%0 Journal Article %A Shi, Linlin %A Han, Liyuan %A Guo, Xiaowei %A Zhao, Junwei %A Wang, Jiabin %A Wang, Han %A Jiang, Shanwen %A Xiang, Wensheng %A Wang, Xiangjing %T Actinomadura logoneensis sp. nov., a novel actinomycete isolated from the soil %D 2019 %J International Journal of Systematic and Evolutionary Microbiology, %V 69 %N 9 %P 2914-2920 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.003579 %K Actinomadura logoneensis sp. nov. %K 16S rRNA gene %K Polyphasic analysis %I Microbiology Society, %X One novel actinobacterial isolate, designated strain NEAU-G17T, was isolated from muddy soil collected from a riverbank in Chad and characterised using polyphasic approach. Morphological and chemotaxonomic characteristics of this strain coincided with those of the genus Actinomadura . The 16S rRNA gene sequence analysis showed that strain NEAU-G17T was most closely related to Actinomadura oligospora JCM 10648T (98.7 %) and Actinomadura gamaensis NEAU-Gz5T (98.6 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NEAU-G17T formed a cluster with Actinomadura oligospora JCM 10648T, Actinomadura gamaensis NEAU-Gz5T and Actinomadura rupiterrae CS5-AC15T (96.4 %). However, the combination of DNA–DNA hybridisation values and some phenotypic characteristics allowed the isolate to be differentiated from their most closely related species. The major menaquinones were identified as MK-9(H4), MK-9(H6) and MK-9(H8). Their polar lipid profile was found to contain diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside, an unidentified phospholipid and an unidentified lipid. The major fatty acids were identified as C16 : 0, iso-C16 : 0 and C18 : 1 ω9c. Therefore, it is proposed that strain NEAU-G17T should be classified as representative of a novel species of the genus Actinomadura , for which the name Actinomadura logoneensis sp. nov. is proposed. The type strains is NEAU-G17T (=CGMCC 4.7411T=DSM 105122T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003579