- Volume 66, Issue 12, 2016
Volume 66, Issue 12, 2016
- New taxa
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- Proteobacteria
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Isolation and characterization of a novel Gram-negative bacterium Chromobacterium alkanivorans sp. nov., strain IITR-71T degrading halogenated alkanes
The taxonomic position of a Gram-stain negative, non-violaceinpigmented bacterium isolated from an insecticide-contaminated site was characterized by a polyphasic approach. The bacterium was able to grow on three different halogenated compounds namely 1-hlorobutane, 1-hloropropane and 1,2-ichloroethane. As a critical step in the degradation of these haloalkanes, stoichiometric amounts of dechlorination were estimated. Based on selective enrichment method for three months, using a highly contaminated mixed chemical soil, a bacterium was obtained and designated as IITR-71T. Its versatility and novelty led us to further characterize it by polyphasic taxonomy. The 16S rRNA gene sequence (1446 bases) comparison showed highest similarity with those of members of the genus Chromobacterium with the most closely related species to strain IITR-71T being Chromobacterium aquaticum (99.3 %) followed by Chromobacterium haemolyticum (98.6 %) and Chromobacterium piscinae (97.1 %). The major ubiquinone was Q-8. Predominant polar lipids are phosphatidylethanolamine (PE), phosphatidylglycerol (PG) and diphosphatidylglycerol (DPG). The DNA G+C content of IITR-71T was estimated to be 61.2 mol%. The genotypic and phenotypic distinctiveness of IITR-71T and its phylogenetic relationships indicate that IITR-71T represents a novel species, for which the name Chromobacterium alkanivorans sp. nov. is proposed. The type strain is IITR-71T (=MTCC 11059T=JCM 30068T=KCTC 52433T).
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Rickettsia amblyommatis sp. nov., a spotted fever group Rickettsia associated with multiple species of Amblyomma ticks in North, Central and South America
In 1973, investigators isolated a rickettsial organism, designated strain WB-8-2T, from an adult Amblyomma americanum tick collected at Land Between the Lakes National Recreation Area, TN, USA. This organism is now recognized as highly prevalent in A. americanum, as well as several other Amblyomma species found throughout the Western hemisphere. It has been suggested that cross-reactivity to WB-8-2T and similar strains contributes to the increasing number of spotted fever cases reported in the USA. In 1995, investigators provided preliminary evidence that this strain, as well as another strain from Missouri, represented a distinct taxonomic unit within the genus Rickettsia by evaluating sequences of the 16S rRNA and 17 kDa protein genes. However, the bacterium was never formally named, despite the use of the designation ‘ Rickettsia amblyommii’ and later ‘Candidatus Rickettsia amblyommii’, for more than 20 years in the scientific literature. Herein, we provide additional molecular evidence to identify strain WB-8-2T as a representative strain of a unique rickettsial species and present a formal description for the species, with the proposed name modified to Rickettsia amblyommatis sp. nov. to conform to the International Code of Nomenclature of Prokaryotes. We also establish a pure culture of strain WB-8-2T and designate it as the type strain for the species. The type strain is WB-8-2T (=CRIRC RAM004T=CSURP2882T).
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Piscinibacterium candidicorallinum gen. nov., sp. nov., a member of the order Burkholderiales isolated from a fish pond
More LessA bacterial strain designated LYH-15T was isolated from a freshwater fish pond in Taiwan and characterized using a polyphasic taxonomy approach. Cells of LYH-15T were Gram-staining-negative, aerobic, motile by means of a single polar flagellum, poly-β-hydroxybutyrate-containing, non-spore forming, straight rods and formed light-coral-colored colonies. Growth occurred at 15–40 °C (optimum, 30 °C), at pH 5.0–9.0 (optimum, pH 7.0) and with 0–0.5 % NaCl (optimum, 0 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that LYH-15T forms a distinct phyletic line within the order Burkholderiales , with less than 94 % sequence similarity to its closest relatives with validly published names. The predominant fatty acids were summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), C16 : 0 and C18 : 1ω7c. The major isoprenoid quinone was Q-8 and the DNA G+C content was 63.8 mol%. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and several uncharacterized lipids. The major polyamines were 2-hydroxyputrescine and putrescine. On the basis of the genotypic and phenotypic data, LYH-15T represents a novel species of a new genus in the order Burkholderiales , for which the name Piscinibacterium candidicorallinum gen. nov., sp. nov. is proposed. The type strain is LYH-15T (=BCRC 80969T=LMG 29480T=KCTC 52168T).
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Ensifer alkalisoli sp. nov. isolated from root nodules of Sesbania cannabina grown in saline–alkaline soils
Yan Li, Jun Yan, Bing Yu, En Tao Wang, Xiangyue Li, Hui Yan, Wei Liu and Zhihong XieA group of Sesbania cannabina rhizobia belonging to four recA genotypes of a novel group was further characterized in comparison with the related Ensifer species. They showed 98.2 to 99.9 % similarities among themselves and 92.9 to 93.3 % similarities with the most related strain Ensifer sojae CCBAU 05684T in multilocus sequence analysis of recA, atpD and glnII. The genome average nucleotide identity values between representative strain YIC4027T and the type strains of its closely related species were 81.6 to 88.9 %. Identical symbiotic gene (nodA, nodC and nifH) sequences highly similar with those in other Sesbania-nodulating strains ( Rhizobium sp. SIN-1, Neorhizobium huautlense S02T, Ensifer saheli ORS609T and Rhizobium sp. IRBG74) were detected. The representative strain YIC4027T could form effective nodules on its original host Sesbania cannabina, but not on Sophora flavescens, Trifolium repens, Glycine max, Glycyrrhiza uralensis, Phaseolus vulgaris or Medicago sativa. The use of lactulose as sole carbon source, possession of C13 : 0 2-OH, C13 : 1 at 12–13, C15 : 1 iso ω9c, C17 : 0 anteiso and C18 : 0 iso and absence of C14 : 0 anteiso, C15 : 0 anteiso and C18 : 0 3-OH in fatty acids distinguished the strain YIC4027T from the type strains of its closely related species. Based on all the analyses mentioned above, we propose a novel species Ensifer alkalisoli sp. nov. and designate YIC4027T (=HAMBI 3655T=LMG 29286T) as the type strain. The genome size of YIC4027T is 5.97 Mbp, comprising 5588 predicted genes, and the DNA G+C content is 62.2 mol%.
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Sphingomonas piscinae sp. nov., isolated from a fish pond
More LessA bacterial strain designated LYH-20T was isolated from a fish pond in Taiwan and characterized using a polyphasic taxonomy approach. Cells of strain LYH-20T were aerobic, Gram-stain-negative, non-motile, poly-β-hydroxybutyrate containing, showing straight and rod shaped that were covered by large capsules and formed yellow-coloured colonies. Growth occurred at 15–40 °C (optimum, 30 °C), with 0–1.0 % NaCl (optimum, 0–0.1 %) and at pH 5.0–9.0 (optimum, pH 8.0–9.0). According to a phylogenetic tree based on 16S rRNA gene sequence analysis, strain LYH-20T belonged to the genus Sphingomonas and clustered with Sphingomonas fonticola TNR-2T, with which it shared the highest 16S rRNA gene sequence similarity (97.5 %). The major fatty acids (>10 %) of strain LYH-20T were C18 : 1ω7c and C16 : 0. The DNA G+C content was 67.5 mol%. The sole isoprenoid quinone was Q-10. The polyamines detected were spermidine, putrescine and homospermidine. The polar lipid profile consisted of a mixture of sphingoglycolipid, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol and three uncharacterized phospholipids. The DNA–DNA hybridization value for strain LYH-20T with Sphingomonas fonticola TNR-2T was less than 35 %. Phenotypic characteristics of the novel strain also differed from those of the closest related species of the genus Sphingomonas . On the basis of the genotypic, chemotaxonomic and phenotypic data, strain LYH-20T represents a novel species in the genus Sphingomonas , for which the name Sphingomonas piscinae sp. nov. is proposed. The type strain is LYH-20T (=BCRC 80911T=LMG 29002T=KCTC 42741T).
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Microvirgula curvata sp. nov., isolated from hydrocarbon-contaminated soil, and emended description of the genus Microvirgula
More LessA novel Gram-stain-negative, small curved-rod-shaped, motile strain, designated L6T, was isolated from hydrocarbon-contaminated soils collected from Kuwait. Strain L6T was able to grow at 10–40 °C (optimum, 27–32 °C), pH 6.1–8.8 (optimum, 6.5–7.5) and 0–4.5 % (w/v) NaCl (optimum, 0–0.5). C18 : 1ω6c/C18 : 1ω7c, C16 : 0, C16 : 1ω6c/C16 : 1ω7c, C12 : 0 and C12 : 0 3-OH were predominant fatty acids with minor amounts of C14 : 0 and C17 : 0 cyclo. Phosphatidylglycerol and phosphatidylethanolamine were major polar lipids. The genomic G+C content was 61.2 mol%. 16S rRNA gene sequence comparisons indicated that strain L6T represents a member of the genus Microvirgula within the family Neisseriaceae of the class Betaproteobacteria . Strain L6T has a sequence similarity of 99.2 % with Microvirgula aerodenitrificans SGLY2T and <93.8 % with other members of the family Neisseriaceae . However, strain L6T showed only 56.5±2 % relatedness (based on DNA–DNA hybridization) with M. aerodenitrificans KACC 12055T (=SGLY2T). Distinct morphological, physiological and genotypic differences from the previously described taxa support the classification of strain L6T as a representative of a novel species in the genus Microvirgula , for which the name Microvirgula curvata sp. nov. is proposed. The type strain is L6T (=KEMB 2255-471T=JCM 31223T). An emended description of the genus Microvirgula is also proposed.
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Transfer of the potato plant isolates of Pectobacterium wasabiae to Pectobacterium parmentieri sp. nov.
Pectobacterium wasabiae was originally isolated from Japanese horseradish (Eutrema wasabi), but recently some Pectobacterium isolates collected from potato plants and tubers displaying blackleg and soft rot symptoms were also assigned to P. wasabiae . Here, combining genomic and phenotypical data, we re-evaluated their taxonomic position. PacBio and Illumina technologies were used to complete the genome sequences of P. wasabiae CFBP 3304T and RNS 08-42-1A. Multi-locus sequence analysis showed that the P. wasabiae strains RNS 08-42-1A, SCC3193, CFIA1002 and WPP163, which were collected from potato plant environment, constituted a separate clade from the original Japanese horseradish P. wasabiae . The taxonomic position of these strains was also supported by calculation of the in-silico DNA–DNA hybridization, genome average nucleotide indentity, alignment fraction and average nucleotide indentity values. In addition, they were phenotypically distinguished from P. wasabiae strains by producing acids from (+)-raffinose, α-d(+)-α-lactose, d(+)-galactose and (+)-melibiose but not from methyl α-d-glycopyranoside, (+)-maltose or malonic acid. The name Pectobacterium parmentieri sp. nov. is proposed for this taxon; the type strain is RNS 08-42-1AT (=CFBP 8475T=LMG 29774T).
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Idiomarina tyrosinivorans sp. nov., isolated from estuarine surface water
A tyrosine-metabolizing, Gram-stain-negative, strictly aerobic, non-spore-forming, curved-rod-shaped, motile (due to monopolar flagellum) marine bacterium, designated strain CC-PW-9T, was isolated from estuarine water off Pingtung, Taiwan. Strain CC-PW-9T not only shared highest 16S rRNA gene sequence similarities with Idiomarina representatives (96.4–93.4 %, n=26), but also formed a distinct phyletic lineage and coherent phylogenetic cluster associated with those species. Cells of strain CC-PW-9T grew with 6–12 % (w/v) NaCl, at 20–40 °C and at pH 6–9. It produced predominant amounts of phosphatidylethanolamine, plus diphosphatidylglycerol, phosphatidylglycerol, phosphatidylserine, two unidentified phospholipids, an unidentified phosphoglycolipid, two unidentified lipids and an unidentified aminolipid in moderate to trace amounts. Fatty acids such as iso-C15 : 0, iso-C17 : 0 and iso-C17 : 1 ω9c and/or C16 : 010-methyl (summed feature 9) were found in major amounts. The DNA G+C content was 51.1 mol%. Ubiquinone-8 (Q-8) was the sole respiratory quinone. Based on evidence from this polyphasic taxonomic study, strain CC-PW-9T is considered to represent a novel species of the genus Idiomarina , affiliated to the family Idiomarinaceae , for which the name Idiomarina tyrosinivorans sp. nov. is proposed. The type strain is CC-PW-9T (=JCM 19757T=BCRC 80745T).
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Acinetobacter celticus sp. nov., a psychrotolerant species widespread in natural soil and water ecosystems
More LessA novel, taxonomically unique group of six strains of the genus Acinetobacter was discovered during an exploratory study on strains culturable from soil and water natural ecosystems in the Bohemian part of the Czech Republic. Based on the comparative analyses of the 16S rRNA gene, gyrB and rpoB sequences, these strains formed strongly supported and internally coherent clusters (intracluster identities of ≥99.9, ≥96.1 and ≥97.3 %, respectively), which were clearly separated from all known species of the genus Acinetobacter (≤98.7, ≤83.2 and ≤88.9 %, respectively). The distinctness of the group at the species level was evidenced also by the results of the genus-wide analyses of the whole-cell mass fingerprints of the six strains generated by matrix-assisted laser desorption/ionization-time-of-flight MS and the whole-genome sequence of a group member, ANC 4603T. Compared with the known species of the genus Acinetobacter , all six strains exhibited a unique phenotype, characterized by psychrotolerance (growth at 1 °C through 28 °C), the inability to grow at 32 °C and the ability to assimilate l-aspartate and malonate but not 2,3-butanediol or citrate. Based on these results, the name Acinetobacter celticus sp. nov. is proposed for the taxon represented by the six strains. The type strain is ANC 4603T (=CCM 8700T=CCUG 69239T=CNCTC 7549T).
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Gilvimarinus japonicus sp. nov., a cellulolytic and agarolytic marine bacterium isolated from coastal debris
A cellulolytic and agarolytic bacterial strain, designated 12-2T, was isolated from a piece of cotton rope fragment washed ashore on a beach and was studied phenotypically, genotypically and phylogenetically. Analyses of 16S rRNA and gyrB gene sequences and DNA base composition suggested that the strain is a member of the genus Gilvimarinus . However, levels of 16S rRNA and gyrB gene sequence similarity between it and the type strains of Gilvimarinus species were no higher than 97.9 and 78.7 %, respectively, suggesting that the strain is distinct. Moreover, the results of DNA–DNA hybridization experiments and physiological characterization clearly differentiated the strain from its closest neighbours. The strain is therefore considered to represent a novel species of the genus Gilvimarinus , for which the name Gilvimarinus japonicus sp. nov. is proposed. The type strain is 12-2T (=NBRC 111987T=KCTC 52141T).
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Altererythrobacter sediminis sp. nov., isolated from lagoon sediments
More LessA Gram-stain-negative, non-motile, non-spore-forming, ovoid rod-shaped bacterium, designated strain CAU1172T, was isolated from lagoon sediments along the east coast of the Republic of Korea. Strain CAU1172T formed a yellow pigment on marine agar. Growth occurred at 20–37 °C (optimum, 30 °C), at pH 6.5–10 (optimum, 7.5) and in the presence of 0–4 % (w/v) NaCl (optimum, 1 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CAU1172T formed a separate lineage within the genus Altererythrobacter , and was most closely related to Altererythrobacter gangjinensis KJ7T (96.1 % similarity). Ubiquinone 10 (Q-10) was the predominant respiratory quinone. The dominant fatty acids were C18 : 1 ω7c, C17 : 1 ω6c and summed feature 3 (comprising C16 : 1 ω7c and/or C16 : 1 ω6c). The polar lipids were composed of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, sphingoglycolipid, phospatidylcholine and four unidentified lipids. The DNA G+C content of strain CAU1172T was 63.2 mol%. On the basis of phenotypic and chemotaxonomic data, strain CAU1172T represents a novel species of the genus Altererythrobacter , for which the name Altererythrobacter sediminis sp. nov. is proposed. The type strain is CAU1172T (=KCTC 42453T=NBRC 110917T).
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Luteimonas padinae sp. nov., an epiphytic bacterium isolated from an intertidal macroalga
A Gram-stain-negative, rod-shaped bacterium, forming yellow colonies and designated CDR SL 15T, was isolated from the surface of Padina sp., a brown macroalga, which grows in the Western coastal regions of the state of Goa, India. The 16S rRNA gene sequence phylogeny placed the strain in the genus Luteimonas and it showed closest sequence similarity to Luteimonas terricola BZ92rT (97.6 %) and <97.0 % to other species of the genus Luteimonas . Chemotaxonomic features, such as having iso-C15 : 0 and summed feature 9 (C16 : 0 10-methyl/iso-C17 : 1ω9c) as the major fatty acids and Q-8 as the only ubiquinone further supported its placement within this genus. There were some critical differences in phenotypic properties between Luteimonas padinae sp. nov. CDR SL 15T and L. terricola DSM 22344T i.e. temperature range for growth and salinity range and optimum for growth ( L. terricola is a psychrotolerant bacterium with a lower optimum temperature for growth), acid production and assimilation of substrates, enzyme activities and resistance to certain antibiotics. The DNA–DNA relatedness value of the novel strain with its closest phylogenetic relative was only 40 %, below the 70 % threshold value recommended for species delineation. All these characteristics are consistent with strain CDR SL 15T representing a novel species of the genus Luteimonas , for which the name Luteimonas padinae sp. nov. is proposed. The type strain is CDR SL 15T (=DSM 101536T=KCTC 52403T).
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Identification and taxonomic characterization of Bordetella pseudohinzii sp. nov. isolated from laboratory-raised mice
Bordetella hinzii is known to cause respiratory disease in poultry and has been associated with a variety of infections in immunocompromised humans. In addition, there are several reports of B. hinzii infections in laboratory-raised mice. Here we sequenced and analysed the complete genome sequences of multiple B. hinzii -like isolates, obtained from vendor-supplied C57BL/6 mice in animal research facilities on different continents, and we determined their taxonomic relationship to other Bordetella species. The whole-genome based and 16S rRNA gene based phylogenies each identified two separate clades in B. hinzii , one was composed of strains isolated from poultry, humans and a rabbit whereas the other clade was restricted to isolates from mice. Distinctly different estimated DNA–DNA hybridization values, average nucleotide identity scores, gene content, metabolic profiles and host specificity all provide compelling evidence for delineation of the two species, B. hinzii – from poultry, humans and rabbit – and Bordetella pseudohinzii sp. nov. type strain 8-296-03T (=NRRL B-59942T=NCTC 13808T) that infect mice.
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Arsenicitalea aurantiaca gen. nov., sp. nov., a new member of the family Hyphomicrobiaceae, isolated from high-arsenic sediment
A novel arsenic-resistant bacterium, designated 42-50T, was isolated from the high-arsenic sediment of Jianghan Plain, Hubei Province, China. Phylogenetic and biochemical analysis indicated that this bacterium represents the first species of a novel genus belonging to the family Hyphomicrobiaceae . The 16S rRNA gene of strain 42-50T shares 96.3–94.2, 96.3, 96.2 and 94.9–93.8 % sequence identities to those of species from the genera Devosia , Youhaiella , Paradevosia and Pelagibacterium , respectively. The major cellular fatty acids are C16 : 0, C18 : 0, C18 : 1 ω7c 11-methyl and summed feature 8 (comprising C18 : 1 ω7c and C18 : 1 ω6c). The predominant polar lipids are diphosphatidylglycerol, phosphatidylglycerol and two unidentified glycolipids. The predominant respiratory quinone is ubiquinone-10 (Q-10). The DNA G+C content of strain 42-50T is 73.7 mol%. The distinct phylogenetic lineage and unique cellular fatty acids suggest that strain 42-50T represents a novel species of a new genus affiliated with the family Hyphomicrobiaceae , for which the name Arsenicitalea aurantiaca gen. nov., sp. nov. is proposed. The type strain is 42-50T (=CCTCC AB 2014325T=KCTC 42825T).
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Sphingomonas lutea sp. nov., isolated from freshwater of an artificial reservoir
More LessAn aerobic, Gram-stain-negative, gliding and yellow-pigmented bacterium, designated strain JS5T, was isolated from freshwater of Juam reservoir, Republic of Korea. Cells were catalase-positive and oxidase-negative. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain JS5T forms an independent lineage within the genus Sphingomonas . Strain JS5T was related distantly to ‘ Sphingomonas parvus ’ GP20-2 (98.2 % 16S rRNA gene sequence similarity), Sphingomonas sediminicola Dae 20T (96.8 %) and Sphingomonas daechungensis CH15-11T (96.7 %). The major fatty acids of strain JS5T were C16 : 0, summed feature 3 comprising C16 : 1 ω7c and/or C16 : 1 ω6c and summed feature 8 comprising C18 : 1 ω7c and/or C18 : 1 ω6c. The predominant isoprenoid quinone of the isolate was ubiquinone-10. The DNA G+C content of strain JS5T was 65 mol%. Phenotypic characteristics distinguished strain JS5T from related species of the genus Sphingomonas . On the basis of the evidence presented in this study, a novel species, Sphingomonas lutea sp. nov., is proposed to accommodate strain JS5T (=KCTC 23642T=JCM 18309T).
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Tropicimonas arenosa sp. nov., isolated from marine sand
More LessA Gram-staining-negative, aerobic, non-motile, rod-shaped bacterial strain, designated CAU 1062T, was isolated from marine sand in Jeju island, Republic of Korea and its taxonomic position was investigated using a polyphasic approach. CAU 1062T grew optimally at 30 °C and pH 8 in the presence of 2 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that CAU 1062T formed a distinct lineage within the genus Tropicimonas and was the most closely related to Tropicimonas sediminicola M97T (similarity 96.11 %). The strain had ubiquinone-10 (Q-10) as the predominant respiratory quinone and C18 : 1ω7c as the major cellular fatty acid. The polar lipid pattern of CAU 1062T consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, an aminolipid, six phospholipids and five lipids. The DNA G+C content was 65.7 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, CAU 1062T represents a novel species of the genus Tropicimonas , for which the name Tropicimonas arenosa sp. nov. is proposed. The type strain is CAU 1062T (=KCTC 52178T=NBRC 111995T).
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Marinomonas blandensis sp. nov., a novel marine gammaproteobacterium
A novel Gram-staining-negative, chemoorganotrophic, moderately halophilic, strictly aerobic bacterium, strain MED121T, was isolated from a seawater sample collected at the Blanes Bay Microbial Observatory in the north-western Mediterranean Sea. Analysis of its 16S rRNA gene sequence, retrieved from the whole-genome sequence, showed that this bacterium was most closely related to Marinomonas dokdonensis and other Marinomonas species (96.3 and 93.3–95.7 % sequence similarities, respectively), within the family Oceanospirillaceae . Strain MED121T was included into a whole-genome sequencing study and, subsequently, it was characterized using a polyphasic taxonomic approach. It was found to be oxidase and catalase positive, its cells are cocci to short rods, it does not ferment carbohydrates and does not reduce nitrate to nitrite or gas and it requires at least 2.5 % (w/v) marine salts and tolerates up to 7 % (w/v) salts. Its major cellular fatty acids in order of abundance are C16 : 1ω7c/C16 : 1ω6c, C18 : 1ω7c, C16 : 0 and C10 : 0 3-OH. Its genome had an approximate length of 5.1 million bases and a DNA G+C content equal to 40.9 mol%. Analysis of the annotated genes reveals the capacity for the synthesis of ubiquinone 8 (Q8) and the polar lipids phosphatidylglycerol and phosphatidylethanolamine, in agreement with other members of the genus. All the data collected supported the creation of a novel species to accommodate this bacterium, for which the name Marinomonas blandensis sp. nov. is proposed. The type strain is MED121T (=CECT 7076T=LMG 29722T).
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Primorskyibacter aestuariivivens sp. nov., isolated from a tidal flat, and emended description of the genus Primorskyibacter
More LessA Gram-stain-negative, aerobic, non-spore-forming, motile and coccoid, ovoid or rod-shaped bacterial strain, designated OITF-36T, was isolated from a tidal flat in Oido, an island of South Korea, and subjected to a polyphasic taxonomic study. Strain OITF-36T grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0–3.0 % (w/v) NaCl. Neighbour-joining and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences revealed that strain OITF-36T clustered with the type strains of Primorskyibacter sedentarius and Primorskyibacter insulae , showing 97.4–97.6 % sequence similarity. It exhibited sequence similarity values of less than 96.9 % to the type strains of the other recognized species. Strain OITF-36T contained Q-10 as the predominant ubiquinone and C18 : 1 ω7c as the major fatty acid. The major polar lipids detected in strain OITF-36T were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, one unidentified aminolipid and one unidentified lipid. The DNA G+C content of strain OITF-36T was 61.8 mol%, and its mean DNA–DNA relatedness values with the type strains of P . sedentarius and P . insulae were 16–24 %. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain OITF-36T is separate from P . sedentarius and P . insulae . On the basis of the data presented, strain OITF-36T is considered to represent a novel species of the genus Primorskyibacter , for which the name Primorskyibacter aestuariivivens sp. nov. is proposed. The type strain is OITF-36T (=KCTC 52455T=NBRC 112419T).
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- Eukaryotic micro-organisms
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Apiotrichum terrigenum sp. nov., a soil-associated yeast found in both the UK and mainland Europe
Five arthroconidium-producing yeast strains representing a novel Trichosporon-like species were independently isolated from the UK, Hungary and Norway. Two strains (Bio4T and Bio21) were isolated from biogas reactors used for processing grass silage, with a third strain (S8) was isolated from soil collected at the same UK site. Two additional strains were isolated in mainland Europe, one from soil in Norway (NCAIM Y.02175) and the other from sewage in Hungary (NCAIM Y.02176). Sequence analyses of the D1/D2 domains of the LSU rRNA gene and internal transcribed spacer (ITS) region indicated that the novel species belongs to the recently reinstated genus Apiotrichum and is most closely related to Apiotrichum scarabaeorum, a beetle-associated species first found in South Africa. Despite having similar physiological characteristics, the two species can be readily distinguished from one another by ITS sequencing. The species name Apiotrichum terrigenum sp. nov. is proposed to accommodate these strains, with Bio4T (=CBS 11373T=NCYC 3540T) designated as the type strain. The Mycobank deposit number is MB817431.
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Kockovaella libkindii sp. nov., a yeast species isolated from water tanks of bromeliad
During a study of yeast community associated with water tanks (phytotelmata) of the bromeliad Vriesea minarum, two strains of a novel stalk-forming yeast species were found. The sequences of the region spanning the ITS and D1/D2 domains of the large subunit rRNA gene showed that this species belongs to the genus Kockovaella. The novel species differs by 14 or more nucleotide substitutions in the D1/D2 domains and by 26 or more substitutions in the ITS–5.8S region from all other Kockovaella species. We describe this species as Kockovaella libkindii sp. nov. The type strain of Kockovaella libkindii sp. nov. is UFMG-CM-Y6053T (=UFMG-BRO-488T=CBS 12685T). The MycoBank number is MB 817710.
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Volume 24 (1974)
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Volume 23 (1973)
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Volume 22 (1972)
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Volume 21 (1971)
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Volume 20 (1970)
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Volume 19 (1969)
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Volume 18 (1968)
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Volume 17 (1967)
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Volume 16 (1966)
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Volume 15 (1965)
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Volume 14 (1964)
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Volume 13 (1963)
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Volume 12 (1962)
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Volume 11 (1961)
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Volume 10 (1960)
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Volume 9 (1959)
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Volume 8 (1958)
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Volume 7 (1957)
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Volume 6 (1956)
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Volume 5 (1955)
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Volume 4 (1954)
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Volume 3 (1953)
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Volume 2 (1952)
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Volume 1 (1951)