1887

Abstract

A novel arsenic-resistant bacterium, designated 42-50, was isolated from the high-arsenic sediment of Jianghan Plain, Hubei Province, China. Phylogenetic and biochemical analysis indicated that this bacterium represents the first species of a novel genus belonging to the family . The 16S rRNA gene of strain 42-50 shares 96.3–94.2, 96.3, 96.2 and 94.9–93.8 % sequence identities to those of species from the genera , , and , respectively. The major cellular fatty acids are C, C, C 7 11-methyl and summed feature 8 (comprising C 7c and C 6c). The predominant polar lipids are diphosphatidylglycerol, phosphatidylglycerol and two unidentified glycolipids. The predominant respiratory quinone is ubiquinone-10 (Q-10). The DNA G+C content of strain 42-50 is 73.7 mol%. The distinct phylogenetic lineage and unique cellular fatty acids suggest that strain 42-50 represents a novel species of a new genus affiliated with the family , for which the name gen. nov., sp. nov. is proposed. The type strain is 42-50 (=CCTCC AB 2014325=KCTC 42825).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001543
2016-12-01
2021-10-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/12/5478.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001543&mimeType=html&fmt=ahah

References

  1. Ao L., Zeng X.-C., Nie Y., Mu Y., Zhou L., Luo X. 2014; Flavobacterium arsenatis sp. nov., a novel arsenic-resistant bacterium from high-arsenic sediment. Int J Syst Evol Microbiol 64:3369–3374 [View Article]
    [Google Scholar]
  2. Cao H., Nie Y., Zeng X. C., Xu L., He Z., Luo X., Wu R. 2014; Pontibacter yuliensis sp. nov., isolated from soil. Int J Syst Evol Microbiol 64:968–972 [View Article][PubMed]
    [Google Scholar]
  3. Chen X., Zeng X. C., Wang J., Deng Y., Ma T., Guoji E. 2016; Microbial communities involved in arsenic mobilization and release from the deep sediments into groundwater in Jianghan plain, Central China. Sci Total Environ in press, doi: 10.1016/j.scitotenv.2016.11.024
    [Google Scholar]
  4. Gan Y., Wang Y., Duan Y., Deng Y., Guo X., Ding X. 2014; Hydrogeochemistry and arsenic contamination of groundwater in the Jianghan plain, central China. J Geochem Explor 138:81–93 [View Article]
    [Google Scholar]
  5. Geng S., Pan X.-C., Mei R., Wang Y.-N., Sun J. Q., Liu X.-Y., Tang Y.-Q., Wu X.-L. 2015; Paradevosia shaoguanensis gen. nov., sp. nov., isolated from a coking wastewater. Curr Microbiol 70:110–118 [View Article]
    [Google Scholar]
  6. Huang S. B., Liu C. R., Wang Y. X., Zhan H. B. 2014; Multivariate analysis of the heterogeneous geochemical processes controlling arsenic enrichment in a shallow groundwater system. J Environ Sci Health a Tox Hazard Subst Environ Eng 49:478–489 [View Article][PubMed]
    [Google Scholar]
  7. Jeon C. O., Lim J. M., Lee J. M., Xu L. H., Jiang C. L., Kim C. J. 2005; Reclassification of Bacillus haloalkaliphilus Fritze 1996 as Alkalibacillus haloalkaliphilus gen. nov., comb. nov. and the description of Alkalibacillus salilacus sp. nov., a novel halophilic bacterium isolated from a salt lake in China. Int J Syst Evol Microbiol 55:1891–1896 [View Article][PubMed]
    [Google Scholar]
  8. Komagata K., Suzuki K. 1988; 4 Lipid and cell-wall analysis in bacterial systematics. Method Microbiol 19:161–207 [CrossRef]
    [Google Scholar]
  9. Kumar M., Verma M., Lal R. 2008; Devosia chinhatensis sp. nov., isolated from a hexachlorocyclohexane (HCH) dump site in India. Int J Syst Evol Microbiol 58:861–865 [View Article][PubMed]
    [Google Scholar]
  10. Li Q., Xu Y., Liu K., Cai L., Fu Y., Sun J., Zhang R. 2013; Pelagibacterium nitratireducens sp. nov., a marine Alphaproteobacterium isolated from the East China Sea. Curr Microbiol 66:450–455 [View Article][PubMed]
    [Google Scholar]
  11. Luo X., Wang J., Zeng X.-C., Wang Y., Zhou L., Nie Y., Dai J., Fang C. 2012; Mycetocola manganoxydans sp. nov., an actinobacterium isolated from the Taklamakan desert. Int J Syst Evol Microbiol 62:2967–2970 [View Article]
    [Google Scholar]
  12. Mackie T., McCartney J. E. 1989; Organization of the clinical laboratory. In Mackie and McCartney’s Practical Medical Microbiology, 13th edn. p 21 Edited by Collee J. G., Duguid J. P., Fraser A. G., Marmion B. P. Edinburgh: Churchill Livingstone;
    [Google Scholar]
  13. Mesbah M., Whitman W. B. 1989; Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. J Chromatogr A 479:297–306 [View Article]
    [Google Scholar]
  14. Minnikin D. E., O'donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Meth 2:233–241 [View Article]
    [Google Scholar]
  15. Mu Y., Pan Y., Shi W., Liu L., Jiang Z., Luo X., Zeng X. C., Li W. J. 2016; Luteimonas arsenica sp. nov., an arsenic-tolerant bacterium isolated from arsenic-contaminated soil. Int J Syst Evol Microbiol 66:2291–2296 [View Article][PubMed]
    [Google Scholar]
  16. Nakagawa Y., Sakane T., Yokota A. 1996; Transfer of “Pseudomonas riboflavina” (Foster 1944), a Gram-negative, motile rod with long-chain 3-hydroxy fatty acids, to Devosia riboflavina gen. nov., sp. nov., nom. rev. Int J Syst Bacteriol 46:16–22 [View Article][PubMed]
    [Google Scholar]
  17. Suzuki M., Nakagawa Y., Harayama S., Yamamoto S. 2001; Phylogenetic analysis and taxonomic study of marine Cytophaga-like bacteria: proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb. nov. and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp. nov. and Tenacibaculum amylolyticum sp. nov. Int J Syst Evol Microbiol 51:1639–1652 [View Article][PubMed]
    [Google Scholar]
  18. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013; mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729 [View Article]
    [Google Scholar]
  19. Wang, Huang F. Q., Nogi Y., Pang S. J., Wang P. K., Lv J. 2015; Youhaiella tibetensis gen. nov., sp. nov., isolated from subsurface sediment. Int J Syst Evol Microbiol 65:2048–2055 [View Article][PubMed]
    [Google Scholar]
  20. Xu X.-W., Huo Y.-Y., Wang C.-S., Oren A., Cui H.-L., Vedler E., Wu M. 2011; Pelagibacterium halotolerans gen. nov., sp. nov. and P elagibacterium luteolum sp. nov., novel members of the family Hyphomicrobiaceae . Int J Syst Evol Microbiol 61:1817–1822 [View Article]
    [Google Scholar]
  21. Xu L., Zeng X. C., Nie Y., Luo X., Zhou E., Zhou L., Pan Y., Li W. 2014; Pontibacter diazotrophicus sp. nov., a novel nitrogen-fixing bacterium of the family Cytophagaceae . PLoS One 9:e92294 [View Article][PubMed]
    [Google Scholar]
  22. Zeng X. C., E G., Wang J., Wang N., Chen X., Mu Y., Li H., Yang Y., Liu Y., Wang Y. 2016; Functions and Unique Diversity of Genes and Microorganisms Involved in Arsenite Oxidation from the Tailings of a Realgar Mine. Appl Environ Microbiol 82:7019–7029 [View Article][PubMed]
    [Google Scholar]
  23. Zhu Y. G., Yoshinaga M., Zhao F. J., Rosen B. P. 2014; Earth abides arsenic biotransformations. Annu Rev Earth Planet Sci 42:443–467 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001543
Loading
/content/journal/ijsem/10.1099/ijsem.0.001543
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error