1887

Abstract

A Gram-positive, moderately halophilic, motile, strictly aerobic, endospore-forming, oxidase- and catalase-positive, rod-shaped bacterium, strain YIM kkny16, was isolated from a saline mud sample collected from the Keke salt lake in the Qaidam Basin, north-west China. This isolate grew in the presence of 0–25 % (w/v) NaCl and at pH 6.0–10.0 and 10–50 °C; optimum growth was observed with 10 % (w/v) NaCl and at pH 7.0 and 37 °C. Strain YIM kkny16 had -diaminopimelic acid as the diagnostic diamino acid, MK-7 as the predominant respiratory quinone, with a significant amount of MK-6, and anteiso-C, iso-C and C 7 alcohol as major fatty acids. Major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The DNA G+C content was 41.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences confirmed that strain YIM kkny16 was a member of the genus , exhibiting sequence similarities of 94.9–97.3 % to the type strains of recognized species. Strain YIM kkny16 could be differentiated from recognized species based on phenotypic characteristics, chemotaxonomic differences, phylogenetic analysis and DNA–DNA hybridization data. On the basis of evidence from this polyphasic study, strain YIM kkny16 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YIM kkny16 (=DSM 17056=CGMCC 1.6298).

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2008-03-01
2020-01-23
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