1887

Abstract

A novel Gram-stain-negative, small curved-rod-shaped, motile strain, designated L6, was isolated from hydrocarbon-contaminated soils collected from Kuwait. Strain L6 was able to grow at 10–40 °C (optimum, 27–32 °C), pH 6.1–8.8 (optimum, 6.5–7.5) and 0–4.5 % (w/v) NaCl (optimum, 0–0.5). C18 : 1ω6c/C18 : 1ω7c, C16 : 0, C16 : 1ω6c/C16 : 1ω7c, C12 : 0 and C12 : 0 3-OH were predominant fatty acids with minor amounts of C14 : 0 and C17 : 0 cyclo. Phosphatidylglycerol and phosphatidylethanolamine were major polar lipids. The genomic G+C content was 61.2 mol%. 16S rRNA gene sequence comparisons indicated that strain L6 represents a member of the genus Microvirgula within the family Neisseriaceae of the class Betaproteobacteria . Strain L6 has a sequence similarity of 99.2 % with Microvirgula aerodenitrificans SGLY2 and <93.8 % with other members of the family Neisseriaceae . However, strain L6 showed only 56.5±2 % relatedness (based on DNA–DNA hybridization) with M. aerodenitrificans KACC 12055 (=SGLY2). Distinct morphological, physiological and genotypic differences from the previously described taxa support the classification of strain L6 as a representative of a novel species in the genus Microvirgula , for which the name Microvirgula curvata sp. nov. is proposed. The type strain is L6 (=KEMB 2255-471=JCM 31223). An emended description of the genus Microvirgula is also proposed.

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2016-12-01
2019-10-15
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