1887

International Journal of Systematic and Evolutionary Microbiology

Volume 58, Issue 3

sp. nov., a thermophilic sulfur-oxidizing chemolithoautotroph isolated from a deep-sea hydrothermal field, Southern Okinawa Trough Free

Abstract

A novel extremely thermophilic sulfur-oxidizing bacterium, strain LS12-2, was isolated from a deep-sea hydrothermal field at the Yonaguni Knoll IV, Southern Okinawa Trough. Cells of strain LS12-2 were motile rods, 1.5–4.0 μm in length and 0.4–0.5 μm in width. Strain LS12-2 was an obligate chemolithoautotroph that could utilize elemental sulfur or thiosulfate as an electron donor and nitrate or oxygen as an electron acceptor. Growth was observed at 65–85 °C (optimum 70–75 °C), pH 5.8–8.3 (optimum pH 6.9–7.5), 1.0–4.0 % (w/v) NaCl (optimum 2.5 %) and 1.0–7.0 % O in the gas phase (optimum 3.0 %). Fatty acids detected were C (8.0 %), C (9.0 %), C (62.5 %) and C (20.5 %). The genomic DNA G+C content was 51.3 mol%. 16S rRNA gene sequence analysis indicated that strain LS12-2 belonged to the genus . Based on physiological and phylogenetic characteristics of the isolate, it is proposed that this strain represents a novel species in the genus , sp. nov. The type strain of is LS12-2 (=JCM 13302=DSM 17378).

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2008-03-01
2020-04-01
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Hydrogenivirga okinawensis sp. nov., a thermophilic sulfur-oxidizing chemolithoautotroph isolated from a deep-sea hydrothermal field, Southern Okinawa Trough
Int J Syst Evol Microbiol 58, 676 (2008); https://doi.org/10.1099/ijs.0.64615-0
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vol. , part 3, pp. 676 - 681

Electron micrograph of negatively stained cell of strain LS12-2 .

The effect of temperature, pH, NaCl and O on growth of strain LS12-2 .

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