1887

Abstract

A Gram-stain-negative, rod-shaped bacterium, forming yellow colonies and designated CDR SL 15, was isolated from the surface of Padina sp., a brown macroalga, which grows in the Western coastal regions of the state of Goa, India. The 16S rRNA gene sequence phylogeny placed the strain in the genus Luteimonas and it showed closest sequence similarity to Luteimonas terricola BZ92r (97.6 %) and <97.0 % to other species of the genus Luteimonas . Chemotaxonomic features, such as having iso-C15 : 0 and summed feature 9 (C16 : 0 10-methyl/iso-C17 : 1ω9c) as the major fatty acids and Q-8 as the only ubiquinone further supported its placement within this genus. There were some critical differences in phenotypic properties between Luteimonas padinae sp. nov. CDR SL 15 and L. terricola DSM 22344 i.e. temperature range for growth and salinity range and optimum for growth ( L. terricola is a psychrotolerant bacterium with a lower optimum temperature for growth), acid production and assimilation of substrates, enzyme activities and resistance to certain antibiotics. The DNA–DNA relatedness value of the novel strain with its closest phylogenetic relative was only 40 %, below the 70 % threshold value recommended for species delineation. All these characteristics are consistent with strain CDR SL 15 representing a novel species of the genus Luteimonas , for which the name Luteimonas padinae sp. nov. is proposed. The type strain is CDR SL 15 (=DSM 101536=KCTC 52403).

Keyword(s): 16S rRNA , CDR-SL15T , FAME , MALDI-TOF and phylogeny
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001539
2016-12-01
2019-09-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/12/5444.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001539&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J..( 1990;). Basic local alignment search tool. . J Mol Biol 215: 403–410. [CrossRef]
    [Google Scholar]
  2. Baik K. S., Park S. C., Kim M. S., Kim E. M., Park C., Chun J., Seong C. N..( 2008;). Luteimonas marina sp. nov., isolated from seawater. . Int J Syst Evol Microbiol 58: 2904–2908. [CrossRef] [PubMed]
    [Google Scholar]
  3. Bligh E. G., Dyer W. J..( 1959;). A rapid method of total lipid extraction and purification. . Can J Biochem Physiol 37: 911–917.[CrossRef]
    [Google Scholar]
  4. Bowman J. P., McCammon S. A., Brown J. L., Nichols P. D., McMeekin T. A..( 1997;). Psychroserpens burtonensis gen. nov., sp. nov., and Gelidibacter algens gen. nov., sp. nov., psychrophilic bacteria isolated from Antarctic lacustrine and sea ice habitats. . Int J Syst Bacteriol 47: 670–677. [CrossRef] [PubMed]
    [Google Scholar]
  5. Chou J. H., Cho N. T., Arun A. B., Young C. C., Chen W. M..( 2008;). Luteimonas aquatica sp. nov., isolated from fresh water from Southern Taiwan. . Int J Syst Evol Microbiol 58: 2051–2055. [CrossRef] [PubMed]
    [Google Scholar]
  6. Fan X., Yu T., Li Z., Zhang X. H..( 2014;). Luteimonas abyssi sp. nov., isolated from deep-sea sediment. . Int J Syst Evol Microbiol 64: 668–674. [CrossRef] [PubMed]
    [Google Scholar]
  7. Felsenstein J..( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39: 783–791. [CrossRef]
    [Google Scholar]
  8. Finkmann W., Altendorf K., Stackebrandt E., Lipski A..( 2000;). Characterization of N2O-producing Xanthomonas-like isolates from biofilters as Stenotrophomonas nitritireducens sp. nov., Luteimonas mephitis gen. nov., sp. nov. and Pseudoxanthomonas broegbernensis gen. nov., sp. nov. . Int J Syst Evol Microbiol 50: 273–282. [CrossRef] [PubMed]
    [Google Scholar]
  9. Kester D. R., Duedall I. W., Connors D. N., Pytkowicz R. M..( 1967;). Preparation of artificial sea water. . Limnol & Oceanogr 12: 176–179.[CrossRef]
    [Google Scholar]
  10. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al.( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62: 716–721. [CrossRef] [PubMed]
    [Google Scholar]
  11. Kimura M..( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16: 111–120. [CrossRef] [PubMed]
    [Google Scholar]
  12. Komagata K., Suzuki K..( 1987;). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19: 161–207.[CrossRef]
    [Google Scholar]
  13. Krishnamurthi S., Bhattacharya A., Mayilraj S., Saha P., Schumann P., Chakrabarti T..( 2009;). Description of Paenisporosarcina quisquiliarum gen. nov., sp. nov., and reclassification of Sporosarcina macmurdoensis Reddy et al. 2003 as Paenisporosarcina macmurdoensis comb. nov. . Int J Syst Evol Microbiol 59: 1364–1370. [CrossRef] [PubMed]
    [Google Scholar]
  14. Lányi B..( 1987;). Classical and rapid identification methods for medically important bacteria. . Methods Microbiol 19: 1–67.[CrossRef]
    [Google Scholar]
  15. Mandel M., Marmur J..( 1968;). Use of ultraviolet absorbance temperature profile for determining the guanine plus cytosine content of DNA. . Methods Enzymol 12B: 195–206.[CrossRef]
    [Google Scholar]
  16. Mu Y., Pan Y., Shi W., Liu L., Jiang Z., Luo X., Zeng X. C., Li W. J..( 2016;). Luteimonas arsenica sp. nov., a new arsenic-tolerant bacterium isolated from arsenic-contaminated soil. . Int J Syst Evol Microbiol 66: 2291–2296. [CrossRef] [PubMed]
    [Google Scholar]
  17. Nei M., Kumar S..( 2000;). Molecular Evolution and Phylogenetics. New York:: Oxford University Press;.
    [Google Scholar]
  18. Pandey K. K., Chakrabarti T., Mayilraj S..( 2002;). Pseudomonas indica sp. nov., a novel butane-utilizing species. . Int J Syst Evol Microbiol 52: 1559–1567. [CrossRef]
    [Google Scholar]
  19. Park Y. J., Park M. S., Lee S. H., Park W., Lee K., Jeon C. O..( 2011;). Luteimonas lutimaris sp. nov., isolated from a tidal flat. . Int J Syst Evol Microbiol 61: 2729–2733. [CrossRef] [PubMed]
    [Google Scholar]
  20. Pitcher D. G., Saunders N. A., Owen R. J..( 1989;). Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. . Lett Appl Microbiol 8: 151–156. [CrossRef]
    [Google Scholar]
  21. Rani P., Mukherjee U., Verma H., Kamra K., Lal R..( 2016;). Luteimonas tolerans sp. nov., isolated from hexachlorocyclohexane-contaminated soil. . Int J Syst Evol Microbiol 66: 1851–1856. [CrossRef] [PubMed]
    [Google Scholar]
  22. Reddy G. S. N., Matsumuto G. I., Shivaji S..( 2003;). Sporosarcina macmurdoensis sp. nov., from a cyanobacterial mat sample from a pond in the Mcmurdo Dry Valleys, Antarctica. . Int J Syst Evol Microbiol 53: 1363–1367. [CrossRef] [PubMed]
    [Google Scholar]
  23. Roh S. W., Kim K. H., Nam Y. D., Chang H. W., Kim M. S., Yoon J. H., Oh H. M., Bae J. W..( 2008;). Luteimonas aestuarii sp. nov., isolated from tidal flat sediment. . J Microbiol 46: 525–529. [CrossRef] [PubMed]
    [Google Scholar]
  24. Romanenko L. A., Tanaka N., Svetashev V. I., Kurilenko V. V., Mikhailov V. V..( 2013;). Luteimonas vadosa sp. nov., isolated from seashore sediment. . Int J Syst Evol Microbiol 63: 1261–1266. [CrossRef] [PubMed]
    [Google Scholar]
  25. Saddler G. S., Bradbury J. F..( 2005;). Family I. Xanthomonadaceae. . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn.,vol. 2 pp. 459–462. Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M.. New York:: Springer;.
    [Google Scholar]
  26. Saha P., Mondal A. K., Mayilraj S., Krishnamurthi S., Bhattacharya A., Chakrabarti T..( 2005;). Paenibacillus assamensis sp. nov., a novel bacterium isolated from a warm spring in Assam, India. . Int J Syst Evol Microbiol 55: 2577–2581. [CrossRef] [PubMed]
    [Google Scholar]
  27. Saitou N., Nei M..( 1987;). The neighbour-joining method a new method for reconstructing phylogenetic trees. . Mol Bio Evol 4: 406–425.
    [Google Scholar]
  28. Smibert R. M., Krieg N. R..( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R.. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  29. Stackebrandt E., Goebel B. M..( 1994;). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Evol Microbiol 44: 846–849. [CrossRef]
    [Google Scholar]
  30. Sun Z. B., Zhang H., Yuan X. F., Wang Y. X, Feng D. M., Wang Y. H., Feng Y. J..( 2012;). Luteimonas cucumeris sp. nov., isolated from a cucumber leaf. . Int J Syst Evol Microbiol 62: 2916–2920. [CrossRef] [PubMed]
    [Google Scholar]
  31. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S..( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. . Mol Biol Evol 30: 2725–2729. [CrossRef] [PubMed]
    [Google Scholar]
  32. Ten L. N., Jung H. M., Im W. T., Yoo S. A., Oh H. M., Lee S. T..( 2009;). Lysobacter panaciterrae sp. nov., isolated from soil of a ginseng field. . Int J Syst Evol Microbiol 59: 958–963. [CrossRef] [PubMed]
    [Google Scholar]
  33. Tourova T. P., Antonov A. S..( 1987;). Identification of microorganisms by rapid DNA-DNA hybridization. . Methods Microbiol 19: 333–335.[CrossRef]
    [Google Scholar]
  34. Verma A., Mual P., Mayilraj S., Krishnamurthi S..( 2015;). Tamilnaduibacter salinus gen. nov., sp. nov., a halotolerant gammaproteobacterium within the family Alteromonadaceae, isolated from a salt pan in Tamilnadu, India. . Int J Syst Evol Microbiol 65: 3248–3255. [CrossRef] [PubMed]
    [Google Scholar]
  35. Wu G., Liu Y., Li Q., Du H., You J., Li H., Ke C., Zhang X., Yu J., Zhao T..( 2013;). Luteimonas huabeiensis sp. nov., isolated from stratum water. . Int J Syst Evol Microbiol 63: 3352–3357. [CrossRef] [PubMed]
    [Google Scholar]
  36. Young C. C., Kämpfer P., Chen W. M., Yen W. S., Arun A. B., Lai W. A., Shen F. T., Rekha P. D., Lin K. Y., Chou J. H..( 2007;). Luteimonas composti sp. nov., a moderately thermophilic bacterium isolated from food waste. . Int J Syst Evol Microbiol 57: 741–744. [CrossRef] [PubMed]
    [Google Scholar]
  37. Zhang D. C., Liu H. C., Xin Y. H., Zhou Y. G., Schinner F., Margesin R..( 2010;). Luteimonas terricola sp. nov., a psychrophilic bacterium isolated from soil. . Int J Syst Evol Microbiol 60: 1581–1584. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001539
Loading
/content/journal/ijsem/10.1099/ijsem.0.001539
Loading

Data & Media loading...

Supplementary File 1

WORD

Supplementary File 2

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error