%0 Journal Article %A An, Sun-Young %A Ishikawa, Shu %A Kasai, Hiroaki %A Goto, Keiichi %A Yokota, Akira %T Amphibacillus sediminis sp. nov., an endospore-forming bacterium isolated from lake sediment in Japan %D 2007 %J International Journal of Systematic and Evolutionary Microbiology, %V 57 %N 11 %P 2489-2492 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.64926-0 %K BTB, bromothymol blue %I Microbiology Society, %X A facultatively anaerobic, moderately alkaliphilic, Gram-positive, spore-forming and rod-shaped bacterial strain, Shu-P-Ggiii25-2T, was isolated from lake sediment in Shizuoka, Japan, and was characterized taxonomically using a polyphasic approach. Analysis of the 16S rRNA gene sequence indicated that the novel isolate clustered with the type strain of Amphibacillus xylanus and it exhibited sequence similarities of 94.9–95.6 % to the type strains of species of the genus Amphibacillus. Isoprenoid quinones and oxidase and catalase activities were not detected for strain Shu-P-Ggiii25-2T. The DNA G+C content was 42.3 mol%, the cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid and the major cellular fatty acids were iso-C14 : 0, iso-C15 : 0, anteiso-C15 : 0, C16 : 0 and iso-C16 : 0. On the basis of phylogenetic position and phenotypic properties, strain Shu-P-Ggiii25-2T represents a novel species of the genus Amphibacillus, for which the name Amphibacillus sediminis sp. nov. is proposed. The type strain is Shu-P-Ggiii25-2T (=MBIC08269T=IAM 15428T=KCTC 13120T). An emended description of the genus Amphibacillus is also given. %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.64926-0