1887

Abstract

Investigation of the microbial diversity of Bandji, a traditional palm wine from Burkina Faso (West Africa) revealed the presence of two yeast isolates (YAV16 and YAV17) with unusual phenotypic and genotypic characteristics. The isolates divide by bipolar budding with no production of ascospores. Phylogenetic analysis of concatenated sequences of the 26S rRNA gene D1/D2 and internal transcribed spacer (ITS) regions indicated that the novel species was most closely related to and . The new isolates differed from NRRL Y-17531 and CBS 479 by substitutions in the D1/D2 region of 12 and 16 nt respectively. The divergence in the ITS region from the closely related species was characterized by substitutions of 45–46 nt. Repetitive palindromic PCR (rep-PCR) profiles of YAV16 and YAV17 were also significantly different from those of MUCL 31146 ( = NRRL Y-17531), NCYC 17 ( = CBS 479) and other species of the genus . Based on the results of the phenotypic and genotypic characterizations, it was concluded that the new isolates represent a novel species for which the name sp. nov. is proposed with YAV17 ( = CBS 12942 = DSM 26339 = NCYC 3828; MycoBank number MB 805785) as the type strain.

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2015-10-01
2019-12-06
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