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The relationship among 22 strains of Actinobacillus actinomycetemcomitans, 3 strains of Haemophilus aphrophilus, 1 reference strain of Actinobacillus lignieresii, and the type strain of Actinobacillus equuli were assessed by deoxyribonucleic acid (DNA)-DNA hybridizations on membrane filters. We found that the A. actinomycetemcomitans strains formed a homogeneous group that was distinct from H. aphrophilus and the other Actinobacillus species. There was about 40% hybridization between H. aphrophilus DNA and A. actinomycetemcomitans DNA, indicating some similarity, but A. lignieresii and A. equuli DNAs hybridized much less (never more than 20%) with A. actinomycetemcomitans DNA.
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