1887

Abstract

A Gram-negative, rod-shaped bacterium, designated strain YC6274, was isolated from a stream (Sasang) carrying wastewater polluted with heavy metals in Busan, Korea. Growth was observed at 10–35 °C (optimum, 30 °C) and pH 6.0–9.5 (optimum, pH 7.5–8.0). Comparative 16S rRNA gene sequence analyses showed that the strain was most closely related to R2A45-3 (96.6 % similarity), ATCC 11947 (93.7 %), EMB47 (93.3 %), GPTSA100-9 (93.3 %) and DS-20 (93.2 %). Sequence similarities with strains of other species with validly published names were lower than 93.0 %. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain YC6274 formed a distinct phyletic lineage within the genus . The predominant fatty acids of strain YC6274 were iso-C, iso-C, iso-C G, iso-C 3-OH, iso-C 3-OH and iso-C 3-OH. The G+C content of the genomic DNA was 32.5 mol% and the major quinone was MK-6. On the basis of phenotypic, chemotaxonomic and molecular data, it is clear that strain YC6274 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is YC6274 (=KCTC 22246 =DSM 21067).

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2009-05-01
2020-01-23
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Transmission electron micrograph of a cell of strain YC6274 from a 2-day culture cultivated at 30 °C in R2A broth. Bar, 0.5 µm.

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Maximum-likelihood and maximum-parsimony trees based on 16S rRNA gene sequences showing phylogenetic relationships of strain YC6274 and related taxa. [PDF](20 KB)

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