@article{mbs:/content/journal/ijsem/10.1099/00207713-33-2-147, author = "TCHAN, Y. T. and WYSZOMIRSKA-DREHER, Z. and NEW, P. B. and ZHOU, J.-C.", title = "Taxonomy of the Azotobacteraceae Determined by Using Immunoelectrophoresis", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "1983", volume = "33", number = "2", pages = "147-156", doi = "https://doi.org/10.1099/00207713-33-2-147", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-33-2-147", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "The similarities of various strains of Azotobacter spp. and Azomonas spp. to reference strains of Azotobacter paspali, Azotobacter vinelandii, Azotobacter chroococcum, Azomonas agilis, Azomonas insignis, and Azomonas monocytogenes were determined by rocket line immunoelectrophoresis. The strains of Azotobacter paspali and Azotobacter vinelandii used were immunologically more homogeneous than the strains of Azotobacter chroococcum studied, possibly due to the more diverse geographical origins of the Azotobacter chroococcum strains. Low values were obtained for the mean immunological distances (1 – proportion of immunoprecipitation bands shared between strains) between Azotobacter paspali and Azotobacter vinelandii strains, suggesting that these two species are immunologically closely related. Immunological distances from the Azotobacter chroococcum reference strain were similar for Azotobacter paspali and for other undisputed members of the genus Azotobacter, which makes it reasonable to retain Azotobacter paspali in this genus. When the three Azotobacter antisera were used, all Azotobacter species had mean immunological distances of less than 0.5, whereas the Azomonas species were immunologically more distant, showing that the six species of Azotobacter form an immunologically related group which is distinct from the Azomonas species. Our results with the three Azomonas antisera show that each species of Azomonas is immunologically distant from the other species, as well as from the Azotobacter species. We compare our immunoelectrophoretic results with the molecular biological results of De Smedt et al. (Int. J. Syst. Bacteriol. 30:106–122, 1980) and the numerical taxonomic analysis of Thompson and Skerman (Azotobacteraceae: the Taxonomy and Ecology of the Aerobic Nitrogen-Fixing Bacteria, Academic Press, Inc., London, 1979).", }