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Abstract

A Gram-stain-negative, aerobic, non-spore-forming, motile and coccoid, ovoid or rod-shaped bacterial strain, designated OITF-36, was isolated from a tidal flat in Oido, an island of South Korea, and subjected to a polyphasic taxonomic study. Strain OITF-36 grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0–3.0 % (w/v) NaCl. Neighbour-joining and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences revealed that strain OITF-36 clustered with the type strains of Primorskyibacter sedentarius and Primorskyibacter insulae , showing 97.4–97.6 % sequence similarity. It exhibited sequence similarity values of less than 96.9 % to the type strains of the other recognized species. Strain OITF-36 contained Q-10 as the predominant ubiquinone and C18 : 1 ω7c as the major fatty acid. The major polar lipids detected in strain OITF-36 were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, one unidentified aminolipid and one unidentified lipid. The DNA G+C content of strain OITF-36 was 61.8 mol%, and its mean DNA–DNA relatedness values with the type strains of P . sedentarius and P . insulae were 16–24 %. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain OITF-36 is separate from P . sedentarius and P . insulae . On the basis of the data presented, strain OITF-36 is considered to represent a novel species of the genus Primorskyibacter , for which the name Primorskyibacter aestuariivivens sp. nov. is proposed. The type strain is OITF-36 (=KCTC 52455=NBRC 112419).

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2016-12-01
2019-10-19
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