1887

Abstract

The similarities of various strains of spp. and spp. to reference strains of , and were determined by rocket line immunoelectrophoresis. The strains of and used were immunologically more homogeneous than the strains of studied, possibly due to the more diverse geographical origins of the strains. Low values were obtained for the mean immunological distances (1 – proportion of immunoprecipitation bands shared between strains) between and strains, suggesting that these two species are immunologically closely related. Immunological distances from the reference strain were similar for and for other undisputed members of the genus , which makes it reasonable to retain in this genus. When the three antisera were used, all species had mean immunological distances of less than 0.5, whereas the species were immunologically more distant, showing that the six species of form an immunologically related group which is distinct from the species. Our results with the three antisera show that each species of is immunologically distant from the other species, as well as from the species. We compare our immunoelectrophoretic results with the molecular biological results of De Smedt et al. (Int. J. Syst. Bacteriol. :106–122, 1980) and the numerical taxonomic analysis of Thompson and Skerman (, Academic Press, Inc., London, 1979).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-33-2-147
1983-04-01
2022-07-01
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/33/2/ijs-33-2-147.html?itemId=/content/journal/ijsem/10.1099/00207713-33-2-147&mimeType=html&fmt=ahah

References

  1. Baillie A., Hodgkiss W., Norris J.R. 1962; Flagellation of Azotobacter spp. as demonstrated by electron microscopy. J. Appl. Bacteriol 25:116–119
    [Google Scholar]
  2. De Ley J., Segers P., Gillis M. 1978; Intra-and intergeneric similarities of Chromobacterium and Janthinobacterium ribosomal ribonucleic acid cistrons. Int. J. Syst. Bacteriol 28:154–168
    [Google Scholar]
  3. De Smedt J., Bauwens M., Tytgat R., De Ley J. 1980; Intra-and intergeneric similarities of ribosomal ribonucleic acid cistrons of free-living, nitrogen-fixing bacteria. Int. J. Syst. Bacteriol 30:106–122
    [Google Scholar]
  4. De Smedt J., De Ley J. 1977; Intra-and intergeneric similarities of Agrobacterium ribosomal ribonucleic acid cistrons. Int. J. Syst. Bacteriol 27:222–240
    [Google Scholar]
  5. Dobereiner J. 1966; Azotobacter paspali sp. n., uma bacteria fixadora de nitrogenia na rizosfera de Paspalum. Pesqui. Agropecu. Bras 1:357–365
    [Google Scholar]
  6. Dubnau D., Smith I., Morrell P., Mannur J. 1965; Gene conservation in Bacillus species. I. Conserved generic and nucleic acid base sequence homologies. ProcNatl. Acad. SciU. S. A. 54:491–498
    [Google Scholar]
  7. Krøll J. 1973; Rocket-line immunoelectrophoresis. 8387 Axelsen N. H., Krøll J., Weeke B. A manual of quantitative immunoelectrophoresis. Scand. J. Immunol. Suppl. 1 Universitetsforlaget; Oslo:
    [Google Scholar]
  8. Moore R. L., McCarthy B.J. 1967; Comparative study of ribosomal ribonucleic acid cistrons in enterobacteria and myxobacteria. J. Bacteriol 94:1066–1074
    [Google Scholar]
  9. Takahashi H., Saito H., Ikeda Y. 1967; Species specificity of the ribosomal RNA cistrons in bacteria. Biochim. Biophys. Acta 134:124–133
    [Google Scholar]
  10. Tchan Y. T., Wyszomirska-Dreher Z., Vincent J.M. 1980; Preliminary study of taxonomy of Azotobacter and Azomonas by using rocket line immunoelectrophoresis. Curr. Microbiol 4:265–270
    [Google Scholar]
  11. Thompson J. P., Skerman V.B.D. 1979; Azotobacteraceae: the taxonomy and ecology of the aerobic nitrogen-fixing bacteria. Academic Press, Inc.; London:
    [Google Scholar]
  12. Thompson J. P., Skerman V.B.D. 1981; Validation of the publication of new names and new combinations previously effectively published outside the USB. List no. 6. Int. J. Syst. Bacteriol 31:215–218
    [Google Scholar]
  13. Vincent J. M. 1974; Root-nodule symbioses with Rhizobium, p.. 265–341 Quispel A. The biology of nitrogen fixation North-Holland Publishing Co; Amsterdam:
    [Google Scholar]
  14. Vincent J. M. 1977; Rhizobium: general microbiology, p. 277–366 Hardy R. W. F., Silver W.S. A treatise on dinitrogen fixation, sect. III. Biology John Wiley & Sons, Inc.; New York:
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-33-2-147
Loading
/content/journal/ijsem/10.1099/00207713-33-2-147
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error