%0 Journal Article %A Yoon, Hwan Sik %A Aslam, Zubair %A Song, Geun Cheol %A Kim, Seon Won %A Jeon, Che Ok %A Chon, Tae Soo %A Chung, Young Ryun %T Flavobacterium sasangense sp. nov., isolated from a wastewater stream polluted with heavy metals %D 2009 %J International Journal of Systematic and Evolutionary Microbiology, %V 59 %N 5 %P 1162-1166 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.004978-0 %K NJ, neighbour-joining %K MP, maximum-parsimony %K ML, maximum-likelihood %I Microbiology Society, %X A Gram-negative, rod-shaped bacterium, designated strain YC6274T, was isolated from a stream (Sasang) carrying wastewater polluted with heavy metals in Busan, Korea. Growth was observed at 10–35 °C (optimum, 30 °C) and pH 6.0–9.5 (optimum, pH 7.5–8.0). Comparative 16S rRNA gene sequence analyses showed that the strain was most closely related to Flavobacterium cucumis R2A45-3T (96.6 % similarity), F. aquatile ATCC 11947T (93.7 %), F. croceum EMB47T (93.3 %), F. indicum GPTSA100-9T (93.3 %) and F. terrigena DS-20T (93.2 %). Sequence similarities with strains of other Flavobacterium species with validly published names were lower than 93.0 %. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain YC6274T formed a distinct phyletic lineage within the genus Flavobacterium. The predominant fatty acids of strain YC6274T were iso-C15 : 0, iso-C16 : 0, iso-C15 : 1 G, iso-C17 : 0 3-OH, iso-C16 : 0 3-OH and iso-C15 : 0 3-OH. The G+C content of the genomic DNA was 32.5 mol% and the major quinone was MK-6. On the basis of phenotypic, chemotaxonomic and molecular data, it is clear that strain YC6274T represents a novel species within the genus Flavobacterium, for which the name Flavobacterium sasangense sp. nov. is proposed. The type strain is YC6274T (=KCTC 22246T =DSM 21067T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.004978-0