1887

Abstract

A Gram-stain-negative, rod-shaped bacterium, forming yellow colonies and designated CDR SL 15, was isolated from the surface of sp., a brown macroalga, which grows in the Western coastal regions of the state of Goa, India. The 16S rRNA gene sequence phylogeny placed the strain in the genus and it showed closest sequence similarity to BZ92r (97.6 %) and <97.0 % to other species of the genus . Chemotaxonomic features, such as having iso-C and summed feature 9 (C 10-methyl/iso-Cω9) as the major fatty acids and Q-8 as the only ubiquinone further supported its placement within this genus. There were some critical differences in phenotypic properties between sp. nov. CDR SL 15 and DSM 22344 i.e. temperature range for growth and salinity range and optimum for growth ( is a psychrotolerant bacterium with a lower optimum temperature for growth), acid production and assimilation of substrates, enzyme activities and resistance to certain antibiotics. The DNA–DNA relatedness value of the novel strain with its closest phylogenetic relative was only 40 %, below the 70 % threshold value recommended for species delineation. All these characteristics are consistent with strain CDR SL 15 representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CDR SL 15 (=DSM 101536=KCTC 52403).

Keyword(s): 16S rRNA , CDR-SL15T , FAME , MALDI-TOF and phylogeny
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2016-12-01
2020-03-29
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