%0 Journal Article %A Chen, Yi-Guang %A Cui, Xiao-Long %A Fritze, Dagmar %A Chai, Li-Hong %A Schumann, Peter %A Wen, Meng-Liang %A Wang, Yong-Xia %A Xu, Li-Hua %A Jiang, Cheng-Lin %T Virgibacillus kekensis sp. nov., a moderately halophilic bacterium isolated from a salt lake in China %D 2008 %J International Journal of Systematic and Evolutionary Microbiology, %V 58 %N 3 %P 647-653 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.65365-0 %I Microbiology Society, %X A Gram-positive, moderately halophilic, motile, strictly aerobic, endospore-forming, oxidase- and catalase-positive, rod-shaped bacterium, strain YIM kkny16T, was isolated from a saline mud sample collected from the Keke salt lake in the Qaidam Basin, north-west China. This isolate grew in the presence of 0–25 % (w/v) NaCl and at pH 6.0–10.0 and 10–50 °C; optimum growth was observed with 10 % (w/v) NaCl and at pH 7.0 and 37 °C. Strain YIM kkny16T had meso-diaminopimelic acid as the diagnostic diamino acid, MK-7 as the predominant respiratory quinone, with a significant amount of MK-6, and anteiso-C15 : 0, iso-C14 : 0 and C16 : 1 ω7c alcohol as major fatty acids. Major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The DNA G+C content was 41.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences confirmed that strain YIM kkny16T was a member of the genus Virgibacillus, exhibiting sequence similarities of 94.9–97.3 % to the type strains of recognized Virgibacillus species. Strain YIM kkny16T could be differentiated from recognized Virgibacillus species based on phenotypic characteristics, chemotaxonomic differences, phylogenetic analysis and DNA–DNA hybridization data. On the basis of evidence from this polyphasic study, strain YIM kkny16T is considered to represent a novel species of the genus Virgibacillus, for which the name Virgibacillus kekensis sp. nov. is proposed. The type strain is YIM kkny16T (=DSM 17056T=CGMCC 1.6298T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.65365-0