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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 8

sp. nov., a nitrate-reducing and thiosulphate-oxidizing bacterium, isolated from coastal sediment No Access

Abstract

A novel bacterial strain, N1Y112, was isolated from coastal sediment collected in Weihai, PR China. This Gram-stain-negative, facultatively anaerobic, motile rod-shaped bacterium exhibited the ability to oxidize thiosulphate to sulphate and reduce nitrate to ammonia through its Sox system and nitrate reduction pathway, respectively. The strain grew at 20–35 °C (optimum, 28 °C), pH 6.0–10.0 (optimum, pH 7.5) and in the presence of 1.0–5.0 % (w/v) NaCl (optimum, 3.0 %). Major fatty acids present in the strain included summed feature 8 (comprising C 7 and/or C 6), summed feature 3 (comprising C 7 and/or C 6) and C. Its polar lipid profile consisted of one phosphatidylethanolamine, two unknown aminolipids, one aminophosphoglycolipid, one diphosphatidylglycerol, one phosphatidylglycerol, two unknown phospholipids and two unknown lipids. Strain N1Y112 contained ubiquinone-7 and ubiquinone-8 as isoprenoid quinones, with a genomic G+C content of 50.6 mol%. Based on phylogenetic analysis, strain N1Y112 clustered with JCM 30316 being its closest relative at 97.1 % 16S rRNA gene sequence similarity. The average nucleotide identity and digital DNA–DNA hybridization values were 77.1 and 20.7 %, respectively, which suggest significant differences between genomes of N1Y112 and JCM 30316. Based on the findings from its phenotypic, genotypic and phylogenetic analyses, N1Y112 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is N1Y112 (=KCTC 72927=MCCC 1H00429).

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  • Published Online:
Keyword(s): draft genome sequencing , nitrate reduction , Pontibacterium and thiosulphate oxidization
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 32070002)
    • Principle Award Recipient: Zong-JunDu
  • National Science and Technology Fundamental Resources Investigation Program of China (Award 2019FY100700)
    • Principle Award Recipient: Zong-JunDu
© 2023 The Authors
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2023-08-23
2024-05-09
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Pontibacterium sinense sp. nov., a nitrate-reducing and thiosulphate-oxidizing bacterium, isolated from coastal sediment
Int J Syst Evol Microbiol 73, 006018 (2023); https://doi.org/10.1099/ijsem.0.006018
/content/journal/ijsem/10.1099/ijsem.0.006018
/content/journal/ijsem/10.1099/ijsem.0.006018
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