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Volume 73, Issue 8

gen. nov., sp. nov., an haloalkaliphilic actinobacterium isolated from seawater of the Mariana Trench No Access

Abstract

A Gram-stain-positive, strictly aerobic, rod-shaped actinobacterium, designated strain ZYF776, was isolated from seawater of the Mariana Trench collected at a depth of 4000 m. Results of 16S rRNA gene sequence analysis indicated that strain ZYF776 was a member of the class and closely related to DSM 45188 (member of the order , 94.94 % sequence similarity) and KCTC 33612 (member of the order , 94.46 %). Strain ZYF776 was catalase-positive and oxidase-negative. Growth occurred at 16–37 °C (optimum, 28 °C), in the presence of 0–13 % NaCl (w/v; optimum, 4 %) and at pH 7.0–10.0 (optimum, pH 8.0). Cell-wall hydrolysates of strain ZYF776 contained -diaminopimelic (peptidoglycan type A1γ), with ribose, rhamnose and a smaller amount of xylose as the cell-wall sugars. The major menaquinone was MK-10. The predominant fatty acids (>10 %) were C, C 8 and summed feature 3 (C 7 and/or C 6). The polar lipid profile mainly contained diphosphatidylglycerol, phosphatidylglycerol and phosphoglycolipid. The genomic DNA G+C content of strain ZYF776 was 68.7 mol%. The genome of strain ZYF776 was about 5.61 Mbp in size, which was larger than those of the reference strains DSM45188 (5.56 Mbp) and KCTC 33612 (3.98 Mbp). The average nucleotide identity and digital DNA–DNA hybridization values between ZYF776 and the related strains DSM 45188 and KCTC 33612 were 76.7 and 20.3 % and 75.8 and 20.0 %, respectively. Based on the polyphasic evidence, a novel genus and species with the name gen. nov., sp. nov. is proposed. The type strain is ZYF776 (=JCM 33008=MCCC 1K03555).

  • Received:
  • Accepted:
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Keyword(s): Mariana Trench , Profundirhabdus halotolerans and seawater
Funding
This study was supported by the:
  • Fundamental Research Funds for the Central Universities (Award 202172002)
    • Principle Award Recipient: Xiao-HuaZhang
  • National Key Research and Development Program of China (Award 2018YFE0124100)
    • Principle Award Recipient: Xiao-HuaZhang
  • National Natural Science Foundation of China (Award 41730530)
    • Principle Award Recipient: Xiao-HuaZhang
  • National Natural Science Foundation of China (Award 92251303)
    • Principle Award Recipient: Xiao-HuaZhang
© 2023 The Authors
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2023-08-23
2024-05-08
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Profundirhabdus halotolerans gen. nov., sp. nov., an haloalkaliphilic actinobacterium isolated from seawater of the Mariana Trench
Int J Syst Evol Microbiol 73, 006016 (2023); https://doi.org/10.1099/ijsem.0.006016
/content/journal/ijsem/10.1099/ijsem.0.006016
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