1887

Abstract

A bacterial strain designated LYH-20 was isolated from a fish pond in Taiwan and characterized using a polyphasic taxonomy approach. Cells of strain LYH-20 were aerobic, Gram-stain-negative, non-motile, poly-β-hydroxybutyrate containing, showing straight and rod shaped that were covered by large capsules and formed yellow-coloured colonies. Growth occurred at 15–40 °C (optimum, 30 °C), with 0–1.0 % NaCl (optimum, 0–0.1 %) and at pH 5.0–9.0 (optimum, pH 8.0–9.0). According to a phylogenetic tree based on 16S rRNA gene sequence analysis, strain LYH-20 belonged to the genus Sphingomonas and clustered with Sphingomonas fonticola TNR-2, with which it shared the highest 16S rRNA gene sequence similarity (97.5 %). The major fatty acids (>10 %) of strain LYH-20 were C18 : 1ω7c and C16 : 0. The DNA G+C content was 67.5 mol%. The sole isoprenoid quinone was Q-10. The polyamines detected were spermidine, putrescine and homospermidine. The polar lipid profile consisted of a mixture of sphingoglycolipid, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol and three uncharacterized phospholipids. The DNA–DNA hybridization value for strain LYH-20 with Sphingomonas fonticola TNR-2 was less than 35 %. Phenotypic characteristics of the novel strain also differed from those of the closest related species of the genus Sphingomonas . On the basis of the genotypic, chemotaxonomic and phenotypic data, strain LYH-20 represents a novel species in the genus Sphingomonas , for which the name Sphingomonas piscinae sp. nov. is proposed. The type strain is LYH-20 (=BCRC 80911=LMG 29002=KCTC 42741).

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2016-12-01
2019-12-06
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