@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.003579, author = "Shi, Linlin and Han, Liyuan and Guo, Xiaowei and Zhao, Junwei and Wang, Jiabin and Wang, Han and Jiang, Shanwen and Xiang, Wensheng and Wang, Xiangjing", title = "Actinomadura logoneensis sp. nov., a novel actinomycete isolated from the soil", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2019", volume = "69", number = "9", pages = "2914-2920", doi = "https://doi.org/10.1099/ijsem.0.003579", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003579", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Actinomadura logoneensis sp. nov.", keywords = "16S rRNA gene", keywords = "Polyphasic analysis", abstract = "One novel actinobacterial isolate, designated strain NEAU-G17T, was isolated from muddy soil collected from a riverbank in Chad and characterised using polyphasic approach. Morphological and chemotaxonomic characteristics of this strain coincided with those of the genus Actinomadura . The 16S rRNA gene sequence analysis showed that strain NEAU-G17T was most closely related to Actinomadura oligospora JCM 10648T (98.7 %) and Actinomadura gamaensis NEAU-Gz5T (98.6 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NEAU-G17T formed a cluster with Actinomadura oligospora JCM 10648T, Actinomadura gamaensis NEAU-Gz5T and Actinomadura rupiterrae CS5-AC15T (96.4 %). However, the combination of DNA–DNA hybridisation values and some phenotypic characteristics allowed the isolate to be differentiated from their most closely related species. The major menaquinones were identified as MK-9(H4), MK-9(H6) and MK-9(H8). Their polar lipid profile was found to contain diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside, an unidentified phospholipid and an unidentified lipid. The major fatty acids were identified as C16 : 0, iso-C16 : 0 and C18 : 1 ω9c. Therefore, it is proposed that strain NEAU-G17T should be classified as representative of a novel species of the genus Actinomadura , for which the name Actinomadura logoneensis sp. nov. is proposed. The type strains is NEAU-G17T (=CGMCC 4.7411T=DSM 105122T).", }