RT Journal Article SR Electronic(1) A1 Du, Yan A1 Yu, Xiang A1 Wang, GejiaoYR 2012 T1 Massilia tieshanensis sp. nov., isolated from mining soil JF International Journal of Systematic and Evolutionary Microbiology, VO 62 IS Pt_10 SP 2356 OP 2362 DO https://doi.org/10.1099/ijs.0.034306-0 PB Microbiology Society, SN 1466-5034, AB A bacterial isolate, designated strain TS3T, was isolated from soil collected from a metal mine in Tieshan District, Daye City, Hubei Province, in central China. Cells of this strain were Gram-negative, motile and rod-shaped. The strain had ubiquinone Q-8 as the predominant respiratory quinone, phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol as the major polar lipids and summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH), C16 : 0 and C18 : 1ω7c as the major fatty acids. The G+C content was 65.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain TS3T was most closely related to Massilia niastensis 5516S-1T (98.5 %), Massilia consociata CCUG 58010T (97.6 %), Massilia aerilata 5516S-11T (97.4 %) and Massilia varians CCUG 35299T (97.2 %). DNA–DNA hybridization revealed low relatedness between strain TS3T and M. niastensis KACC 12599T (36.5 %), M. consociata CCUG 58010T (27.1 %), M. aerilata KACC 12505T (22.7 %) and M. varians CCUG 35299T (46.5 %). On the basis of phenotypic and phylogenetic characteristics, strain TS3T belongs to the genus Massilia , but is clearly differentiated from other members of the genus. The strain represents a novel species, for which the name Massilia tieshanensis sp. nov. is proposed. The type strain is TS3T ( = CCTCC AB 2010202T  = KACC 14940T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.034306-0