1887

Abstract

One novel actinobacterial isolate, designated strain NEAU-G17, was isolated from muddy soil collected from a riverbank in Chad and characterised using polyphasic approach. Morphological and chemotaxonomic characteristics of this strain coincided with those of the genus . The 16S rRNA gene sequence analysis showed that strain NEAU-G17 was most closely related to JCM 10648 (98.7 %) and NEAU-Gz5 (98.6 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NEAU-G17 formed a cluster with JCM 10648, NEAU-Gz5 and CS5-AC15 (96.4 %). However, the combination of DNA–DNA hybridisation values and some phenotypic characteristics allowed the isolate to be differentiated from their most closely related species. The major menaquinones were identified as MK-9(H), MK-9(H) and MK-9(H). Their polar lipid profile was found to contain diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside, an unidentified phospholipid and an unidentified lipid. The major fatty acids were identified as C, -C and C ω9c. Therefore, it is proposed that strain NEAU-G17 should be classified as representative of a novel species of the genus , for which the name sp. nov. is proposed. The type strains is NEAU-G17 (=CGMCC 4.7411=DSM 105122).

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2019-09-01
2019-10-21
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