- Volume 33, Issue 2, 1983

Genotypic and phenotypic data (results of deoxyribonucleic acid-ribosomal ribonucleic acid and deoxyribonucleic acid-deoxyribonucleic acid hybridizations, guanine-plus-cytosine content, comparative enzymology, type of ubiquinones, cellular fatty acid composition, growth, niches, and several other characteristics) showed that Pseudomonas maltophilia (Hugh 1981) is generically misnamed and should be transferred to Xanthomonas as Xanthomonas maltophilia (Hugh 1981) comb. nov.

We propose guidelines for terminology for the immunodiffusion and immunoelectrophoresis methods used in serotaxonomy. These guidelines were prepared at the request of the International Working Group on Mycobacterial Taxonomy, which endorsed them in 1981.

The proteins of nine strains of Ureaplasma urealyticum, representing serovars I through IX, were analyzed by using two-dimensional gel electrophoresis and a sensitive silver staining procedure. The electrophoretic patterns of the nine strains tested could be divided into two distinct major groups, one group consisting of serovars II, IV, V, VII, VIII, and IX (group A) and one group consisting of serovars I, III, and VI (group B).

The relationship among 22 strains of Actinobacillus actinomycetemcomitans, 3 strains of Haemophilus aphrophilus, 1 reference strain of Actinobacillus lignieresii, and the type strain of Actinobacillus equuli were assessed by deoxyribonucleic acid (DNA)-DNA hybridizations on membrane filters. We found that the A. actinomycetemcomitans strains formed a homogeneous group that was distinct from H. aphrophilus and the other Actinobacillus species. There was about 40% hybridization between H. aphrophilus DNA and A. actinomycetemcomitans DNA, indicating some similarity, but A. lignieresii and A. equuli DNAs hybridized much less (never more than 20%) with A. actinomycetemcomitans DNA.

Four species were listed in the Approved Lists of Bacterial Names with hyphenated specific epithets, which is in conflict with Rule 12a of the International Code of Nomenclature of Bacteria. The hyphens are dropped, and the names are corrected to Nocardia otitidiscaviarum, Propionibacterium acidipropionici, Salmonella choleraesuis, and Treponema paraluiscuniculi.

The type strain of Lactobacillus crispatus (VPI 3199, parent strain of strain ATCC 33820) and the deoxyribonucleic acid homology reference strain of “Lactobacillus acidophilus” group A2 (strain ATCC 33197) have 100% deoxyribonucleic acid homology. L. crispatus and strains in “L. acidophilus” group A2 also produce similar polyacrylamide gel electrophoresis patterns of soluble cellular proteins and are similar in other phenotypic characteristics. Therefore, the group A2 strains are members of the species L. crispatus (Brygoo and Aladame 1953) Moore and Holdeman 1970.

We propose that the Judicial Commission of the International Committee on Systematic Bacteriology conserve the genus name Micropolyspora with Micropolyspora faeni Cross, Maciver, and Lacey 1968 (Approved Lists of Bacterial Names, 1980) as the type species. This proposal is made in the interest of nomenclatural stability and in order to avoid confusion in the literature after the reclassification of the original type species, Micropolyspora brevicatena Lechevalier, Solotorovsky, and McDurmont 1969 (Approved Lists, 1980), as Nocardia brevicatena (Lechevalier, Solotorovosky, and McDurmont) Goodfellow and Pirouz 1982 and demonstration of the synonymy of M. faeni with Micropolyspora rectivirgula Krassilnikov and Agre 1964 (Approved Lists, 1980). M. faeni is the main causative agent of farmer&s lung disease and other forms of hypersensitivity pneumonitis. Consequently, the binomial M. faeni has been used in numerous scientific and medical publications, whereas M. rectivirgula appears in few. The genus Micropolyspora so conserved would also include Micropolyspora angiospora Zhukova, Tsyganov, and Morozov 1968 (Approved Lists, 1980) and Micropolyspora internatus Agre, Guzeva, and Dorkhova 1974 (Approved Lists, 1980).

The Judicial Commission designates Eubacterium limosum (Eggerth) Prévot 1938 as the type species of Eubacterium Prévot 1938. The type strain is ATCC 8486.