1887

Abstract

A bacterial isolate, designated strain TS3, was isolated from soil collected from a metal mine in Tieshan District, Daye City, Hubei Province, in central China. Cells of this strain were Gram-negative, motile and rod-shaped. The strain had ubiquinone Q-8 as the predominant respiratory quinone, phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol as the major polar lipids and summed feature 3 (Cω7 and/or iso-C 2-OH), C and Cω7 as the major fatty acids. The G+C content was 65.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain TS3 was most closely related to 5516S-1 (98.5 %), CCUG 58010 (97.6 %), 5516S-11 (97.4 %) and CCUG 35299 (97.2 %). DNA–DNA hybridization revealed low relatedness between strain TS3 and KACC 12599 (36.5 %), CCUG 58010 (27.1 %), KACC 12505 (22.7 %) and CCUG 35299 (46.5 %). On the basis of phenotypic and phylogenetic characteristics, strain TS3 belongs to the genus , but is clearly differentiated from other members of the genus. The strain represents a novel species, for which the name sp. nov. is proposed. The type strain is TS3 ( = CCTCC AB 2010202  = KACC 14940).

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2012-10-01
2019-12-06
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