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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo - Volume 65, Issue Pt_10, 2015

Volume 65, Issue Pt_10

Volume 65, Issue Pt_10, 2015

  • NEW TAXA
    • Eukaryotic micro-organisms

      • sp. nov., a yeast species consistently isolated from tree bark in the Canadian province of Québec

        https://doi.org/10.1099/ijsem.0.000426

        A thorough sampling of maple, oak, birch, and apple tree bark in North America yielded a set of isolates that represent a yeast species not yet formally described. The strains obtained were all isolated from the Canadian province of Québec. These four isolates have identical electrophoretic karyotypes, distinct from other species of the genus , and are most closely related to the formally recognized species according to the D1/D2 domain of the LSU rDNA gene and 5.8S–ITS region. Previous studies revealed the existence of a population of strains closely related to with unique D1/D2 sequences and the ability to grow on melibiose, which is also true for these isolates. The sequences obtained here (for the D1/D2, and 5.8S–ITS region) are identical among the four strains, and in a phylogenetic analysis of the D1/D2 region, the strains form a distinct clade with the previously described population closely related to , composed of isolates from Japan, as well as from the provinces of Ontario and Québec in Canada. On the basis of select physiological and phylogenetic characteristics, a novel ascosporogenous yeast species, sp. nov., is proposed. The type strain LL11_022 ( = CBS 14138 = CLIB 1763 = UCDFST 15-106) was isolated from maple tree bark in the Station Duchesnay, QC region of Québec, Canada. The MycoBank number is MB811749.

    • sp. nov., a yeast isolated from Bandji, a traditional palm wine of

      https://doi.org/10.1099/ijsem.0.000461

      Investigation of the microbial diversity of Bandji, a traditional palm wine from Burkina Faso (West Africa) revealed the presence of two yeast isolates (YAV16 and YAV17) with unusual phenotypic and genotypic characteristics. The isolates divide by bipolar budding with no production of ascospores. Phylogenetic analysis of concatenated sequences of the 26S rRNA gene D1/D2 and internal transcribed spacer (ITS) regions indicated that the novel species was most closely related to and . The new isolates differed from NRRL Y-17531 and CBS 479 by substitutions in the D1/D2 region of 12 and 16 nt respectively. The divergence in the ITS region from the closely related species was characterized by substitutions of 45–46 nt. Repetitive palindromic PCR (rep-PCR) profiles of YAV16 and YAV17 were also significantly different from those of MUCL 31146 ( = NRRL Y-17531), NCYC 17 ( = CBS 479) and other species of the genus . Based on the results of the phenotypic and genotypic characterizations, it was concluded that the new isolates represent a novel species for which the name sp. nov. is proposed with YAV17 ( = CBS 12942 = DSM 26339 = NCYC 3828; MycoBank number MB 805785) as the type strain.

    • sp. nov. and sp. nov., two ascosporic yeast species isolated from the gut of beetles

      https://doi.org/10.1099/ijsem.0.000460

      Four yeast strains were isolated from the gut of beetles collected on Baotianman Mountain and People's Park of Nanyang in Henan Province, China. These strains produced unconjugated asci with one or two ellipsoidal to elongate ascospores in a persistent ascus. Phylogenetic analysis of the D1/D2 domains of the LSU rRNA gene sequences indicated that the isolates represent two novel sexual species in the / clade. sp. nov. was located in a statistically well-supported branch together with . sp. nov. formed a subclade with its closest relative supported by a strong bootstrap value. The two novel species were distinguished from their most closely related described species, and , in the D1/D2 LSU rRNA gene and internal transcribed spacer (ITS) sequences and in phenotypic traits. The type strain of sp. nov. is NYNU 14719 ( = CBS 13915 = CICC 33052), and the type strain of sp. nov. is NYNU 14772 ( = CBS 13916 = CICC 33053). The MycoBank numbers for sp. nov. and sp. nov. are MB 812621 and MB 812622.

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