@article{mbs:/content/journal/ijsem/10.1099/ijs.0.004978-0, author = "Yoon, Hwan Sik and Aslam, Zubair and Song, Geun Cheol and Kim, Seon Won and Jeon, Che Ok and Chon, Tae Soo and Chung, Young Ryun", title = "Flavobacterium sasangense sp. nov., isolated from a wastewater stream polluted with heavy metals", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2009", volume = "59", number = "5", pages = "1162-1166", doi = "https://doi.org/10.1099/ijs.0.004978-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.004978-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "NJ, neighbour-joining", keywords = "MP, maximum-parsimony", keywords = "ML, maximum-likelihood", abstract = "A Gram-negative, rod-shaped bacterium, designated strain YC6274T, was isolated from a stream (Sasang) carrying wastewater polluted with heavy metals in Busan, Korea. Growth was observed at 10–35 °C (optimum, 30 °C) and pH 6.0–9.5 (optimum, pH 7.5–8.0). Comparative 16S rRNA gene sequence analyses showed that the strain was most closely related to Flavobacterium cucumis R2A45-3T (96.6 % similarity), F. aquatile ATCC 11947T (93.7 %), F. croceum EMB47T (93.3 %), F. indicum GPTSA100-9T (93.3 %) and F. terrigena DS-20T (93.2 %). Sequence similarities with strains of other Flavobacterium species with validly published names were lower than 93.0 %. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain YC6274T formed a distinct phyletic lineage within the genus Flavobacterium. The predominant fatty acids of strain YC6274T were iso-C15 : 0, iso-C16 : 0, iso-C15 : 1 G, iso-C17 : 0 3-OH, iso-C16 : 0 3-OH and iso-C15 : 0 3-OH. The G+C content of the genomic DNA was 32.5 mol% and the major quinone was MK-6. On the basis of phenotypic, chemotaxonomic and molecular data, it is clear that strain YC6274T represents a novel species within the genus Flavobacterium, for which the name Flavobacterium sasangense sp. nov. is proposed. The type strain is YC6274T (=KCTC 22246T =DSM 21067T).", }