1887

Abstract

A facultatively anaerobic, moderately alkaliphilic, Gram-positive, spore-forming and rod-shaped bacterial strain, Shu-P-Ggiii25-2, was isolated from lake sediment in Shizuoka, Japan, and was characterized taxonomically using a polyphasic approach. Analysis of the 16S rRNA gene sequence indicated that the novel isolate clustered with the type strain of and it exhibited sequence similarities of 94.9–95.6 % to the type strains of species of the genus . Isoprenoid quinones and oxidase and catalase activities were not detected for strain Shu-P-Ggiii25-2. The DNA G+C content was 42.3 mol%, the cell-wall peptidoglycan contained -diaminopimelic acid as the diagnostic diamino acid and the major cellular fatty acids were iso-C, iso-C, anteiso-C, C and iso-C. On the basis of phylogenetic position and phenotypic properties, strain Shu-P-Ggiii25-2 represents a novel species of the genus for which the name sp. nov. is proposed. The type strain is Shu-P-Ggiii25-2 (=MBIC08269=IAM 15428=KCTC 13120). An emended description of the genus is also given.

Keyword(s): BTB, bromothymol blue
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64926-0
2007-11-01
2020-09-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/11/2489.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64926-0&mimeType=html&fmt=ahah

References

  1. Collins M. D., Jones D. 1981; Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implications. Microbiol Rev 45:316–354
    [Google Scholar]
  2. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  3. Goto K., Omura T., Hara Y., Sadaie Y. 2000; Application of the partial 16S rDNA sequence as an index for rapid identification of species in the genus Bacillus . J Gen Appl Microbiol 46:1–8 [CrossRef]
    [Google Scholar]
  4. Goto K., Mochida K. M., Asahara M., Suzuki M., Yokota A. 2002; Application of the hypervariable region of the 16S rDNA sequence as an index for the rapid identification of species in the genus Alicyclobacillus . J Gen Appl Microbiol 48:243–250 [CrossRef]
    [Google Scholar]
  5. Ishikawa M., Ishizaki S., Yamamoto Y., Yamasato K. 2002; Paraliobacillus ryukyuensis gen. nov., sp. nov., a new Gram-positive, slightly halophilic, extremely halotolerant, facultative anaerobe isolated from a decomposing marine alga. J Gen Appl Microbiol 48:269–279 [CrossRef]
    [Google Scholar]
  6. Ishikawa M., Nakajima K., Itamiya Y., Furukawa S., Yamamoto Y., Yamasato K. 2005; Halolactibacillus halophilus gen. nov., sp. nov. and Halolactibacillus miurensis sp. nov.,halophilic and alkaliphilic marine lactic acid bacteria constituting a phylogenetic lineage in Bacillus rRNA group 1. Int J Syst Evol Microbiol 55:2427–2439 [CrossRef]
    [Google Scholar]
  7. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  8. Kumar S., Tamura K., Jakobsen I. B., Nei M. 2004; mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [CrossRef]
    [Google Scholar]
  9. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  10. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  11. Niimura Y., Koh E., Yanagida F., Suzuki K., Komagata K., Kozaki M. 1990; Amphibacillus xylanus gen. nov., sp. nov., a facultatively anaerobic sporeforming xylan-digesting bacterium which lacks cytochrome, quinone, and catalase.. Int J Syst Bacteriol 40:297–301 [CrossRef]
    [Google Scholar]
  12. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  13. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477
    [Google Scholar]
  14. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  15. Zhilina T. N., Garnova E. S., Tourova T. P., Kostrikina N. A., Zavarzin G. A. 2001; Amphibacillus fermentum sp. nov. and Amphibacillus tropicus sp. nov., new alkaliphilic, facultatively anaerobic, saccharolytic bacilli from Lake Magadi. Microbiology (English translation of Mikrobiologiia ) 70:711–722
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64926-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64926-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error