1887

Abstract

A novel Gram-stain-negative, small curved-rod-shaped, motile strain, designated L6, was isolated from hydrocarbon-contaminated soils collected from Kuwait. Strain L6 was able to grow at 10–40 °C (optimum, 27–32 °C), pH 6.1–8.8 (optimum, 6.5–7.5) and 0–4.5 % (w/v) NaCl (optimum, 0–0.5). Cω6/Cω7, C, Cω6/Cω7, C and C 3-OH were predominant fatty acids with minor amounts of C and C cyclo. Phosphatidylglycerol and phosphatidylethanolamine were major polar lipids. The genomic G+C content was 61.2 mol%. 16S rRNA gene sequence comparisons indicated that strain L6 represents a member of the genus within the family of the class . Strain L6 has a sequence similarity of 99.2 % with SGLY2 and <93.8 % with other members of the family . However, strain L6 showed only 56.5±2 % relatedness (based on DNA–DNA hybridization) with KACC 12055 (=SGLY2). Distinct morphological, physiological and genotypic differences from the previously described taxa support the classification of strain L6 as a representative of a novel species in the genus , for which the name sp. nov. is proposed. The type strain is L6 (=KEMB 2255-471=JCM 31223). An emended description of the genus is also proposed.

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2016-12-01
2021-10-28
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References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. 1990; Basic local alignment search tool. J Mol Biol 215:403–410 [View Article][PubMed]
    [Google Scholar]
  2. Bushnell L. D., Haas H. F. 1941; The Utilization of certain hydrocarbons by microorganisms. J Bacteriol 41:653–673[PubMed]
    [Google Scholar]
  3. Cappuccino J. G., Sherman N. 1998 Microbiology - a Laboratory Manual, 5th edn. California: Benjamin/Cummings Science Publishing;
    [Google Scholar]
  4. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Evol Microbiol 39:224–229 [View Article]
    [Google Scholar]
  5. Hiraishi A., Hoshino Y., Kitamura H. 1984; Isoprenoid quinone composition in the classification of Rhodospirillaceae. J Gen Appl Microbiol 30:197–210 [View Article]
    [Google Scholar]
  6. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
    [Google Scholar]
  7. Kim H. S., Srinivasan S., Lee S. S. 2016; Methyloterrigena soli gen. nov., sp. nov., a methanol-utilizing bacterium isolated from chloroethylene-contaminated soil. Int J Syst Evol Microbiol 66:101–106 [View Article][PubMed]
    [Google Scholar]
  8. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
    [Google Scholar]
  9. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 3:208–218 [View Article]
    [Google Scholar]
  10. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
    [Google Scholar]
  11. Oren A., Duker S., Ritter S. 1996; The polar lipid composition of walsby's square bacterium. FEMS Microbiol Lett 138:135–140 [View Article]
    [Google Scholar]
  12. Patureau D., Godon J. J., Dabert P., Bouchez T., Bernet N., Delgenes J. P., Moletta R. 1998; Microvirgula aerodenitrificans gen. nov., sp. nov., a new gram-negative bacterium exhibiting co-respiration of oxygen and nitrogen oxides up to oxygen-saturated conditions. Int J Syst Bacteriol 48:775–782 [View Article][PubMed]
    [Google Scholar]
  13. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  14. Subhash Y., Sasikala C., Ramana C. 2013a; Flavobacterium aquaticum sp. nov., isolated from a water sample of a rice field. Int J Syst Evol Microbiol 63:3463–3469 [View Article][PubMed]
    [Google Scholar]
  15. Subhash Y., Tushar L., Sasikala C., Ramana C. 2013b; Erythrobacter odishensis sp. nov. and Pontibacter odishensis sp. nov. isolated from dry soil of a solar saltern. Int J Syst Evol Microbiol 63:4524–4532 [View Article][PubMed]
    [Google Scholar]
  16. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013; mega6: molecular evolutionary genetics analysis version 6.0. Mol Bio Evol 30:2725–2729 [View Article]
    [Google Scholar]
  17. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. 1987; Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Evol Microbiol 37:463–464 [View Article]
    [Google Scholar]
  18. Xu M., Xin Y., Yu Y., Zhang J., Zhou Y., Liu H., Tian J., Li Y. 2010; Erythrobacter nanhaisediminis sp. nov., isolated from marine sediment of the South China Sea. Int J Syst Evol Microbiol 60:2215–2220 [View Article][PubMed]
    [Google Scholar]
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