect of temperature, pH, NaCl and O 2on growth of strain LS12-2 T.
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