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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 66, Issue 12

sp. nov., an epiphytic bacterium isolated from an intertidal macroalga Free

Abstract

A Gram-stain-negative, rod-shaped bacterium, forming yellow colonies and designated CDR SL 15, was isolated from the surface of sp., a brown macroalga, which grows in the Western coastal regions of the state of Goa, India. The 16S rRNA gene sequence phylogeny placed the strain in the genus and it showed closest sequence similarity to BZ92r (97.6 %) and <97.0 % to other species of the genus . Chemotaxonomic features, such as having iso-C and summed feature 9 (C 10-methyl/iso-Cω9) as the major fatty acids and Q-8 as the only ubiquinone further supported its placement within this genus. There were some critical differences in phenotypic properties between sp. nov. CDR SL 15 and DSM 22344 i.e. temperature range for growth and salinity range and optimum for growth ( is a psychrotolerant bacterium with a lower optimum temperature for growth), acid production and assimilation of substrates, enzyme activities and resistance to certain antibiotics. The DNA–DNA relatedness value of the novel strain with its closest phylogenetic relative was only 40 %, below the 70 % threshold value recommended for species delineation. All these characteristics are consistent with strain CDR SL 15 representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CDR SL 15 (=DSM 101536=KCTC 52403).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA , CDR-SL15T , FAME , MALDI-TOF and phylogeny
© 2016 IUMS
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2016-12-01
2024-04-19
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References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. 1990; Basic local alignment search tool. J Mol Biol 215:403–410 [View Article]
     [Google Scholar]
  2. Baik K. S., Park S. C., Kim M. S., Kim E. M., Park C., Chun J., Seong C. N. 2008; Luteimonas marina sp. nov., isolated from seawater. Int J Syst Evol Microbiol 58:2904–2908 [View Article][PubMed]
     [Google Scholar]
  3. Bligh E. G., Dyer W. J. 1959; A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917 [CrossRef]
     [Google Scholar]
  4. Bowman J. P., McCammon S. A., Brown J. L., Nichols P. D., McMeekin T. A. 1997; Psychroserpens burtonensis gen. nov., sp. nov., and Gelidibacter algens gen. nov., sp. nov., psychrophilic bacteria isolated from Antarctic lacustrine and sea ice habitats. Int J Syst Bacteriol 47:670–677 [View Article][PubMed]
     [Google Scholar]
  5. Chou J. H., Cho N. T., Arun A. B., Young C. C., Chen W. M. 2008; Luteimonas aquatica sp. nov., isolated from fresh water from Southern Taiwan. Int J Syst Evol Microbiol 58:2051–2055 [View Article][PubMed]
     [Google Scholar]
  6. Fan X., Yu T., Li Z., Zhang X. H. 2014; Luteimonas abyssi sp. nov., isolated from deep-sea sediment. Int J Syst Evol Microbiol 64:668–674 [View Article][PubMed]
     [Google Scholar]
  7. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
     [Google Scholar]
  8. Finkmann W., Altendorf K., Stackebrandt E., Lipski A. 2000; Characterization of N2O-producing Xanthomonas-like isolates from biofilters as Stenotrophomonas nitritireducens sp. nov., Luteimonas mephitis gen. nov., sp. nov. and Pseudoxanthomonas broegbernensis gen. nov., sp. nov. Int J Syst Evol Microbiol 50:273–282 [View Article][PubMed]
     [Google Scholar]
  9. Kester D. R., Duedall I. W., Connors D. N., Pytkowicz R. M. 1967; Preparation of artificial sea water. Limnol & Oceanogr 12:176–179 [CrossRef]
     [Google Scholar]
  10. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
     [Google Scholar]
  11. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
     [Google Scholar]
  12. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207 [CrossRef]
     [Google Scholar]
  13. Krishnamurthi S., Bhattacharya A., Mayilraj S., Saha P., Schumann P., Chakrabarti T. 2009; Description of Paenisporosarcina quisquiliarum gen. nov., sp. nov., and reclassification of Sporosarcina macmurdoensis Reddy et al. 2003 as Paenisporosarcina macmurdoensis comb. nov. Int J Syst Evol Microbiol 59:1364–1370 [View Article][PubMed]
     [Google Scholar]
  14. Lányi B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67 [CrossRef]
     [Google Scholar]
  15. Mandel M., Marmur J. 1968; Use of ultraviolet absorbance temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol 12B:195–206 [CrossRef]
     [Google Scholar]
  16. Mu Y., Pan Y., Shi W., Liu L., Jiang Z., Luo X., Zeng X. C., Li W. J. 2016; Luteimonas arsenica sp. nov., a new arsenic-tolerant bacterium isolated from arsenic-contaminated soil. Int J Syst Evol Microbiol 66:2291–2296 [View Article][PubMed]
     [Google Scholar]
  17. Nei M., Kumar S. 2000 Molecular Evolution and Phylogenetics New York: Oxford University Press;
     [Google Scholar]
  18. Pandey K. K., Chakrabarti T., Mayilraj S. 2002; Pseudomonas indica sp. nov., a novel butane-utilizing species. Int J Syst Evol Microbiol 52:1559–1567 [View Article]
     [Google Scholar]
  19. Park Y. J., Park M. S., Lee S. H., Park W., Lee K., Jeon C. O. 2011; Luteimonas lutimaris sp. nov., isolated from a tidal flat. Int J Syst Evol Microbiol 61:2729–2733 [View Article][PubMed]
     [Google Scholar]
  20. Pitcher D. G., Saunders N. A., Owen R. J. 1989; Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 8:151–156 [View Article]
     [Google Scholar]
  21. Rani P., Mukherjee U., Verma H., Kamra K., Lal R. 2016; Luteimonas tolerans sp. nov., isolated from hexachlorocyclohexane-contaminated soil. Int J Syst Evol Microbiol 66:1851–1856 [View Article][PubMed]
     [Google Scholar]
  22. Reddy G. S. N., Matsumuto G. I., Shivaji S. 2003; Sporosarcina macmurdoensis sp. nov., from a cyanobacterial mat sample from a pond in the Mcmurdo Dry Valleys, Antarctica. Int J Syst Evol Microbiol 53:1363–1367 [View Article][PubMed]
     [Google Scholar]
  23. Roh S. W., Kim K. H., Nam Y. D., Chang H. W., Kim M. S., Yoon J. H., Oh H. M., Bae J. W. 2008; Luteimonas aestuarii sp. nov., isolated from tidal flat sediment. J Microbiol 46:525–529 [View Article][PubMed]
     [Google Scholar]
  24. Romanenko L. A., Tanaka N., Svetashev V. I., Kurilenko V. V., Mikhailov V. V. 2013; Luteimonas vadosa sp. nov., isolated from seashore sediment. Int J Syst Evol Microbiol 63:1261–1266 [View Article][PubMed]
     [Google Scholar]
  25. Saddler G. S., Bradbury J. F. 2005; Family I. Xanthomonadaceae . In Bergey’s Manual of Systematic Bacteriology, 2nd edn. vol. 2 pp 459–462 Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. New York: Springer;
     [Google Scholar]
  26. Saha P., Mondal A. K., Mayilraj S., Krishnamurthi S., Bhattacharya A., Chakrabarti T. 2005; Paenibacillus assamensis sp. nov., a novel bacterium isolated from a warm spring in Assam, India. Int J Syst Evol Microbiol 55:2577–2581 [View Article][PubMed]
     [Google Scholar]
  27. Saitou N., Nei M. 1987; The neighbour-joining method a new method for reconstructing phylogenetic trees. Mol Bio Evol 4:406–425
     [Google Scholar]
  28. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
     [Google Scholar]
  29. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Evol Microbiol 44:846–849 [View Article]
     [Google Scholar]
  30. Sun Z. B., Zhang H., Yuan X. F., Wang Y. X, Feng D. M., Wang Y. H., Feng Y. J. 2012; Luteimonas cucumeris sp. nov., isolated from a cucumber leaf. Int J Syst Evol Microbiol 62:2916–2920 [View Article][PubMed]
     [Google Scholar]
  31. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013; mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729 [View Article][PubMed]
     [Google Scholar]
  32. Ten L. N., Jung H. M., Im W. T., Yoo S. A., Oh H. M., Lee S. T. 2009; Lysobacter panaciterrae sp. nov., isolated from soil of a ginseng field. Int J Syst Evol Microbiol 59:958–963 [View Article][PubMed]
     [Google Scholar]
  33. Tourova T. P., Antonov A. S. 1987; Identification of microorganisms by rapid DNA-DNA hybridization. Methods Microbiol 19:333–335 [CrossRef]
     [Google Scholar]
  34. Verma A., Mual P., Mayilraj S., Krishnamurthi S. 2015; Tamilnaduibacter salinus gen. nov., sp. nov., a halotolerant gammaproteobacterium within the family Alteromonadaceae, isolated from a salt pan in Tamilnadu, India. Int J Syst Evol Microbiol 65:3248–3255 [View Article][PubMed]
     [Google Scholar]
  35. Wu G., Liu Y., Li Q., Du H., You J., Li H., Ke C., Zhang X., Yu J., Zhao T. 2013; Luteimonas huabeiensis sp. nov., isolated from stratum water. Int J Syst Evol Microbiol 63:3352–3357 [View Article][PubMed]
     [Google Scholar]
  36. Young C. C., Kämpfer P., Chen W. M., Yen W. S., Arun A. B., Lai W. A., Shen F. T., Rekha P. D., Lin K. Y., Chou J. H. 2007; Luteimonas composti sp. nov., a moderately thermophilic bacterium isolated from food waste. Int J Syst Evol Microbiol 57:741–744 [View Article][PubMed]
     [Google Scholar]
  37. Zhang D. C., Liu H. C., Xin Y. H., Zhou Y. G., Schinner F., Margesin R. 2010; Luteimonas terricola sp. nov., a psychrophilic bacterium isolated from soil. Int J Syst Evol Microbiol 60:1581–1584 [View Article][PubMed]
     [Google Scholar]
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Luteimonas padinae sp. nov., an epiphytic bacterium isolated from an intertidal macroalga
Int J Syst Evol Microbiol 66, 5444 (2016); https://doi.org/10.1099/ijsem.0.001539
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